A technique that can be used to separate proteins based primarily on the presence of non-polar residues on their surface is called A) ion-exchange chromatography. B) gel filtration chromatography. C) affinity chromatography. D) gel electrophoresis. E) hydrophobic interaction chromatography.

Answers

Answer 1

Answer:

E) hydrophobic interaction chromatography.

Explanation:

Hydrophobic interaction chromatography (HIC) is a methodology used to separate protein molecules depending on their hydrophobicity. HIC enables the separation and purification of proteins by using a reversible interaction between the target protein and the hydrophobic ligand of a HIC resin such as, for example, methacrylate-based beads. This interaction is influenced by the running buffer (high salt concentrations favor hydrophobic interactions). HIC enables the separation of proteins under low denaturation conditions, thereby preserving their biological functions.


Related Questions

which of the following is NOT a type of volcano?

Answers

Answer:

Eliminate from the following

Explanation:There following are the only types of volcanos

Cinder cones,composite volcanoes,shield volcanoes and lava domes

Answer:

Explanation:
Pictures?

Help!!

How are all forms of asexual reproduction similar?
A. The offspring have identical DNA to the parent.
B. The offspring have different DNA than the parent.
C. They all require two parents to reproduce.
D. The offspring have diversified DNA in order to survive

Also explain why the answers are correct/incorrect.

Answers

Answer:

Asexual reproduction produces offspring that are genetically identical to the parent because the offspring are all clones of the original parent. This type of reproduction occurs in prokaryotic microorganisms (bacteria) and in some eukaryotic single-celled and multi-celled organisms

Hope this helped!!!!

Answer:

Asexual reproduction produces offspring that are genetically identical to the parent because the offspring are all clones of the original parent.

So A.

B. incorrect because they have the same DNA

C. incorrect because They do not need 2. it produces offspring that are genetically identical to the parent because the offspring are all clones of the original parent.

D. incorrect because They do not have diversified DNA

The polymerase chain reaction, or PCR, is a tool used frequently in biotechnology. Because PCR mimics the natural DNA replication that occurs in the cell, the two processes share many characteristics. Sort the descriptions of these processes by dragging them to the appropriate boxes. The descriptions may apply to natural replication only, to PCR only, or to both processes.

Answers

Answer:

PCR

Produces million of copies of  a segments of DNA and

Requires cycles of temperature.

PCR.

When large number of copies of a particular gene fragment is needed from smallest quantity of DNA molecules,the techniques of genetic engineering usually employed is called PCR. It involves denaturation of template DNA,the use of Primers(20 base pairs),the use of enzyme DNA polymerase for building of new strands of DNA.

Natural Replication.

produces a copy of the whole genome

It occurs before mitosis and meiosis

Begins at the origin of replication.

Both.

Requires primers

Requires DNA polymerase

Results in Semi conservative replication

Explanation:

When  rapid production of large copies of particular DNA fragments is needed from a small molecules of fragment of the original DNA fragments is needed the tool of genetic engineering used is called  PCR.

It involves denaturation of the template DNA at 95 degrees for separation to single strands,

the attachments of primers(20 base pairs of complementary bases to the template DNA) at 65 degree a process called annealing and the use of polymerase enzyme for building on chains of DNA , during elongation.

The enzyme are extracted from thermophilic organisms,therefore could withstand the high temperature.The polymerization can proceed for a continuous cycle with million of new DNA chains produced by the polymerization Chain  machine

What concept from microbiology is the most challenging and why? What can you do to learn more about this concept as you progress through nursing program and career?

Answers

Answer:

The overview of the particular topic is discussed in the section through the below clarification.

Explanation:

Microbiology seems to be the branch of philosophy that deals with some really small sentient creatures like bacteria as well as about there impact on human health. The profession deals with the analysis, component, as well as classification of such species, including to exploit as well as regulate their transactions.Nevertheless, their using it as manufacturing applications is usually a complex idea of microbiology. To understand everything concerning this idea, you will concentrate on several aspects of biotechnology to properly appreciate how would control micro-organisms to generate valuable material.

