Genetic engineering involves various stages.

1 human DNA is inserted into bacterial plasmid DNA
2 recombinant plasmid inserted into bacteria
3 restriction enzyme cuts bacterial plasmid DNA
4 restriction enzyme cuts human DNA

What is the correct sequence for genetic engineering?​

Answers

Answer 1

Answer:

4, 3, 1, 2

Explanation:

FIRST: cDNA is synthesized to be used as a gene of interest: processed (spliced) mRNA (no introns) is reverse transcribed to cDNA using reverse transcriptase, an enzyme from retroviruses

SECOND: Restriction enzyme used to cut GOI

THIRD: Same Restriction enzyme used to cut plasmid (sticky ends are created)

FOURTH: GOI connects/hybridizes with plasmid due to base-pairing

FIFTH: DNA ligase connects the recombinant DNA strands


Related Questions

A farmer decides to try to increase his profits by mating his two goats that produce the most milk together. The farmer’s descendants continue the process and over 400 years the family produces goats that make more milk than any in the country. Eventually the goats change enough that they are designated a new species. This is an example of:

A. Artificial selection
B. Acquired characteristics
C. Disruptive selection
D. Natural selection

Answers

D. Natural Selection

Answer:

A.is the correct answer

Explanation:

People have been doing asimple form genetic enineeringfor thousands of years. This is called selective breeding. Selective breeding, or artifical selection, is a process where people try and improve plants and animals by selecting and breeding those that have desireable charactistics.

For example, a farmer might choose from his two largest cattle in his herd and breed them  together so that the offspring will be even bigger and produce more meat.

1. White blood cells attack what??
2. Ability of an organism to feed is called
a nutrition 6 irritability cd growth ad movement
3. The following are types of living things
except
Polista
d) lysosomea
4. Another name for Aues is called a hea
6) birds cd mamals d)
frog
animals are herbivores except
goats giraffes Selephants
d leopardo
5. The following
6. The locomotany organ of earthwor mis a) fors
Dwings as muscles D tube feet.
Sol
7. Spider use for respiration ad lungs b) booklungs
c) gills d) lenticels
The top of the tongue is responsible for a
bitterness c) Saltiness d) sourness.
these is not
organ
b) cose c) Tongue ad liver
9. One of
sense o
a) Eye​

Answers

Answer:

white blood cell's fight infections from bacteria, viruses, fungi, and other pathogens

Explanation:

which of the following are human activities that can negatively impact ecosystems.

Answers

Answer:

Hey not sure if there is something that is supposed to be viewable but some common human activities that can impact ecosystems are, deforestaion, pollution, littering, fracking, spilling oil into the oceans.

Explanation:

Answer:

You didn't put a photo but cutting down trees of putting chemicals into the water/earth can harm ecosystems

A class was performing an experiment to discover the pH values of some common household substances. The results of three groups are shown in the table below.

Substance
Group 1 pH
Group 2 pH
Group 3 pH
Lemon juice
2.2
2.2
2.1
Vinegar
2.8
2.8
8.2
Baking soda
8.4
8.5
8.4
Milk of magnesia
10.2
10.3
10.2

Which statement best describes evidence that Group 3’s data are less valid than the data for the other two groups?
a. Group 3’s pH value for lemon juice is lower than the values of the other two groups.
b. Group 3’s pH value for vinegar is much higher than the values of the other two groups.
c. Group 3’s pH value for baking soda does not exactly match the values of the other two groups.
d. Group 3’s pH value for milk of magnesia is different from the value obtained by Group 2.

Answers

Answer:

b. Group 3’s pH value for vinegar is much higher than the values of the other two groups.

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Answer:

B

Explanation:

I need help again, sorry

Answers

15,000 Kcal of energy will be transferred from green plants to rabbits
1,500 Kcal of energy will be transferred from rabbits to weasels
150 Kcal of energy will be transferred from weasels to eagles

What do you mean by clean water?

Answers

Answer:

it means water not polluted, without dangerous bacteria, trash and something like this

Clean water is unpolluted, water without bacteria and is not moggy, so I concur with the other person

I have to write a hypothesis based on if plants need sunlight
I would put one plant inside and one outside with everything they get the same except the amount of sunlight

Answers

Answer:

Sunlight is related to plant growth. If the amount of sunlight is increased, then plant growth will increase. The hypothesis that plant growth increases as the amount of sunlight increases was supported by the data.

Which of the following statements support(s) the theory of the endosymbiotic origin of chloroplasts?


Chloroplasts have their own DNA, separate from the DNA in the nucleus.
They contain their own ribosomes to translate and make proteins.
They self-replicate and reproduce on their own.
They don't have similarities to photosynthetic prokaryotes.
I only
I and II only
I,II, and III
I, II, and IV

Answers

Answer:

C

Explanation:

According to the endosymbiotic idea, a few eukaryotic cells' organelles were previously prokaryotic microorganisms. Chloroplasts and mitochondria divide by binary fission.  Therefore, option (C) is correct.

