explain the differences between gel electrophoresis and column chromatography. address the principles behind each separation. why do large molecules migrate more easily in one method and with more difficulty in the other? which method generated the most precise results?

Answers

Answer 1

In the process of chromatography, the components of the sample are divided according to how evenly they disperse between a stationary phase and a mobile phase. Using using a variable rate of migration in an electric field, sample components are separated by electrophoresis.

The foundation of chromatography is the idea that mixtures of molecules applied to surfaces or solids, and fluid stationary phases (stable phases), separate from one another while moving with the help of a mobile phase.

The fundamental idea behind column chromatography is to use a stationary phase to help adsorb the solution's solutes before further separating the mixture into distinct components.

In gel electrophoresis the separation is by charge is to mass ratio and hence smaller molecules moves faster then larger molecules.

Chromatography generated the most precise results.

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Related Questions

hydrogen gas and nitrogen gas react to form ammonia gas. what volume of ammonia would be produced by this reaction if 2.9 ml of hydrogen were consumed?

Answers

On balancing the reaction of formation of ammonia we know that for every one mole of Nitrogen gas, we need three moles of Hydrogen gas to produce, two mole of the ammonia.

We are given with volume of Hydrogen gas i.e. 2.9 ml and are asked to determine the volume of ammonia

Since the volume of hydrogen is given as 2.9 ml = 2.9/1000 l

Mole of hydrogen =2.9/(22.4)(1000)

Mole of hydrogen = 0.129/1000 moles of H₂

Get the number of moles of NH₃

Number of moles of  NH₃ = 2/3 of moles of Hydrogen

Number of moles of  NH₃ = 2/3 * 0.129/1000 moles of H₂

Number of moles of  NH₃ =0.0863/1000 moles of  NH₃

Get the volume of ammonia  NH₃

Volume of ammonia produced = 0.0863 * 22.4

Volume of ammonia produced = 1.933 ml

Hence the volume of ammonia that would be produced by this reaction if 2.9 ml of hydrogen were consumed is 1.933 ml

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where was oxygen discovered?

Answers

Oxygen was discovered in Poland

Answer: nowhere from trees

Explanation:

an experiment called for l of sulfur dioxide, , at and atm. what would be the volume of this gas at and atm?

Answers

since you  have  Sulphur dioxide in the experiment    . Use Charles' Law,  to know the volume [tex]V 1 /T 1 = V 2/ T 2[/tex]

what is the volume of the gas ?

The volume of gas is defined as the space occupied by the gaseous particles at standard temperature and pressure conditions. It is denoted as 'V'. The SI unit of volume is 'Litres' denoted as 'L'. A mole of gas has a volume  at room temperature.Boyle's Law - states that the volume of a given amount of gas held at constant temperature varies inversely with the applied pressure when the temperature and mass are constant.Volume = length x width x height.You only need to know one side to figure out the volume of a cube.The units of measure for volume are cubic units.Volume is in three-dimensions.You can multiply the sides in any order.Which side you call length, width, or height doesn't matter.The volume of a gas is directly proportional to its temperature when pressure is constant. The ratio of volume to temperature is constant when pressure is constant. This relationship is known as Charles' law or Gay-Lussac's law .

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which of the following changes occur when an acid solution of unknown concentration is titrated with a standardized solution of naoh? select all that apply. multiple select question. the color of the indicator slowly changes over the course of the titration. the ph of the acid solution decreases. the concentration of h ions in the acid solution decreases. the ph of the acid solution increases.

Answers

The colors of the starting solutions differ in case of high and low concentrations, because the titrations start at different pH values.

The colors of the starting solutions vary in case of high and coffee concentrations, due to the fact the titrations start at distinctive pH values. In case of excessive concentrations, the starting color is of brilliant pink red, converting to purple orange all through innovative titration.

The signs have a chemical structure which can soak up mild beneath positive pH conditions and both change shade or grow to be colorless below other pH situations. This normally happens because of a exchange in protonation of the purposeful group of the molecule.

Whether an answer is acidic or simple can be measured at the pH scale. When everyday indicator is brought to a solution, the color change can indicate the approximate pH of the solution. Acids purpose universal indicator option to trade from green in the direction of crimson.

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2. after work-up, the product of the wittig reaction was recrystallized from hot isopropanol. you were told to do a gravity filtration using a heated stemless funnel if the solution appeared cloudy. what was the likely identity of compound that caused the cloudiness? it was also indicated that the filter should be washed with hot isopropanol if crystals started to form on the stemless funnel. why would hot isopropanol be used instead of cold isopropanol

Answers

The molecules of isopropyl alcohol do not stick collectively as strongly at room temperature as water molecules do, because of this the alcohol evaporates more quickly than water does.

