If bacteria transformed with a recombinant pUC18 plasmid produce white colonies when grown on plates containing ampicillin and Xgal, which of the following is least likely?

a. They produce β-lactamase that provides resistance to ampicillin.
b. They carry a vector that contains a DNA fragment inserted into the multiple cloning site.
c. They produce functional β-galactosidase that cleaves X-gal.
d. They carry a vector with a lacZ gene that has been disrupted and rendered nonfunctional.
e. They carry a vector with the bla gene used as the selectable marker.

Answers

Answer 1

Answer:

c. They produce functional β-galactosidase that cleaves X-gal.

Explanation:

β-galactosidase is an enzyme capable of catalyzing the enzymatic hydrolysis of β-galactosides into monosaccharides by hydrolyzing the β-glycosidic bond. On the other hand, X-gal is an analog of lactose composed of galactose, which is widely used in laboratories for the detection of β-galactosidase. In the presence of β-galactosidase, X-gal is hydrolyzed by cleaving its β-glycosidic bond, thereby confirming the presence of β-galactosidase.


Related Questions

3. How would you describe the waxing versus the waning portion of the moon’s journey

Answers

Answer:

A lunar eclipse would occur when the Earth is between the sun and moon. ... 3How would you describe the waxing versus the waning portion of the moon's journey? The waxing is when the moon is “growing”, while the waning is when the moon is “shrinking”.

The four principal types of stress are __________.

A. frustration, conflict, pressure, and anxiety
B. frustration, conflict, pressure, and change
C. anger, anxiety, depression, and annoyance
D. frustration, conflict, depression, and annoyance

Answers

Answer:

A

Explanation:

frustration, conflict, pressure, and anxiety

The four principal types of stress are frustration, conflict, pressure, and anxiety.

What is Stress?

This is characterized by a feeling of emotional or physical tension in individuals.

There are four types of stress which are listed above thereby making option A the most appropriate choice.

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Activation of the parasympathetic branch of the autonomic nervous system:______

a. enhances digestion.
b. causes conversion of glycogen to glucose.
c. triggers release of epinephrine.
d. increases heart rate.

Answers

Answer:

A - enhances digestion

The period of female fertility during each Month is short For fextilization to take place intercouce must occur each
a) A few hours before ovalation up to 4-5 days after avulation
b) Around 24 hours before ovulation up to around. 24 hours after ovulation
C) Sometimes between 2-3 days before ovulation upto 2-3 days after ovulation
d) Around 4-5 days before ovulation upto a few hours after ovulation
E) Around 4-5 days before ovulation upto 4-5. after ovulation.

Answers

Answer:

The so called fertility window is the days in the menstrual cycle when conception is ... Ovulation usually occurs 14 days prior to your period starting.

Explanation:

It isn't any of your selections but I hope it helped for the choices to decide which one would go to the paragraph I have given

Terminologies used in cattle

Answers

Answer:

Like.. naming types of cattle?

Explanation:

Names for cattle ? or names.. for things used on cattle, or things done to cattle... elaborate and i can help!!!

What percentage of the nation’s demand for electricity could be produced in the future by wind energy?

Answers

Answer:

5-10%

hope this is helpful

If a mother is affected by an X-linked dominant condition and the father is not, which children can inherit the condition?
only males
neither males nor females
both males and females
only females

Answers

Answer:

both males and females

Explanation:

The sex chromosomes in male and female humans are X and Y chromosomes. X-linked traits are those traits which are associated with the X- chromosome.

According to the question, the trait is passed on a X-linked dominant condition, which means that the possession or not of the condition is dependent on the presence of the dominant X-chromosome. The dominant X-chromosome will always express itself even when in an heterozygous state with a normal X-chromosome i.e Xx. Hence, only a individual recessive for the X-chromosome will be normal.

A mother that is affected by the X-dominant condition will either possess a; XX or Xx genotype while a father that is normal (not affected) will possess a (xY) genotype. Since the mother passes one of her X chromosomes to her sons and the other to her daughters, both male and female children will be affected by the condition.

N.B: If the mother is heterozygous for the affected X-chromosome (Xx), half of her sons and half of her daughters will inherit the condition but if the mother contains two dominant X-chromosomes (XX), all of her sons and daughters will inherit the condition.

