If you subcultured a microbial colony by transferring some of the cells into a tube of sterile nutrient broth, would the new culture still be considered a pure culture?

Answers

Answer 1

1. Samples of a microbial colony obtained from the natural environment are not pure culture. They are mixed cultures because the environment consists of an array of microorganisms and not one specific organism. A sample obtained from the natural environment such as water or soil may contain several species of microbes that need to be further isolated to obtain a pure culture.  

2. Isolation streak plate technique involves the dilution of the sample several folds and then streaking a small amount using a sterile inoculation loop on an agar plate in a zig-zag manner. This is done to obtain a pure culture of bacteria from a mixed population by separating the microprocessor. When the sample is diluted into a small concentration and is streaked onto agar placed, widely spaced, discrete colonies of bacteria can be observed. These isolated colonies can be further cultured to obtain pure cultures by picking and re-streaking them on fresh agar plates.

3. A microbial colony is considered a pure culture because it consists of single species. A single species of bacteria cluster and form an isolated colony on an agar plate which can be further plated on fresh medium.

3-1 - If your agar plate shows discrete uncluttered colonies then you have obtained an isolated colony. The following picture might help you to understand.

3-2. If you subculture isolated microbes into a sterile nutrient broth without any contamination, it will still be considered pure culture because it is single isolated species in a sterile aseptic environment of the nutrient broth.

3.3. A pure culture can become a mixed culture due to contamination. This could be caused by airborne bacteria if your conditions are not aseptic. Contamination is possible if the work area is not clean and sterile or if the glassware is not sterile. The contamination can transform your pure culture into a mixed culture and is hence problematic. So, it is very important to maintain a sterile and aseptic environment, glassware, and instruments.

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Related Questions

Shannon’s maxim reflects which of the textbook’s basic principles?

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Shannon’s maxim reflects Open Design of the textbook’s basic principles.

Kerckhoffs's principle was reformulated by an American mathematician called Claude Shannon as "the enemy knows the system". The mathematician Claude Shannon further refined Kerckhoff’s principle.

According to Shannon’s maxim, "one ought to design systems under the assumption that the enemy will immediately gain full familiarity with them." Thus, in this form it is called Shannon's maxim.

Shannon is regarded as the father of the information age, as he formulated the notion of channel capacity in 1948 A.D. He is also known to reflect Open Design of the textbook’s basic principles.

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Based upon the amount of planning, three styles of robbery can be identified. these are the?

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These are ambush, selective raid, and planned operation.

What are the three main elements for the crime of robbery?

Possess a lethal weapon on one's person or near one; Cause, threaten, or attempt to cause bodily injury to another; Use or threaten to use imminent force against another.

Is it a style of robbery that involves no planning and depends almost entirely on the element of surprise?

The ambush is a type of robbery that requires no preparation and largely relies on surprise. The victim is alone, traveling to or from leisure activity, and less than five miles from their house in 93 percent of visible street robberies.

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________________ means using the language that adapts to the needs, interests, knowledge, and attitudes of the listeners, and avoiding language that alienates the audience.

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Hi there,

Speaking Appropriately means using the language that adapts to the needs, interests, knowledge, and attitudes of the listeners, and avoiding language that alienates the audience.

Please leave 5 stars and a like if you find this answer to be helpful!

Happy to help!

~Garebear

How can nlw promote coalition support?

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NLW can promote coalition support By providing a gradual response option, reducing collateral damage, and minimizing fatalities

According to the Bureau of Labor Statistics, 2.1 million US high school students who graduated between January and October 2019 entered college shortly after graduation. To enroll in college, future students often have to apply to multiple schools, which requires the submission of college essays, testimonials, and standardized test scores.

The Common App and Coalition App allow students to fill out standardized applications and apply to multiple universities.

The next section describes simultaneous and collaborative apps and provides tips to help you decide which app to use when applying to college.

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A(n) __________ is an abstract set of behaviors, structured as methods, that are designed to provide data for a specific scenario

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A(n) abstract data type (ADT) is an abstract set of behaviors, structured as methods, that are designed to provide data for a specific scenario

More about abstract data type (ADT):

An abstract data type (ADT) is a mathematical representation of a data type in computer science. A data type is characterised by the behaviour (semantics) it exhibits when viewed from the perspective of a user, specifically in terms of the potential values, operations that may be performed on this type of data, and the behaviour of those actions. Data structures, which are physical representations of data and are viewed from the perspective of an implementer rather than a consumer, contrast this mathematical paradigm.