DNA isn't soluble in ethanol, consequently, it will clump collectively and come to be visible. The colder the ethanol, the less soluble the DNA can be in it, which is why it's far critical for the ethanol to be kept bloodless.

Isopropyl Alcohol may also shape an ignitable vapor/air combination in closed tanks or boxes. Isopropyl Alcohol can react with AIR and OXYGEN over the years to shape unstable peroxides which can explode. Isopropyl Alcohol forms explosive combos, whilst heated, with ALUMINUM.

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what three flaws of the traditional periodic table does jeff moran feel he has addressed with his periodic spiral

Answers

The three major flaws addressed by Moran's periodic spiral are:

1) Since hydrogen possesses distinct attributes that set it apart from every other element in the table, it effectively does not belong to any of the table's 18 columns.

2) The lanthanides and actinides, which are the elements from each period of the f block, have comparable properties but are not shown in columns in the table.

3) The column-and-row layout of the  periodic table hides the continuity between the pieces, which is better represented by an a spiral.

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Is a process that is internally reversible and adiabatic necessarily isentropic?.

Answers

No, a process that is internally reversible and adiabatic is not necessarily isentropic. A process is isentropic if there is no change in entropy during the process. However, a process can be internally reversible and adiabatic and still have a change in entropy.

Entropy is a measure of the disorder of a system, and a reversible process can still have a change in entropy if the system is not in equilibrium.A reversible process is one that can be reversed without any change in entropy. However, if the system is not in equilibrium, the process can still have a change in entropy. This is because the entropy of a system is a measure of the disorder of the system, and a system that is not in equilibrium is not in a state of maximum disorder.

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For a certain farming community in California, a water shortage is predicted if the average global temperature rises by 1 degree Celsius. The residents of this community rely on private vehicles powered by fuels for transportation because there is no public transportation system. Farm vehicles powered by diesel fuel are used to harvest crops and get them to market. Residents of the farming community want to reduce their impact on global temperature increases by reducing their carbon dioxide emissions. Residents of the farming community want to reduce their impact on global temperature increases by reducing their carbon dioxide emissions.

Choose the answers below that are true in regards to this scenario.

Question 2 options:

Residents of this community drive personal fuel-powered vehicles.


Farmers in this community use diesel-powered farm vehicles.


Residents in this community are NOT concerned about reducing their CO2 emissions.

Answers

The answers that are true in regards to the scenario above is:

Residents of this community drive personal fuel-powered vehicles.Farmers in this community use diesel-powered farm vehicles.

So, the first and second options are correct. The third and last option is incorrect.

What is a community?

A community is described as a social unit with commonality such as place, norms, religion, values, customs, or identity.

From the scenario above, we can conclude the following:

residents of the farming community want to reduce their impact on global temperature increases by reducing their carbon dioxide emissions. Residents of this community drive personal fuel-powered vehicles.Farmers in this community use diesel-powered farm vehicles.

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the separator breaks down making the process isobaric. will the benzene composition of the liquid leaving the separtor increase, decrease, or stay the same?

Answers

The process is isobaric as a result of the separator breaking down. The amount of benzene in the liquid leaving the separator increases, This is also known as isobaric process.

A thermodynamic process known as an isobaric process occurs at a fixed pressure. Greek words "iso" and "baros," which both signify equal pressure, were used to create the phrase "isobaric." Therefore, when the volume is increased or decreased, the constant pressure is obtained.

With the molecular formula C6H6, benzoene is an organic chemical substance. Six carbon atoms linked together in a planar ring, with one hydrogen atom connected to each, make up the benzene molecule. Benzene is a hydrocarbon since it is made up only of carbon and hydrogen atoms.

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the reaction between copper and silver nitrate produces beautiful silver flakes and a bright blue copper (ii) nitrate solution according to the following reaction: cu 2agno3 2ag cu(no3)2 how many moles of agno3 will be needed to completely react with 10.0 g of cu?

Answers

0.3147128245 moles of AgNO₃ will be needed to completely react with 10.0 g of Cu.

Determine the number of moles of copper by dividing its mass by its molar mass.

moles Cu = mass Cu / MM Cu

moles Cu = 10.0 g/ 63.55 g/mol

moles Cu = 0.1573564123

Based on the balanced chemical reaction between copper and silver nitrate, 1 mole Cu needs 2 moles AgNO₃ to proceed with the reaction completely.