What. Is black i will mark as brinelist ​

Answers

Just black?
Well it’s a colour which results from the absence of visible light. In technical sense black and white aren’t colour, but shades however, many people disagree since you combine other pigments to get black. Black is also associated with darkness, evil, despair and night (if it’s Literature related). It’s the darkest shade and it’s an achromatic colour.

simple. black. Is a color and it is dark

contrast primary growth and secondary growth

Answers

Answer:

There are contrast of the Primary growth and Secondary growth in many ways.

Explanation:

The Primary growth is to increase the length of that shoot and the root is referred to the primary growth.The primary growth act the way as shoot apical causing extension to the growth system into the root and ground they root are apical.The primary growth is the plant into that ground to contain water and nutrients that with the soil relation.The primary growth is that root can take different forms depends on the plant is Mono cot,the primary root replace by the stem after the plant germinates its called Adventitious root.The secondary growth is that performed to increase by the girth of the plant.Secondary growth is to produce the lateral cambiums that layer of tissue add to the plant growth.Secondary growth is that performed and support from the shoot system into the transporting  that water and nutrients.Secondary growth is to provide the cross section of the stem and the replace with newer layer, the layer of that growth bark.Secondary growth is to consist that bark remain that the narrow of band, it root is transformed.

Mutations in which of the following cells would get passed on to the next generation?
I. sperm
II. Skin cell
III. Egg
IV. Neuron
A. II, III, and IV
B. III only
C.I and III
D. I, III, and IV

Answers

Answer:

C. I and III

Explanation:

A mutation needs to occur in a gamete cell in order to be passed down to the next generation.

If it occurs in a somatic (body) cell, the mutation will only be present in that organism.

On the other hand, in a gamete cell, the mutation will pass down because gamete cells are directly involved in the genetic makeup of the offspring.

So, since sperm and egg cells are gametes, mutations in those cells would get passed on.

The correct answer is C. I and III

What are some functions of a cell membrane? Select all that apply.

The cell membrane shields the cell’s internal parts.

The cell membrane prevents the environment inside the cell from changing.

The cell membrane contains the cell’s DNA and RNA.

The cell membrane allows needed materials to enter

Answers

Answer:Only the 3rd statement is WRONG.

DNA and RNA are present in the nucleus of the cell

Explanation:THE CORRECT STATEMENTS ARE :

The cell membrane shields the cell’s internal parts.

The cell membrane prevents the environment inside the cell from changing.

The cell membrane allows needed materials to enter

The correct statements about the functions of a cell membrane are:

A. The cell membrane shields the cell’s internal parts.

B. The cell membrane prevents the environment inside the cell from changing.

D. The cell membrane allows needed materials to enter.

A cell is defined as the basic functional, structural and smallest unit of life in all living organisms.

Cell organelles refers to the inner organs (workers) that are responsible for performing various tasks or functions, especially for the survival of a living organism.

Basically, some cell organelles found in living organisms includes:

Nucleus. Cytoplasm.Golgi apparatus. Mitochondria.Cell membrane.

A cell membrane can be defined as the wall of a cell which typically controls what enters and leaves the cell.

Hence, some of the functions of a cell membrane are:

The cell membrane allows materials needed by a living organism to enter the cell.The cell membrane serves as a shield (wall) for the cell’s internal parts.The cell membrane preserves the environment inside the cell by preventing it from changing.

Read more: https://brainly.com/question/19559847

Why do you think premenopausal women need more iron than
men of the same age?

Answers

Answer:

Women need more iron than men to make up for the amount of iron they lose in their menstrual period.

Explanation:

Answer:

Premenopausal women shed blood as part of menstruation every month, which lowers the level of iron in the body. So, they need more iron than men and are also at a greater risk for this nutritional deficiency.

PLATO

Explanation:

Different between sinking and floating ​

Answers

sinking is being below and floating is being above or on surface

Answer:

⬇ See below ⬇

Explanation:

Sinking is where an object/human/animal goes under a type of surface, normally water. Floating, on the other hand, is where an object is at the surface of a liquid, normally water. (Hope this helps!)

What alternate form of genes do nucleic acids have that allows them to offer variability?

Answers

Answer:

allales

Explanation:

an allele is the variant from a gene

Answer:

A. allales

Explanation:

Explain what, if any, is the issue facing DNA polymerase in regards to its 5’->3’ activity when replicating DNA.