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A(n) abstract data type (ADT) is an abstract set of behaviors, structured as methods, that are designed to provide data for a specific scenario

More about abstract data type (ADT):

An abstract data type (ADT) is a mathematical representation of a data type in computer science. A data type is characterised by the behaviour (semantics) it exhibits when viewed from the perspective of a user, specifically in terms of the potential values, operations that may be performed on this type of data, and the behaviour of those actions. Data structures, which are physical representations of data and are viewed from the perspective of an implementer rather than a consumer, contrast this mathematical paradigm.

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Negative stereotypes about aging _____ the memory performance of older adults. Group of answer choices decrease confuse increase are unrelated to

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Negative stereotypes about aging are unrelated to the the memory performance of older adults.

What are negative stereotypes?

This is the term that is used to refer to the type of stereotype that is done to bring about an undesirable feeling in a person.

This talks about the characteristics that are not acceptable or have good desires to the member of the group.

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Maximilien robespierre, a jacobist, argued for and became a member of what body that formed a dictatorship led by a 12-person committee for public safety?]

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Maximilien Robespierre, a jacobist, argued for and became a member of Committee of Public Safety.

What was the Commitee of safety?

This was a committee that was headed by Robespierre. What the committee did at the time was to encourage the death oof thousands of people by the guillotine in France.

Those that were killed were tagged as the enemies of the revolution. This body was the political body of the French revolution.

The way that it ended was that he was also executed through the same menas in front of a cheering crowd.

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At lamb's peace church, the music director, youth group director and the director of religious education share the services of the administrative staff. this is an example of ______ interdependence.

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At lamb's peace church, the music director, youth group director and the director of religious education share the services of the administrative staff. this is an example of Pooled interdependence.

A task circumstance in which the task is divided among a number of people, units, or groups, each of whom executes independently with little to no contact or coordination needed and no flow of work between them.

The output of each person, group, or unit is eventually combined and helps the organization as a whole achieve its overall objectives.

For instance, each employee in a company's sales department works individually throughout the day to sell a certain product, and at the end of the day, the total number of sales made by all employees is added up to provide the company's overall sales statistic.

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richard a rogers From Cultural Exchange to Transculturation: A Review and Reconceptualization of Cultural Appropriation

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A Review and Reconceptualization of Cultural Appropriation:

INTERPRETATION-

Cultural Appropriation is often mentioned but undertheorized in critical rhetorical and media studies. Defined as the use of a culture’s symbols, artifacts, genres, rituals, or technologies by members of another culture.

Cultural Appropriation can be placed into 4 categories:

Exchange.Dominance. Exploitation. Transculturation.

Although each of these types can be understood as relevant to particular contexts or eras, transculturation questions the bounded and proprietary view of culture embedded in other types of appropriation.

Transculturation posits culture as a relational phenomenon constituted by acts of appropriation, not an entity that merely participates in appropriation. Tensions exist between the need to challenge essentialism and the use of essentialist notions such as ownership and degradation to criticize the exploitation of colonized cultures.

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Manufacturers can regain some control over the price their products are sold for at the retail level by:______

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Manufacturers can regain some control over the price their products are sold for at the retail level by providing quality and value, avoiding trading with discount, franchising, and increasing consumer brand loyalty by using an exclusive distribution system for retail products.

Manufacturers face their own pricing difficulties. The challenge is to accurately estimate the constantly changing products and the fair market price / value of those products. Manufacturers need to understand the different types of costs and their behavior. Up-to-date knowledge of market conditions is also essential. Best-selling prices should be cost- and market-oriented. It must be high enough to cover costs and make a profit.

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which sport-related concerns are frequently faced by athletes as they approach their return to participation?

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The status of the athlete and the game situation get affected and are faced by athletes as they approach their return to participation.

The Greek word athletes, which means "contestant at the games," is where the noun athlete originates. Anyone who participates in athletic tournaments can be referred to as an athlete, but this label is typically reserved for those with a high level of commitment, talent, and fitness.