Cu + 2AgNO₃ ⇒ 2Ag + Cu(NO₃)₂

If there are 0.1573564123 moles Cu, then 0.3147128245 moles AgNO₃ is needed.

moles AgNO₃ = 0.1573564123 moles Cu(2 moles AgNO₃ / 1 mole Cu)

moles AgNO₃ = 0.3147128245

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why is the extrapolation method for determining the titration end point is more accurate than finding the minimum conductivity

Answers

Extrapolation method for determining the titration end point is more accurate than finding the minimum conductivity because there is no human error when calculating as there could be with doing the actual experiment

Extrapolation is the estimating an unknown value based on extending a known sequence of values and to extrapolate is the infer something not explicitly stated from existing information and the point at which the indicator changes color is called as endpoint and so the addition of an indicator to the analyte solution help us to visually spot the equivalence point in an acid base titration and the endpoint refer to the point at which the indicator changes color in and acid base titration

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ammonia (nh3) chemically reacts with oxygen gas to produce nitric oxide and water. what mass of nitric oxide is produced by the reaction of 2.81 g of ammonia?

Answers

The mass of nitric oxide produced by the reaction of 2.81 grams of ammonia is 1.59 grams.

The equation for the reaction of ammonia and oxygen to produce Nitric oxide and water is,

4NH₃ + 5O₂ → 4NO + 6H₂O

From the reaction we can see,

4 moles of ammonia = 4 moles of nitrix oxide.

Mole of ammonia = moles of nitric oxide.

Moles = reacted/formed mass/ molar mass

Molar mass of ammonia = 17 g/mol.

Molar mass of nitric oxide = 30 g/mol.

Mass of ammonia reacted = 2.81 grams.

Putting values,

2.81 x 17 = Formed mass of nitric oxide x 30

Formed mass of nitric oxide  = 1.59grams.

The mass of nitric oxide formed is 1.59 grams.

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Identify the following as metals, nonmetals or metalloids using the periodic table.
a. Silicon
b. fluorine
c. uranium
d. mercury
e. arsenic
f. iridium

Answers

Correct Answer:
a) metalloid
b) non-metal (halogen)
c) metal (actinides)
d) metal (transition metal)
e) metalloid
f) métal (transition metal)

I would appreciate it if you mark my answer as brainliest.

An alloy of bronze was made by combining 88 % copper and the rest is composed of tin. Determine the density of the bronze sample using a weighted average.” 8.76g/cm

Answers

Based on the densities and percent mass of copper and tin, the density of Bronze is 8.76 g/cm³.

What is density?

Density is the ratio of the mass and volume of a substance.

Density = mass/volume

For a mixture, the density of the mixture is the weighted average of the densities of the substance in the mixture.

The density of the bronze is calculated as follows;

The density of bronze = percent mass of copper * density of copper + mass of tin * density of tin

The density of copper = 8.96 g/cm³

Percent mass of copper = 88%

The density of tin = 7.3 g/cm³

Percent mass of tin = 12%

The density of Bronze = 88% * 8.96 + 12% * 7.3

The density of Bronze = 8.76 g/cm³

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24 points
Which two particles have opposite charges?
O A. lons and neutrons
B. Electrons and protons
OC. Neutrons and protons
OD. Electrons and neutrons

Answers

Answer- B
Took the test

Answer:B

Explanation: according  to the law of electrostatics it states that  "unlike charge attract each other and like charges repel each other ".

when energy-depleted elements associated with a proton are accepted by an organic molecule, the process is called

Answers

When energy depleted elements associated with a proton are accepted by an organic molecule the process is called Fermentation.

Fermentation is a metabolic process that causes a chemical change inside body by the actions of enzymes.

The main purpose of fermentation is to generate NAD+ and 32 ATP in the presence of oxygen.

Fermentation allows cells to survive without using ATP.

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4.why benzoic acid showed a smaller rf value than 9-fluorenone in the tlc result? explain thoroughly for full credit.

Answers

Benzoic acid showed a smaller rf value than 9-fluorenone in the TLC result because of its different attraction phase, stationary phase, and mobile phase.

The Rf esteem depends on the stationary stage and portable stage of the TLC plate. The Rf esteem of a compound changes based on how pulled it is to the stationary and versatile stages.

As a rule, the stationary stage is silica gel, which is very polar, so the versatile stage is moderately non-polar.  

Benzoic corrosive is moderately polar since it incorporates a carboxylic corrosive bunch. So, it would have a moo Rf value on a TLC plate with silica gel as the stationary stage and hexane as the versatile stage.