Answers

Answer:

The two strands of DNA are replicated in different ways

Explanation:

DNA replication is a process that occurs during the S phase of the cell cycle that consists of making two identical copies of the double-stranded DNA molecule, which subsequently are distributed in the daughter cells during cell division. During this process, DNA polymerase can add nucleotides in 5' to 3' direction, but not in 3' to 5' direction. In consequence, the DNA strand that has 3’ to 5’ directionality can be synthesized directly, while the DNA template strand that has 5’ to 3’ directionality can't be synthesized in a continuous manner and thereby it is created by adding small DNA fragments, which are known as Okazaki fragments (150-200 nucleotides in size).

Please I really need your help.

Answers

It is B because it exactly explained part of a population

What is the chain of custody?
A) the order of who gets custody of the crime scene
B) the paper trail of every person that has been in contact with the evidence throughout its seizure, collection, preservation, analysis and
disposition
C) the order of suspects
D) the people who have custody of children until the case is closed


Answers

The answer is b, the paper trail of every person that has been in contact with the evidence route and seizure collection preservation analyze and it depart

#1 Multiple choice: A. The highest temperatures are on land B. High ocean temperatures form a band in the tropics and subtropics that does not everywhere follow the equator C. Temperatures generally are higher on land than over adjacent oceans D. All of these are valid

Answers

Answer:

d all of these are valid generalizations

You classified organisms based on anatomical structure and development. Scientists also use DNA to classify organisms. Considering the basic process of evolution, why do you think it’s important to also consider DNA and gene patterns during the classification of organisms?

Answers

Answer:

By studying genetics and DNA, we can get the best idea of how species are related to each other, and therefore how to classify them. The genetic code of a species is not fixed, however. It changes over time! This is why we also need to factor in evolution.

It is important to also consider DNA and gene patterns during the classification of organisms because,

Through genetics and DNA study, we can get the idea of how species are related to each other and how to classify them. The genetic code of a species is not fixed. So, it need in evolution.

How organism classified ?The organism classified on the basis of shared characteristics.The classification of living organism includes seven levels, kingdom, phylum, classes, order, families, genus and species.

What is evolution?It is witness in the living species and change in heritable traits of biological population over successive generation.The process of development of complex organism from simpler one with time is called an evolution.

learn about gene,

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Why are G proteins known as guanine nucleotide-binding protein?

Answers

Answer:

G proteins, also known as guanine nucleotide-binding proteins, are a family of proteins that act as molecular switches inside cells, and are involved in transmitting signals from a variety of stimuli outside a cell to its interior. ... G proteins belong to the larger group of enzymes called GTPases.

Jeremiah is an 10-year-old male who is short in stature. His parents have become concerned as Jeremiah is now wetting the bed, drinking a lot of water, and often appears tired/fatigued. The doctor orders several blood tests after Jeremiah's urine test was positive for glucose and ketones.1. What disease(s) should be ruled out in this patient?2. List the tests that are used to diagnose diabetes.3. List the symptoms of diabetes.

Answers

Answer: 2) Tests used to diagnoses diabetes: Oral Glucose Tolerance Test, Fasting Glucose Test, Hemaglobin A1c, Random Blood Suger Test.

3) unexplained weight loss, frequent unination, lethargic, excessive thirst, nausea , and vomititng, increased hunger even though is still eating, blurry vision, bed-wetting in childern who did not do it previously, dry mouth,

Explanation:

which particles is the fundamental unit of all matter in both living and nonliving

Answers

Answer:

atoms

Explanation:

all things in the universe are made of atoms and they are considered the fundamental unit of matter.

non-living things are composed of different compounds and molecules.

living things are made of cells, and cells themselves are made of different molecules. So living things are also made of atoms.

What are similarities and differences between insects that go through complete metamorphosis and incomplete metamorphosis?

Answers

Answer:

There are two types of metamorphosis-incomplete and complete.

In incomplete metamorphosis, an adult insect lays an egg. The egg hatches into a nymph, which is like a mini adult. This nymph then grows into an adult. As the nymph grows, it molts several times. Grasshoppers are examples of insects that undergo incomplete metamorphosis.

Explanation:

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g In humans and higher primates, _____ is the final product of purine base catabolism and is excreted in urine. However, its inadequate excretion is a cause of a disease called ______

Answers

Answer: Humans and greater primates excrete water, broken down proteins and Urea. Many things cause unusual urine, Urea gives urine its yellow color a lack of urea can mean kidney failure. however it's hard to tell a lack of urea by just looking at urine as drinking excessive amounts of water turns the color clear and drinking caffeine turns the color reddish or brown so lab testing must be done

According to kirk smith a professor of environmental health at the university of california berkley indoor fires increase risks of pneumonia tuberculosis lung cancer and low birth weight in babies born of women expose during pregnancy. What simple solution is being widely promoted to reduce the risk of death?
a) preparing meals using solar cookers.
b) switching from wood to burning crop waste as a fuel source.
c) adding more windows to houses as a source of ventilation.
d) passing a green tax to make homeowners pay for their pollution.
e) providing asthma inhalers to children under the age of 12 years.