The top athletes are naturally confident and genuinely think they will succeed. Additionally, they are self-assured enough to handle any unforeseen circumstances that may emerge and, when necessary, to speak out for themselves or their teammates.

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When looking at inequalities of wealth, more americans than ever are investing in the stock market and this has resulted in:_____.

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When looking at inequalities of wealth, more Americans than ever are investing in the stock market and this has resulted in more wealth inequality.

Several factors contribute to the rise in economic inequality in the United States. These include, in no particular order, technological change, globalization, the decline of unions, and the declining value of minimum wages.

Wealth inequality in America expanded tremendously from 1989 to 2016, with the top 10% of families measured by household wealth (net worth of at least $1.2 million) falling short of their share of the American “pie”. It has grown to 77%. property ownership. The bottom half of the families ranked by household wealth (those whose net worth is less than $97,000) own only 1%.

US household wealth holdings have been somewhat concentrated since the 1980s. The top 10% of households held 68.2% of total wealth in 1983 and 73.1% of total wealth in 2007.

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which is a characteristic of a person with positive mental well-being a. ability work productivity by. ability to play sports well c. ability to create beautiful art d, ability to play video games well

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The characteristic of a person with positive mental well-being a. the ability to work productivity.

Intellectual fitness is a kingdom of mental well-being that enables human beings to address the stresses of existence, recognize their talents, learn well and work properly, and make a contribution to their community.

Examples include desirable professional relationships, clear remarks and instructions, advantageous feedback, and work–existence balance. In comparison, elements that include bad or uncertain communique, unrealistic cut-off dates, negative relationships and place of work struggle, and a loss of aid can motivate terrible intellectual well-being.

Intellectual fitness includes our emotional, psychological, and social well-being. It influences how we assume, feel, and act. It additionally facilitates determining how we handle the strain, relate to others, and make healthy choices. Mental health is crucial at each degree of existence, from youth and adolescence to adulthood.

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Sasha asked people at the rally to sign her petition. Once they agreed to sign, Sasha then asked them to make a donation; an example of

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If Sasha asked people at the rally to sign her petition. Sasha then asked them to make a donation; this is an example of Foot-In-The-Door Technique.

What is Foot-In-The-Door Technique?

Foot-In-The-Door Technique can be defined as  a technique which purpose is to enable people to agree or accept  a larger  request by making  them to accept to a smaller request first.

Sasha is making use of Foot-In-The-Door Technique as their is tendency or likelihood that the people will most likely donate since they have already signed the petition.

Therefore this is an example of Foot-In-The-Door Technique.

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Psychologists discover the relationship between religious faith and good health. this is an example of __________ research.

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Psychologists discover the relationship between religious faith and good health. this is an example of cross sectional research.

What is cross sectional research?

Cross-sectional research can be defined as the type of research that is conducted by measuring, analyzing and evaluating the data or information collected before reaching a conclusion.

Discovering the relationship  between religious faith and good health is an example of what is called Cross-sectional research .

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Used widely by indian companies, __________ refers to an innovation mind-set that strives to meet customers' immediate needs quickly and inexpensively.

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Jugaad refers to an innovation mind-set that strives to meet customers' immediate needs quickly and inexpensively, which is used widely by Indian companies.

Jugaad essentially refers to a mindset or strategy that looks for answers in difficult situations, explaining how inventiveness and improvisation are used to get by in life. The term describes the act of defying convention and thinking outside the box to make something work.

Making or performing anything on the spot using whatever is available is known as improvisation. A performance that is improvised in the performing arts is one that is completely unplanned and unscripted.

It's increasingly necessary for many organizations since improvisation may encourage problem-solving, creativity, and innovation. Even though it may appear to be spontaneous and intuitive, improvisation involves the development of purposeful and disciplined processes and abilities in order to be done properly.

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When an australian hunter gives away most of his meat to relatives without specifying what is expected in return, he is exemplifying:____.
A) generalized reciprocity.B) negative reciprocity.C) redistribution.D) potlatch.E) market exchange.

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When an Australian hunter gives away most of his meat to relatives without specifying what is expected in return, he is exemplifying A) generalized reciprocity.