Complete the following.
1. Waste containing dangerous chemicals is ...... waste.
2. Vegetable and fruit peels and food left over comprise ... waste.
3. Hospital waste is highly ......
4. Plastic is nonbiodegradable, as it does not
5. The method of adding earthworms to compost pits is called ...​

Answers

1) Waste that contains both hazardous chemicals and radioactive wastes (generally those in the LLW category) is termed 'mixed waste

2) Vegetable and fruit peels and food left over comprise biodegradable waste.Plastic is non-biodegradable, as it does not decompose

3) Hospital waste is the second most hazardous waste after radioactive waste. The improper management of hospital wastes causes serious environmental problems in terms of air, water and land pollution

4) most plastics are non-biodegradable mainly because plastic is widely used because of its low cost, versatility and durability

5) Vermicompost (vermi-compost) is the product of the decomposition process using various species of worms, usually red wigglers, white worms, and other earthworms, to create a mixture of decomposing vegetable or food waste, bedding materials, and vermicast

hope it's helpful to you

Metallic bonds are responsible for many properties of metals, such as conductivity. Why is this possible? (1 point)
O Metals have low electronegativity, so they are conductive because they pass charges easily.
OTwo metals bonded together are going to be more conductive than a metal bonded with a nonmetal.
O The bonds can shift because valence electrons are held loosely and move freely.
O An attraction between a positive charge and a negative charge is created.

Answers

The correct answer is;  Metals have low electronegativity, so they are conductive because they pass charges easily.

Metals are found towards the left hand side in the periodic table. They are known for their low electronegativity. Metals are electropositive. This means that they loose charges(electrons) easily.

A metallic bond is comprised of positively charged metallic ions and a sea of electrons.These electrons can move freely.

This sea of electrons are the charges that are responsible for the properties of metals such as high conductivity.

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If a scientific journal article is difficult to understand in its entirety, what is the best resource for comprehending the overall study
and results?

Answers

Answer:B an abstract of the study

Explanation:

From Scenario t What is the Dependent Variable? *
the flower
the flower color
O the flower with no fertilizer
when the flower blooms

Answers

Answer:

The flower color

Explanation:

Answer:

The flower color

Explanation:

A retired contract administrator who enjoyed gardening sought medical attention for what appeared to be a sinus infection. He received antimicrobials but the conditioned worsened and he was experiencing severe painful spasms in his jaw. He admitted to injuring himself with a gardening tool while wearing sandals in the yard but did not seek medical attention for the wound. The man is likely experiencing.
A. allergic rhinitis.
B. meningitis.
C. septic shock.
D. LPS-mediated inflammatory response.
E. intoxication caused by a focal C. tetani infection.

Answers

Answer:

The correct answer is E. intoxication caused by a focal C. tetani infection.

Explanation:

Clostridium tetani bacteria are found in the soil, in the feces and in the mouths of animals. The disease is acquired when wounds become infected by the bacteria. The spores become active and spread throughout the body producing a poison called tetanus toxin. This toxin blocks the nerve signals from the spinal cord to the muscles and can cause generalized severe muscle spasms (lockjaw) exacerbated by external stimuli or muscle spasms in areas adjacent to the wound.

Individual "A" produces 10 offspring, 8 of whom survive to adulthood. Individual "B" (a member of the same population) produces 15 offspring, 7 which survive to adulthood. Based on the concept of natural selection, individual "A" has the higher fitness.a) trueb) false

Answers

Individual a has the higher fitness because more of its offspring survived compared to the original amount of offspring

In natural selection when more offspring is survived is a sign of higher fitness of the particular population, hence given statement is true.

What is natural selection?

Most qualities are inherited, there are more children generated than can survive, and offspring with more advantageous features will survive and have more offspring than those with less advantageous traits leading to the inevitable process of natural selection.

Since there are only so many resources available, more creatures are born than can live those who are better at obtaining food.

Mating or evading predators will have a higher chance of thriving, procreating, and passing on their DNA.

Therefore an individual in the population has offspring to survive more in number.

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what are quantities? Give any two example.​

Answers

Answer:

Physical quantities that are derived from one or more fundamental physical quantities are called derived physical quantities. Examples: area, volume, speed, density , etc.

Explanation:

Hope this helps. Have a nice day^_^

How does coronavirus causes severe pneumonia?

Answers

Answer:

please mark me brainliest

Explanation:

The new coronavirus causes severe inflammation in your lungs. It damages the cells and tissue that line the air sacs in your lungs. These sacs are where the oxygen you breathe is processed and delivered to your blood.

Answer:

The new coronavirus causes severe inflammation in your lungs. It damages the cells and tissue that line the air sacs in your lungs. These sacs are where the oxygen you breathe is processed and delivered to your blood. The damage causes tissue to break off and clog your lungs

Explanation:

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the liquid produced by the skin that helps to cool down the body​

Answers

Answer:

The sweat leaves your skin through tiny holes called pores. When the sweat hits the air, the air makes it evaporate (this means it turns from a liquid to a vapor). As the sweat evaporates off your skin, you cool down.