Answers

Answer:

preparing meals using solar cookers.

Explanation:

solar cookers  radiates at low rates.Therefore the most of the nutrients in the foods are conserved.Thus most micro nutrients for biochemical activities are retained. Vitamins which can not withstand high temperature are preserved.

Most importantly carcinogens which are usually associated with high heat foods are avoided ,when cook with low heat of solar cookers

The solar cooker is smoke free,therefore irritation of the lungs,lung cancer associated with high heat  cookers is avoided.

Specifically,local Mayan women exposed to high heat smoke cookers, suffers lung cancer,and those with pregnancy gives to infants with low birth weights. Children exposed to theses high heat also experienced acute lower respiratory infections.

Thus the smokeless,low heat solar cooker is safer.

assuming hardy weinberg equilibrium what is the probability that sarah who is phenotypically normal with no family history and tom

Answers

Answer:

The probability of having a disorder is 50 %.

Explanation:

The offspring has that genetic disorder if the Sarah who is phenotypically normal with no family history and tom has a genetic disorder so there is 50 percent chance that the children of Sarah and tom has that genetic disorder. if the disorder linked with Y chromosome of male so this disorder is present in the sons but if the disorder linked with X chromosome of male so this disorder is present in the daughters.

In the quest to understand the basis of infertility in humans, researchers have identified a mutation in a gene associated with chiasmata. This protein normally acts to promote homologous recombination.Why might a defect in homologous recombination have consequences for fertility?A. The chiasmata halts the whole process of meiosis, if crossover do not form properly.B. Crossover formation is a necessary step in meiosis I to ensure proper chromosome segregationC. A checkpoint requires a certain level of genetic variability for meiosis to proceed.D. Chiasmata are the connections between the centromeres and the centromeres that pull them to each pole of the daughter cells.

Answers

Answer:

B. Crossover formation is a necessary step in meiosis I to ensure proper chromosome segregation

Explanation:

Crossing-over is a unique phenomenon that occurs in the prophase I stage of meiosis I, where non-sister chromatids of homologous chromosomes exchange their chromosomal segment. The physical point where this exchange occurs is called CHIASMATA. Hence, a mutation that affects the gene associated with the chiasmata will affect the occurrence of crossing over or homologous recombination.

Crossing-over, through the formation of the chiasmata, is responsible for the physical alignment and proper segregation of chromosomes into gametes. Naturally, the chiasmata formed as a result of recombination during meiosis helps ensure that the chromosomes stay together until it is the right time to separate. This way, any chromosomal defect in the resulting gamete is prevented.

However, an error or defect in homologous recombination might give rise to gametes with chromosomal disorder, a condition known as ANEUPLOIDY i.e. missing or additional chromosomes in gametes. This can affect the fertility of the involved human.

Describe the intermolecular interaction of the surface carboxylate groups of a micelle and the surrounding water.

Answers

Answer:

Hydrophilic portions are outside and hydrophobic regions are inside

Explanation:

A micelle is an assembly of amphiphilic molecules formed in a liquid solution, generally an aqueous solution. Micelles are formed due to the packing (aggregation-like) behavior in which single-tail lipids are organized in a bilayer. A typical micelle in aqueous solution forms a spheric assembly where carboxylate ions are organized in a mode that hydrophilic head regions enter in contact with the surrounding water, while hydrophobic (lipophilic) portions are disposed inside the micelle.

How do vaccines act on germs?

Answers

Answer:

a vacine work by traning the immune system by reconize and combine either virus or bacteria.

Where does that air go as a result?

Answers

Answer:

Air go from one place to another due to difference in temperature of land and sea.

Explanation:

Air go as a result of wind from one place to another. Air moves from land to sea is called land breeze and from sea to land is known as sea breeze. This will occur when there is difference in temperature between sea and land. Sea breeze occurs at afternoon while land breeze occurs at night till morning. Warm air produces low pressure and cold air produces high pressure so when warm air rises, cold air takes its place and this cycle continues.

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