The Rf esteem on a TLC plate with silica gel as the stationary stage and 60:40 hexane: ethyl acetic acid derivation as the versatile stage.

The benzoic corrosive would be more pulled into the generally polar 60:40 hexane: ethyl acetic acid derivation than the non-polar hexane portable stage.

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Physical change involves a change in composition.

True
False

Answers

A physical change does indeed involve a change in composition. Hence,t the given statement is true.

A physical change is one in which the form of the matter is altered but this does not mean it transforms into another. By composition, we refer to the size and the shape of the matter which is altered after a physical change occurs.

A physical change is reversible. This means that even though the shape and size of the matter change completely, it can always be reverted back to how it was by applying all the conditions that were present there before.

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what kind of compound would 5A and 7A make

Answers

In periodic table group 5A consist of nitrogen, phosphorus, arsenic and the group 7A consist of all the halogens.

What is periodic table ?

The periodic table is a table form of chemical elements arranged according to atomic number, starting with hydrogen and ends to oganesson, which has the highest atomic number.

Because they are arranged in table form  of the elements, it is known as the periodic table. They're present in rows and columns. Periods and Groups are the names given to the horizontal rows that comes from left to right and the vertical columns that comes from top to bottom.

Thus, Group 5A contain nitrogen, phosphorus , arsenic compounds while in group 7A contain all the halogens.

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What is the reduced element of H2O2 + PbS = PbSO4 + H2O

Answers

Answer:

4H₂O₂ + PbS = PbSO₄ + 4H₂O

the standard gibbs free energy of formation of is zero. (a) h2o(l) (b) k(s) (c) cl2(g) the standard gibbs free energy of formation of is zero. (a) (b) (c) (a) only (b) only (c) only (b) and (c) (a), (b), and (c)

Answers

The standard Gibbs free energy of formation of is zero are option (b) K(s) and (c) Cl₂(g).

Gibbs free energy may be more than zero, much less than zero, and identical to zero. If Gibbs free energy is more than zero the response is a nonspontaneous reaction. If Gibbs free energy is less than zero the reaction is a spontaneous reaction.

The usual Gibbs free energy of formation of a compound is the exchange of Gibbs unfastened energy that accompanies the formation of 1 mole of that substance from its issue elements, of their well known states.

Gibbs free energy, G is a price that defines how spontaneous a response is, with a poor fee which means the reaction is spontaneous, and a advantageous cost that means the reaction is nonspontaneous. This fee is the combination of enthalpy and entropy.

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(c) Lithium oxide is an ionic compound.
Draw a dot and cross diagram to show how lithium and
oxygen combine to form lithium oxide.
Only show the electrons in the outer shell of each
atom.
Give the charges on the ions formed.

Answers

Answer:

Let us know formula and denotion for Lithium oxide is Li2O.

Explanation:

While on the basis of Electronic configuration:

Lewis structure(for Lithium atom)-Li•

For Oxygen atom- it is 6 dots in the Word O(for Oxygen) but is needed to paired in a pair(of two dots if possible) by the use of either all sides where two dots are presented two sides While one dot is represented on other two sides.

When we come across we might have a simple question how and why the Lithium oxide is Li2O

So,as we know Li(have one electron in its outermost shell),written as Li^+/+1 While Oxygen requires 2 more electrons to stabilize its valency,so it written as O^-2/2-

On combining,Li^+ & O^-2

Exchange and crossing up of valency is done forming Li2O.

Do check the image uploaded for better clarity and understanding.

How does the atomic radius change as you move left to right in Period 3 from aluminum through chlorine? Refer to the periodic table.

A. it generally increases.

B. it does not change.

C. it generally decreases.

D. it varies unpredictably.

Answers

Answer:

C it generally decrease

Explanation:

Atomic radius of an element decrease from left to right of the periodic table

14.what volume, in milliliters, of 6.0 m naoh is needed to prepare 175 ml of 0.20 m naoh by dilution?

Answers

5.83ml of NaOH is needed to prepare 175 ml of 0.20 m by dilution.

Dilution is the process of “lowering the concentration of a solute in a solution by simply adding more solvent to the solution, such as water.” Diluting a solution enables adding more solvent without adding more solute.

into formula to find out the volume is ;

[tex]V_{1}[/tex][tex]N_{1}[/tex] = [tex]V_{2}N_{2}[/tex]

where [tex]V_{1}[/tex]= ?

[tex]V_{2}[/tex] = 175ml

[tex]N_{1}[/tex] = 6

[tex]N_{2}[/tex] = 0.2

So, ⇒ [tex]V_{1}[/tex]×6 = 175×0.2

[tex]V_{1}[/tex] = 5.83 ml

So, 5.83ml of NaOH is needed to prepare 175 ml of 0.20 m by dilution.