Please help me...
Blonde hair (represented as b) is a recessive trait. If Julie has blonde hair, which of the following could have been her parents' genotypes?
A. Bb, bb
B. BB, bb
C. BB,BB
D. Bb,BB

Answers

I think its Bb, bb because if julie has blonde hair then it's most likely to be Bb, bb

If Julie has blonde hair, the genotypes of their parents could have been found to be Bb and bb. Thus, the correct option for this question is A.

What do you mean by Genotypes?

Genotypes may be defined as the ultimate combination of alleles of those genes which are selected for specific studies in order to express some genetic characteristics.

According to the context of this question, if the trait of Blonde hair is recessive in nature. This means that both the alleles of this gene are recessive, i.e. bb.

Now, if Julie has blonde hair, which means both alleles are recessive. So, the genotypes of parents of Julie are either homozygous recessive or heterozygous in nature. This characteristic is well determined by option 1.

Therefore, if Julie has blonde hair, the genotypes of their parents could have been found to be Bb and bb. Thus, the correct option for this question is A.

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Which type of graph would have a line of best fit?
O A. Scatter plot
OB. All of these
OC. Bar graph
D. Variable plot

Answers

Answer:

Scatter Plot

A line of best fit is a straight line that best represents the data on a scatter plot.

This line may pass through some of the points, none of the points, or all of the points.

Answer:

Option B

Explanation:

Scatter plots and variable plots would have lines of best fits to approximate the data shown on the graph accurately.

A line of best fit on a bar graph is called a trendline. A trendline is a line or curve that shows the overall pattern for data on a bar graph.

Option B should be the best answer.

Hope this helps.

The poles experience 6 months of daylight and 6 month of darnos . The sun is never higher than 23.5 degrees above the horizon . Use one or both of these ideas as evidence to explain why the season are most extreme at the poles

Answers

Answer:

North and South pole are cold as they do not receive direct sunlight. South pole is much colder than North pole. The Sun is always low on the horizon, even during summer and winters.

The Sun only comes up for a few months, that is why both nights and days are dark and cold.

The average mean temperature in summers at North pole is 32 degrees Fahrenheit and in winters its minus 40 degrees. While at south pole it is minus 18 degrees Fahrenheit and in winters it is minus 76 degrees Fahrenheit.

the water is..........​

Answers

Answer:

The water is chemical compound.

Do you agree or disagree, why or why not, give examples. The knowledge produced by science builds on old ideas and is constantly changing.

Answers

Answer:

agree

Explanation:

people are never going to stop learning

things will evolve

what has been discovered in the past is still relevant

The knowledge produced by science builds on old ideas and is constantly changing agree.

What is Science?

Science is a methodical process that creates and arranges knowledge in the form of universe-related explanations and predictions that can be put to the test.

Some of the earliest archeological evidence for scientific reasoning is tens of thousands of years old, and science may be as old as the human species.

In the period between 3000 and 1200 BCE, Mesopotamia and Ancient Egypt produced the oldest written documents in the history of science. Greek natural philosophy of classical antiquity, where formal attempts.

Therefore,  The knowledge produced by science builds on old ideas and is constantly changing agree.

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Which type of chromosomal disorders seems to have the greatest affect on a person’s health—disorders involving autosomes or sex chromosomes? Why do you think this might be the case? thanks in advance

Answers

Answer:

Disorders involving sex chromosomes.

we can take Turner's syndrome n klinefelter's syndrome as examples.

100 points! will mark BRAINLIEST!!!!!!! Cellular respiration transforms glucose and oxygen into carbon dioxide, water, and energy.



C6H12O6 + 6O2 → 6CO2 + ?H2O + Energy



Based on the law of conservation of matter, what is the missing coefficient for water?





6


2


8

4

Answers

Answer:

your answer is 6

Explanation:

C6H12O6 + 6O2 → 6CO2 + ?H2O + Energy

H12O6---> 2 hydrogen and 1 oxygen for very water molecule. 12 ÷ 2= 6 which means 6H2O

CH2OH О, a CH3 H OH OH Н он Identify the macromolecule pictured above. O a Ob Protein Carbohydrate Lipid , ос Od Nucleic Acid​

Answers

Answer:

Although the pictorial representation is not clear but the question can be answered based on the known structure of each biomolecule. The answer is:

Carbohydrate

Explanation:

Carbohydrate is one of the four biological molecules in living systems. Carbohydrates are composed of long chains of carbon, hydrogen and oxygen in a 1:2:1 ratio i.e (CH2O)n empirical formula.