What is Endosymbiotic Theory ?

According to the endosymbiotic theory, eukaryote cell organelles like chloroplasts and mitochondria descended from separate bacteria that eventually developed symbiotic relationships with other cells.

Both mitochondria and chloroplasts have circular, single-stranded DNA in addition to not having histones. Prokaryotes are the only organisms that have single-stranded, circular DNA. They contain their own ribosomes to translate and make proteins. These qualities would enable the mitochondria and chloroplasts to thrive on their own, supporting the endosymbiosis theory.

Therefore, option (C) supports the theory of the endosymbiotic origin of chloroplasts.

Learn more about endosymbiotic theory, here:

https://brainly.com/question/28498218

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brain is considered as the centre of all senses, why ?

Answers

Answer:

The answer is

Explanation:

Human brain is considered as the centre of all senses as it can control what we can do. Without our brains, we can't walk, hold things or even move a pencil. A brain has different parts which perform different functions in the body. Brain is considered to be the centre of all senses because of these following reasons.

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The human mind is the command center for the human anxious device. It receives signals from the body's sensory organs and outputs records to the muscle tissues.

wherein are senses located in the brain?

Parietal lobe

It figures out the messages you get hold of from the 5 senses of sight, touch, odor, hearing, and taste. This part of the mind tells you what's a part of the frame and what is a part of the outside international.

Why is your mind vital?

The brain is arguably the most important organ in the human frame. It controls and coordinates moves and reactions, allows us to suppose and feel, and allows us to have reminiscences and feelings—all the matters that make us human.

Learn more about The human brain here: https://brainly.com/question/1247675

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Defining Populations

List three scales at which populations can be defined, from largest to smallest. Population groups​

Answers

From largest to smallest: biosphere, biome, ecosystem, community, population, and organism.

Carbohydrates info
A. AKA:
B. Composed of:
C. Monomer:
D. Polymer:
E. Functions:

Answers

A: Carbs
B:Carbon, hydrogen and oxygen
C:Monosaccharides
D:Polysaccharides
E: Provides energy to the human body.

A poison placed on the tips of darts by natives of the Amazon rainforest paralyzes prey such as small mammals. Which of the prey's cells are most likely affected by the poison?
muscle cells
epithelial cells
red blood cells
white blood cells

Answers

Answer:

Muscle cells

Explanation:

If they are paralyzed it stops use of the muscles. like... just read it bro

The cells in the prey that are most likely influenced by the poison present in the darts exist the muscle cells.

What are muscle cells?

A muscle cell exists also known as a myocyte when directed to either a cardiac muscle cell or a smooth muscle cell as these exist both small cells. A skeletal muscle cell exists long and threadlike with many nuclei and is named a muscle fiber. Muscle cells originate from embryonic precursor cells named myoblasts.

The muscle cells exist as a specialized kind of cell (one of the four tissues) that can contract to exert action.

These cells (muscle cells) can be paralyzed by utilizing various inhibitors of movement such as poisons.

In conclusion, the cells in the prey that exist most likely affected by the poison present in the darts exist the muscle cells.

The cells in the prey that exist most likely influenced by the poison present in the darts exist the muscle cells.

To learn more about muscle cells refer to:

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evolution by natural selection is an example of a scientific

Answers

Answer:

Ideas aimed at explaining how organisms change, or evolve, over time date back to Anaximander of Miletus, a Greek philosopher who lived in the 500s B.C.E. Noting that human babies are born helpless, Anaximander speculated that humans must have descended from some other type of creature whose young could survive without any help. He concluded that those ancestors must be fish, since fish hatch from eggs and immediately begin living with no help from their parents. From this reasoning, he proposed that all life began in the sea.

Have no idea hopefully some1 can help you

ASAP
When you become too cold, your hypothalamus will signal what parts of your body to raise the temperature?
A. Muscles and kidneys
B. Skin and pancreas
C. Muscles and skin
D. Kidneys and lungs

Answers

Answer:

c

Explanation:

Muscles and kidneys or muscles and skin

how is the cell membrane adapted to it's function

Answers

Cell membrane is made up of Lipid Bilayer which are fluid in nature , means they move continuosly , due to this fluid nature they are selectively permeable to compounds.

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Question
Use the text to identify the sentences with evidence that matches the claim.

Claim 1 --> Tropical rainforests contain more biodiversity than all other biomes.

1) Biodiversity refers to the total number of different species of all kinds living in an area.

2) Tropical rainforests have many of the climate characteristics that organisms of different species need to grow, such as warm temperatures, plenty of rain, and many food sources.

3) There are large amounts of daily sunlight, which provides a great deal of energy to a variety of rainforest organisms.

4) Surprisingly, rain forest soil is very poor.

5) Due to the large amounts of rainfall, nutrients are leached out of the soil quickly.

6) Warm temperatures cause dead organisms to decay quickly, and the nutrients are then used by many different plants.