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2
A student observed a sperm cell using a microscope at a
magnification of x400.
The sperm cell image measured 22 mm long.
Use the equation to calculate the real size of the cell.
Give your answer in micrometres (μm).
size of image
size of real object
219) 3120 20 2019) ST220M
magnification =

Answers

A student observed a sperm cell using a microscope at magnification of x400 and sperm cell image measured 22 mm long then the actual size is = 55 μm.

What is microscope and magnification?

A simple microscope produces an image of the object upon which the microscope is focused whereas magnification is the ability to make small objects seem larger such as making a microscopic organism also visible. Ability to distinguish two objects from each other is called as resolution

As we know that, 1 mm = 1000 μm.

So, 22mm = 22000 μm

Magnification of x400 given

We can calculate, Actual size = image size / magnification.

 = 22000/400

Actual size = 55 μm

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what volume in \pu{ml}ml of 6.00 \pu{m}m acetic acid is needed to make 200 \pu{ml}ml of 0.05 \pu{m}m acetic acid?

Answers

1.6 ml volume of 6.00 m acetic acid is needed to 200 ml of 0.05 m acetic acid

WE know that

V1 * M1 = V2 * M2

V2 = 200 ml = 0.2 l

M1 = 6.00 m

M2 = 0.05 m

V1 * 6m = 0.2 l * 0.05 m

V1 = (0.2 * 0.05) / 6

V1 = 0.0016 L

V1 = 1.6 ml

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how many outer atoms and lone pairs are present in a molecule with a square planar shape? outer atoms: lone pair(s):

Answers

The total outer atoms present in a molecule with planar shape are four and total lone pairs are 2.

Square planar shape is formed when there are four bonds and two lone pairs on the central atom.

The four atoms that are attached to the central atom are known as the four outer atoms. These atoms are arranged in such a way that it makes the square shape. The outer atoms are not always fours. They can be 2,3 or four.

The lone pairs are present on the central atom.

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2. A 12.2g sample of a hydrate was heated to a
constant mass of 10.2 grams. What is the
percent by mass of the water in the hydrate?

Answers

The percent by mass of the water in the hydrate is 83.6 percent.

Calculate the percent by the mass of each detail by using dividing the mass of that element by 1 mole of the compound by using the molar mass of the compound and multiplying by using one hundred%. consider your end result. the chances upload as much as a hundred%.

This system allows for displaying the bottom complete number of moles and the relative range of atoms of every detail in a compound. With the help of the empirical formulation, chemists can also calculate the actual molecular system. This component suggests the exact range of atoms within the compound.

Calculation:-

mass of hydrated sample = 12.2 gram

actual mass = 10.2

percentage mass = 10.2/12.2 × 100

                             =  83.6 percent.

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Sodium can combine with element "X" so that
the chemical formula is Na₂X. What element
could element "X" be?

A)Phosphorus
B)Helium
C)Magnesium
D)Sulfur

Answers

The element could be X is phosporus .

What is element?

An element is the fundamental item that can't be easily broken onto smaller of pieces. In chemistry and physics, an elementary is a substance that can't be broken down by non-nuclear reactions. In computing and the  mathematics, an element is the  distinct piece of a larger system or set.

Sol-An ionic compound is formed by the ions, which their charges replaced, so the compound Na₂X is formed by the ions Na⁺ and X⁻². Thus, X must gain two electrons to be stable at the octet, so it has 6 valence electrons. Because it can accommodate up to 12 electrons, the elememts expands the octet, do it must be in the 3rd period or higher.

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Calculate the time it will take to deposit 1 mole of silver if a current of 6A is passed through a solution of silver trioxonitrate (v) (F=96500)

Help!!!

Answers

The time taken to deposits one mole of silver if a current of 6 ampere is passed through the silver trioxonitrate solution is 16083 seconds or 283 minutes.

What is electrolysis?

Electrolysis is the process of converting electrical energy into chemical energy.  If current is passed between two electrodes which are immerse in an electrolyte solution, the metal which is reduced will deposited at the cathode.

The weight of the deposited metal w related with its equivalent weight z, current I and time t is :

w = zIt /96500.

One mole of silver is 108 g. Its equivalent weight also be 108 g Thus the time t is calculated as follows:

108 g = 108 × 6 × t /96500

      t = 96500/6

        = 160835 seconds or 268 minutes.

Hence, the time taken to deposits one mole of silver in this electrolysis is 268 minutes.

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