The structure of carbohydrate is composed of a ketone (C=O) or aldehyde (CHO) functional group and hydroxyll groups (OH). The simplest unit of any carbohydrate is called a monosaccharide. Hence, from the above structure in the question, which consists of long chain of carbon, hydrogen and oxygen atoms, it can be identified as a CARBOHYDRATE.

what factors do you think determine the age of the universe

Answers

The trees being so tall, eruption on rocks shows that the earth has aged, and weathering. Because scientists can track down years from these things listed

Which of the following is an example of population growth? Group of answer choices the average weight of Americans has increased substantially in the past decade. tropical fish have been found in more northern waters than their usual habitat. the number of people in a town has increased by 25% in the past 5 years. the number of butterflies in a region have stayed the same from 1950-2010.

Answers

Answer:

the number of people in a town has increased by 25% in the past 5 years.

Explanation:

Hope it helps.

Which structure is located between the trachea and a bronchiole? A. epiglottis B. pharynx C. alveolus D. bronchus

Answers

the correct answer is the bronchus

How the population environment and health are related with different aspects? Elaborate

Answers

Answer:

The aspects of that natural resources like air, water, soil etc aspects include the existence,these aspects of population health and environment.

Explanation:

Population environment and the health is to the approach human development to conservation, health, population is the deep relationship that not only related to each other.

population environment or health is the process can perform effect of population growth in health and environment aspects, other hand population controlled balanced a environment, produce healthy people.

Biological aspect :- health and population are related with the biological aspects of that environment, health, and population aspects of environment related to each other.

Social aspects:- social and cultural aspects like to the festivals, tradition living standards  aspects environment, population and health are related each other.

Physical aspect:- the development construction bridge,canals, industries that are physical environment, these aspect interrelated health and population.

Economic aspect :- economic aspect is reflected by the health of the people,resources like water, plants, land.  

If the percent concentration is greater in the fluid outside a cell than inside a cell, how will the water
flow?
This means the solution would be___________
Which tube(s) did this happen to?

Answers

Answer:

The water will flow from the inside of the cell to the fluid outside the cell. This means that the solution would be hypertonic.

It will happen to the tubes that have a higher concentration of solutes in the fluid outside cells than inside the cells.      

Explanation:

When a cell is in a hypotonic solution, it means that the concentration of solute in the solution is higher than the one in the cell. As a natural reaction, the cell tries to balance this difference. To do this, the water that is inside the cell flows to the outside. As a result, the concentration between the inside and the outside of the cell becomes equal or more equitable. Besides, the cell shrinks due to the loss of water.

Which of the following statements are true about antibodies?

A. Both Polyclonal and Monoclonal antibodies are naturally produced in immune
responses.
B. Polyclonal antibodies are part of the natural immune response; monoclonal antibodies
are clones of plasma cells used for medical research.

C. Polyclonal antibodies are used for the treatment of cancer, monoclonal antibodies are
used for medical research.
D. Monoclonal antibodies work against many types of antigens; polyclonal antibodies
work against a specific antigen.

Answers

Answer:

both a and b are the correct answers

How you complete this

Answers

Answer:

Hi. Can you type the question, it's a bit confused reading it like this thank you!.

an example of biogeochemical cycle would be the A. carbon cycle B. fire cycle C. trophic cycle

Answers

Answer:

Carbon Cycle

Explanation:

what are the structural adaptation of whales and dolphins (compare them please)

Answers

Answer:

Whales and dolphins are mammals and have lungs instead of gills for breathing. They have blowholes at the tops of their heads that function like nostrils; they need to put their heads above water every so often to breathe. Both dolphins and whales have hair instead of scales, another quality that is specific to mammals. The animals have live births instead of laying eggs, and baby dolphins and whales nurse from their mothers. Whale and dolphin births take place near the surface of the water, so the young animals can easily take their first breath.

Both whales and dolphins rely on the sounds they make for survival, even though the sounds are utilized differently by each animal. For instance, whales make high-pitched groans and whistles to communicate with one another to warn against danger or navigate during migration

Answer:

COMPARISON AND CONTRAST OF WHALES AND DOLPHINS

Both dolphins and whales belong to the cetacean family and are marine animals that reside in the ocean. Some scientists even assert that dolphins are a type of whale, as the two animals have a number of shared characteristics. However, there are distinct differences between whales and dolphins as well that will teach you more about the physical appearance of these marine creatures and how they survive in their environment.

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