1, 2, 5
1, 2, 5

1, 3, 4
1, 3, 4

2, 3, 6
2, 3, 6

1, 4, 5

Answers

Answer:

44

Explanation:

44-7=55

What is the effect of mutated GLMN Gene on the human body?

Answers

Answer:

A novel gene , glomulin (GLMN) in which 40 different mutation.

What are the three examples to justify Newton first law of motion .​

Answers

1. A book sitting on top of a table
2. A soccer ball rolling on the ground
3. A person driving a car to a stop

PLEASE ANSWER ASAP!!!!!!!! ITS DUE IN 5 MINUTES.

1.) How do organisms benefit from Mitosis? Write a paragraph using at least five sentences.


Answers

Answer:

Organisms benefit from mitosis because mitosis helps regenerate cells. This helps them recover from injuries and more.

Explanation:

how do you think the weight and the lift should not be of the same magnitude​

Answers

Answer:

There is because one is short and one is long

3. Calcium carbonate is the majour component of marble. Sulphuric acid one of the majour components of acid rain. Write a balanced chemical quation to show how the corrosion of marble statue by the acid rain.

Answers

Answer:

here

Explanation:

CaCO3 + H2SO4

-----> CaSO4 + H2O + CO2

What is a pedigree? Please choose the correct answer from the following choices, and then select the submit answer button.
a) a tool to document harmful traits only
b) a type of family tree that can help answer questions about genes and their inheritance patterns
c) a cross between animals other than humans
d) a type of genetic test to determine probability of inheritance
e) a way of following genotypes that are the same as the phenotype

Answers

Answer:

b

Explanation:

A pedigree is a genetic representation of a family tree that diagrams the inheritance of a trait or disease though several generations

Answer:

B

Explanation:

ANP is a hormone that causes:_________ a. constriction of the afferent arterioles and inhibition of renin release. b. dilation of the afferent arterioles and inhibition of renin release. c. dilation of the afferent arterioles and release of renin. d. constriction of the afferent arterioles and release of renin.

Answers

Answer:

b. dilation of the afferent arterioles and inhibition of renin release.

Explanation:

Atrial Natiuretic Peptide is a hormone which is mainly made and secreted in the atria. It helps in the regulation of blood pressure levels with the use of the kidney through an increase in water and sodium excretion.

The hormone helps in the regulation of blood pressure by ensuring there are dilation of the afferent arterioles to give room for increased flow of blood due to the increased pressure. Study also shows that the enzyme inhibits the release of renin.

what are the major groups of animals and how to they differ

Answers

There are many living things in the world. To keep them simple and easier to remember, the scientists had identify many groups of animals. The six main groups are: invertebrates, mammals, birds, amphibians, reptiles and fish.

Functions of the organs of amoeba​

Answers

Answer:

nucleus – the major organelle of the amoeba, located centrally; it controls reproduction (it contains the chromosomes) and many other important functions (including eating and growth). pseudopods – temporary “feet” that the amoeba uses to move around and to engulf food.

Explanation:

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When human skin suffers a cut, the process of healing rapidly begins allowing for wound closure and healing within a few days. Keloids occur when skin around wounds continues to grow after the skin has healed. A disruption in the regulation of which cellular process is probably responsible for this condition

Answers

Options

A. Mitosis

B. Meiosis

C. Apoptosis

D. Phagocytosis

Answer:

A

Explanation:

Mitosis is a type of cell division which takes place in living  organism during growth and development.Therefore it is ensures  the regenerations of cells and tissues during healing.

Therefore the restoration of new cells to the skin following injuries is due to mitosis.Since this is a multiplication division(2n) in which the daughter cells are exactly like the parents' cells the new tissues of the skin by mitosis, look exactly like the previous one that was injured.

In cases where the mitotic growth control is lost, the scare tissues of the injured part overgrows with granulation tissues and this leads to Kaloids.

      Its is a mass of Collagen Type 1.(Collagen is an  fibrous proteins  which has largest proportion in mammals).

Keloids  is characterized with pink or red coloration and elevation of the area,excessive growth of the area, with irritating  patch skin

A _____ country has high population density.

A: Densley populated country
B: Sparsely populated country
C: Open country
D: Overpopulated country

Answers

Answer:

A. A Densley populated country.

Explanation:

Need help anyone knows the answer

Answers

Answer:

landfill materials

besause it seems like the image show n

Scientific question that could be answered with the help of a microscope

Answers

Answer:

Size and structure of microbes.

Explanation:

The scientific question about size and structure of microbes could be answered with the help of a microscope because microscope help us to see very small objects such as bacteria and viruses etc which can not be seen without the help of microscope. With the help of microscope we can measure the size and see the structure of different microbes. So the questions about size and structure could be answered with the help of microscope.

why do solids have a fixed shape

Answers

Answer: solids have fixed shape because they are rigid and is high dense. The particles are way too close to each other that even force applied to it doesn’t change it shape

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