Generalized reciprocity is the phenomenon that individuals deal with others in the equal manner that others dealt with them in the past. besides the behavioral consequences, whether or not purpose facts additionally manipulate generalized reciprocal conduct stays unclear.

Generalized reciprocity is present giving without the expectancy of an immediate go back. For an instance, in case you are shopping with a pal and also you buy him a cup of coffee, you may assume him to buy you one in go back at a while in the destiny.

Generalized reciprocity refers to a kind of trade of goods and/or services wherein the giver and the recipient do not keep a specific ledger of cost or stipulate the amount or duration of going back.

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Jason has always been unsure of himself and his job skills, so when the boss gives him a new task, he experiences feelings of fear and anxiety that make him sure he won't succeed. Which determinant of self-efficacy does this represent?

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Emotional cues are the factor that determines self-efficacy that this represents.

Self-efficacy: What is it?

Self-efficacy is the conviction that one is capable of doing the actions required to accomplish particular objectives. It should be emphasized that Albert Bandura, a psychologist, was the one who first put up the idea.

Self-efficacy is the conviction that one has the power to exert control over one's own motivation, behavior, and social environment. It is the notion that one can carry out the behaviors required to accomplish particular performance attainments.

In this instance, Jason has always felt uncertain of himself and his abilities at work, so when the boss assigns him a new responsibility, he feels worried and anxiety and is confident he won't succeed. a factor that determines self-efficacy

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The processes of assimilation and accommodation were most clearly highlighted by?

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The processes of assimilation and accommodation were most clearly highlighted by Jean Piaget in his cognitive development theory.

Jean Piaget suggested in his cognitive development theory that intelligence changes as children grow. A child's cognitive development is not just about obtain knowledge, the child has to develop or construct a mental model of the world through which they can adapt and learn things.

Piaget divided children’s cognitive development in four stages, each of the stages represent a new way of thinking and understanding the world.

He called the First stage as sensorimotor intelligence, Second as preoperational thinking, Third as concrete operational thinking and Fourth stage is of formal operational thinking. Each stage is correlated with an age period of childhood, but only approximately.

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Which data role enables advanced analytics capabilities specifically through reports and visualizations?

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The data analyst role enables advanced analytics capabilities specifically through reports and visualizations.

Data analysis is the process of investigating, cleaning, transforming, and modeling data with the goal of discovering useful information, drawing conclusions, and supporting decision-making.

Data analysts review data to gain important insights into corporate customers and find ways to use the data to solve problems. We also share this information with senior management and other stakeholders.

Data analysts also do not need to have advanced programming skills. Instead, you need experience with analytics software, data visualization software, and data management programs. As with most data carriers, data analysts need to have high-quality math skills.

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What are the participations, what are the cardinalities, and under which circumstances would the ones depicted be correct or incorrect?

Answers

In the context of databases, cardinality refers to the uniqueness of data values contained in a column.

How does cardinality work?

The quantity of fundamental (cardinal) members of a set is referred to as cardinality. Both infinite and finite forms of cardinality exist. The cardinality of the set of individuals living in the United States, for instance, is roughly 270,000,000; the cardinality of the set of integers is incalculably limitless.

For databases with discrete values, high cardinality is appropriate. Consider the case where each line item has its own specific ID number, description, email address, etc. The term "low cardinality" refers to a column that has many repeated values. It's critical to evaluate your process before using it because different people calculate cardinality in different ways.

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42. Terry's home was destroyed in a hurricane. It is hard to find food, so he is constantly hungry. However, he feels lucky because his family survived and he is together with his loved ones. According to Maslow's theory, Terry's ____needs are not met, but his____needs are being met.

Answers

Terry's home was destroyed in a hurricane. It is hard to find food, so he is constantly hungry. However, he feels lucky because his family survived and he is together with his loved ones. According to Maslow's theory, Terry's physiological needs are not met, but his belonging needs are being met.

What are physiological needs?

Maslow's hierarchy of needs places physiological needs at the bottom. They are the most important things a person requires to survive. Shelter, water, food, warmth, rest, and health are among them. At this level, a person's motivation stems from their instinct to survive.

What are belonging needs?

Love and belonging needs are at the third level of Maslow's hierarchy of needs. Humans are social beings who seek interaction with others. The need for friendship, intimacy, family, and love is outlined at this level of the hierarchy. Humans have a need to give and receive love in order to feel like they are part of a group. Individuals who are deprived of these needs may experience loneliness or depression.

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the basic assumption of behavior therapy is that: faulty patterns of thinking are producing the current difficulties.

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the basic assumption of behavior therapy is that: adaptive and maladaptive behaviors are learned.

What is behavior therapy?

This is the term that is used to refer to the type of therapy that is intended to bring about a change in the behavior of an individual. This type of therapy becomes necessary in a person that is acting in ways that are unacceptable.

The goal is to help to reinforce positive attitude and behavior that are more acceptable in the society to the individual that is undergoing this type of therapy.

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A good strategy should be able to provide products and services to customers at an attractive price point while maintaining internal costs, resulting in:_______

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A good strategy should be able to provide products and services to customers at an attractive price point while maintaining internal costs, resulting in: Cost leadership strategy .

Cost leadership is a successful business approach when a company offers competitive pricing, delivers acceptable quality, and draws in enough consumers to be profitable.

Cost leaders frequently keep their expenses low by cutting back on marketing, market research, and R&D, but this strategy might wind up being costly over time. Cost leaders may be less adept than competing companies in spotting significant alterations and trends in the external environment due to a relative lack of market research. The capacity of cost leaders to react to changes once they are seen might be slowed considerably if research and development are undervalued. Cost leaders may find themselves out of touch with the market and at a loss for solutions if they lag behind competitors in spotting and responding to external trends.

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People whose genetic ancestry & family history convey membership in one racial category but who are perceived to belong to another race in the eyes of others, refers to which term?

Answers

People whose genetic ancestry & family history convey membership in one racial category but who are perceived to belong to another race in the eyes of others are been  referred to as  passing.

What is  genetic ancestry ?

Genetic ancestry  can be regarded as the  genetics concept which is been used in the explanation of the "architecture of genome variation that can be found in between populations.

It should be noted that with Genetic ancestry tests  the population genetics research  that can give inferences about someone's genetic heritage can be found.

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As a systems development team manager, you know that your team needs to be heavy in ________ during the requirements definition phase.

Answers

As a systems development team manager, you know that your team needs to be heavy in software testers during the requirements definition phase.

Who is a team manager?

The team manager leads a group of employees or team to success over a particular project.

It involves close monitoring of operations of a section of the company and projects. Team leader needs people who are vast in the area of given project to work on it.

Therefore, a system developer needs software tester that can designs test scenarios during definition phase of project for software usability.

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What two cities were the chief financial and commercial centers of the mid-atlantic colonies?

Answers

New York and Philadelphia two cities were the chief financial and commercial centers of the mid-Atlantic colonies.

Oglethorpe, like John Locks, had a vision for Georgia. He rejected the idea that a person is born in a healthy environment and cannot be rehabilitated.

They were commercial centers and financial centers. All the cities within the two states had thriving ports that facilitated trade between Europe and the rest of the coastal world. It was a cultural and geographical area where Native Americans and French traded goods with each other, and neither side could impose their will on the other by force.

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Even when you have the right-of-way, you're responsible for
before you
drive forward.

Answers

Even when you have the right-of-way, you're responsible C. making sure the path is clear you're responsible for before you drive forward.

What Are the Important Regulations of the Road?Always watch your surroundings when reversing.Slow traffic controls right.Signal before shifting or changing lanes.Keep a safe following length.Consistently yield to pedestrians.Stop after school buses with red flashing lights.What are the basic of driving?

Always preserve a safe speed and beware of blind spots and restricted areas. Following the rate limits is an important part of driving. The right speed can contain numerous mistakes. Be courteous on the road, and if you're going slowly than the flow, let other motorists pass

complete question is shown below

Even when you have the right-of-way, you're responsible for _____ before you drive forward.

A. waving to other road users

B. honking to alert other road users

C. making sure the path is clear

D. flashing your headlights

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The saanvi family wants to move to a new town. How can they best determine whether a certain part of the town has gangs?

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The Saanvi family wants to move to a new town. Assess current criminal reports in the area, by this they can determine whether a certain part of the town has gangs.

Gangs are organizations of kids, teenagers, and young adults who have a shared identity and engage in wrongdoing or delinquent behavior.

The majority of gang members are typically teenagers or young adults, but new statistics show that kids are joining gangs far earlier, sometimes when they are still in elementary school.

In the past, gang activity was only found in big cities, but today, gangs may also be found in smaller towns and rural areas. Every gender, color, culture, and socioeconomic group can be found in gangs.

Some young people are inspired to join a gang in order to feel connected or to create a new identity. Peer pressure drives certain people.

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True or false: understanding the relationship between problems and inputs and knowing how to create a strong problem statement are related to six sigma project success

Answers

It is True that understanding the relationship between problems and inputs and knowing how to create a strong problem statement are related to six sigma project success.

What is Six Sigma project ?

A Six Sigma Project is been used in a  project and bring about  enthusiastic support for the project.

However, it should be noted that  understanding the relationship between problems and inputs and knowing how to create a strong problem statement.

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The Beanny Company had budgeted sales of 1,000 units with a per unit selling price of $5 per unit. Actual sales were 1,100 units at a selling price of $4.75. What is its sales volume variance? A firm that successfully leverages network effects can? Afghanistans national government in the capital city of kabul has little influence in the countrys rural regions in large part due to the many __________ living there. Refer to Figure 7-5. If the supply curve is S', the demand curve is D, and the equilibrium price is $150, what is the producer surplus Expository: Explain the steps that a young person must take to be granted a drivers license. Be sure to include important details about each step, including the challenges that a person will face along the way and how to overcome them effectively. We're happy to say that the bad weather has not affected our hours, and your equipment will be ready on schedule. Write an absolute value inequality for each of the following. Then graph thesolution set on a number line. all numbers less than 7and greater than -7 Complete the square to write y = 3x2 + 12x + 7 in vertex form, y = a(x - h)2 + k. Which of the following statements is accurate about antidiuretic hormone (ADH) Jay's new loan to purchase a property includes the seller's existing mortgage. What type of loan is this All the students in a science class complete a 15-point extra-credit assignment to raise their test scores the new test score is 15 points more than the original score let x = original score let y = new score ehich equation represents this situation? Spending plans are divided into three categories with roughly ______% of the after tax budget going to the category of needs and _____________ % of the after tax budget going to wants, with the rest going to esi. bottom up approach is not applicable when Try itwriting a SummaryWith advancing years I feel I must give this question ofwhat I want to leave behind me greater thought, forbefore long I shall be moving on to fields unknown, andperhaps it may make a difference if I actually know whatI would like to bequeath to a new generation. Perhaps thebest I can do is to pray that the youth of today will havethe ability to live simply and to get joy out of living, thedesire to give of themselves and to make themselvesworthy of giving, and the strength to do without anythingwhich does not serve the interests of the brotherhood ofman. If I can bequeath these desires to my own children,it seems to me I will not have lived in vain.-"What I Hope to Leave Behind,"Eleanor RooseveltBased on the final paragraph and what you rememberfrom the speech overall, write three to four sentencessummarizing what Roosevelt hopes to leave behind. The swallowing center is located in the __________. How do the pill and mini-pill provide protection against pregnancy?O They kill sperm.O They block sperm from reaching an egg.O They prevent the ovaries from releasing an egg.They release progestin into the body to prevent fertilization. Solve for x.A) 6B) 4C) 5D) 7 The southern most tip of the main land of India is_____ The small spherical planet called "Glob" has a mass of 7.881018 kg and a radius of 6.32104 m. An astronaut on the surface of Glob throws a rock straight up. The rock reaches a maximum height of 1.44103 m, above the surface of the planet, before it falls back down. 1. What was the initial speed of the rock as it left the astronaut's hand? (Glob has no atmosphere, so no energy is lost to air friction. G = 6.6710-11 Nm2/kg2.)2. A 36.0 kg satellite is in a circular orbit with a radius of 1.45105 m around the planet Glob. Calculate the speed of the satellite. A machine in a candy company creates 9,328 pieces of candy each hour. if a box holds 98 pieces of candy, how many boxes can be filled in one hour?