It is often desirable to express eukaryotic genes in bacteria, which can make a protein of interest quickly and cheaply. What is TRUE about this kind of genetic transformation

Answers

Answer 1

The expressed gene must be incorporated into the bacterial chromosome, but its promoter region should be replaced with a bacterial promoter.

What about bacterial chromosome?The term "nucleoid" refers to a bacterial chromosome. It has genetic material inside and is a strange shape. It may also be known as a genophore.Both transcriptional activators and repressors control gene expression in eukaryotic cells. Eukaryotic repressors bind to specific DNA sequences and prevent transcription, just like their prokaryotic counterparts.Since transcription and translation are physically distinct processes, eukaryotic gene expression is more complex than prokaryotic gene expression. Eukaryotic cells, as opposed to prokaryotic cells, have a wide range of levels at which they can control gene expression.While transcription and translation can both happen at the same time in bacteria, in eukaryotes, transcription takes place in the nucleus and translation happens in the cytoplasm.

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Disclaimer: The question was given incomplete on the portal. Here is the complete question.

Question: It is often desirable to express eukaryotic genes in bacteria, which can make a protein of interest quickly and cheaply. What is TRUE about this kind of genetic transformation?

a. The expressed gene and the genes for all of its regulatory elements must be incorporated into the bacterial chromosome.

b. Only polycistronic genes are capable of being expressed in a bacteria.

c. The expressed gene and the gene for RNA Polymerase II must both be incorporated into the bacterial genome.

d. Only the transcribed region of the gene should be incorporated into the bacterial genome. No promoter is necessary to transcribe a transformed gene.

e. The expressed gene must be incorporated into the bacterial chromosome, but its promoter region should be replaced with a bacterial promoter.


Related Questions

Collecting ducts within the kidney tubules cut in cross section would reveal what type of epithelium?

Answers

Simple cuboid epithelium found in glandular kidney tubules. This is observed in the lining of the kidney tubules and ducts of glans. Nucleus of tall column like cells trend to be elongated and found in the basal end of the cells.

It is composed of single layer that is relatively ,larger surface area, which is allow them to absorption and secretion of substances like water and salt from the kidney and hormones which are produced by the glands.

Simple epithelium tissues are specialized for diffusion and absorption. This is also found in lining the cavities of body such as pericardial , peritoneal and pleurae.

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What type of container is required for transporting low-level radioactive materials, such as protective clothing?

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Any shipment of radioactive materials, whether from the government or the private sector, must be carried and packaged in accordance with stringent Federal requirements. These restrictions shield the public, transportation employees, and the environment from radiation exposure.

Adhering to the necessary packing regulations can limit the danger of transporting radioactive items most effectively.Based on these considerations, radioactive material is transported in one of three different types of containers.

Type A packagingType B packagingIndustrial packaging

Due to their low radioactivity, industrial packages are used to convey materials that pose a little risk from radiation exposure. These are also referred to as sturdy, secure containers. This container will keep its contents and protect them during routine transportation operations. Materials that might be transported in industrial packaging include smoke detectors, lab samples, and slightly contaminated clothing.

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What is the second greatest color of light chlorophyll A absorbs?

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Answer: Chlorophyll a absorbs violet and orange light the most. Chlorophyll b absorbs mostly blue and yellow light.

Explanation: They both also absorb light of other wavelengths with less intensity.

What is the coarse adjustment for in a microscope?

Answers

Answer:

Answer below

Explanation:

A rapid control which allows for quick focusing by moving the objective lens or stage up and down. It is used for initial focusing.

Hope this helped and have a good day

_____________ is the idea that an organ or device that evolved for one purpose was available to be used for another purpose.

Answers

Explanation:

the evolution is the idea that an organ or device that evolved for one purpose was available to be used for Another purpose

Why is the immune system necessary in
an organism?
A. without it there would be nothing to fight off infection
B. it provides structure for the body
C. the cells transfer oxygen around the body
D. the organs in it help absorb nutrients for the body

Answers

A-When the immune system is functioning properly, it can fight off disease-causing organisms including bacteria, viruses, and parasites as well as cancer cells while safeguarding healthy tissue.

Answer: A

Explanation:

Exercise results in skeletal muscles compressing veins which encourages blood to return to the heart. In this scenario, which of the following is correct

Answers

The exercise results in skeletal muscles compressing veins which encourages blood to return to the heart. In this scenario is that the preload increases. Option D

What are preloads?

Preload are also  known as the left ventricular end-diastolic pressure (LVEDP)

It is also known as the amount of ventricular stretch at the end of diastole. It is the heart loading up for the next big squeeze of the ventricles during the systole.

Preload is also affected by the rate of venous return and the venous blood pressure , which are affected by the volume of circulating blood and venous tone .

Preload is increased by exercising , or increase in blood pressure and neuroendocrine excitement .

Thus, the exercise results in skeletal muscles compressing veins which encourages blood to return to the heart. In this scenario is that the preload increases. Option D

The complete question is

Exercise results in skeletal muscles compressing veins which encourages blood to return to the heart. In this scenario, which of the following is correct?

a. End diastolic volume (EDV) decreases.

b. Stroke volume decreases

c. Venous return decreases.

d. Preload increases.

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__________ is the name of the specialized enzyme that unzips the bonds between bases.

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Helicase is the name of the specialized enzyme that unzips the bonds between bases.

By severing the hydrogen bonds between base pairs and unwinding the DNA molecule's two strands, an enzyme known as Helicase aids in unzipping the molecule. It binds to and dissolves the hydrogen bonds connecting the bases on the DNA strands. These hydrogen bonds continue to be broken as the helicase travels along the DNA molecule, separating the two polynucleotide chains.

Primase, a different enzyme, momentarily binds to each strand when the helicase separates them in order to build a foundation upon which replication can start.

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The structural commonality of the biceps brachii and the rectus abdominis is that they are __________.

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The structural commonality of the biceps brachii and the rectus abdominis is that they are parallel muscles whose fibers run along through the long axis of the body.

Together with the pyramidalis muscle, the rectus abdominis is a member of the anterior abdominal muscles. However, when considering the functional architecture, these two muscles, together with the three lateral abdominal muscles—the external oblique, internal oblique, and transversus abdominis make up the anterolateral abdominal wall.

The biceps brachii muscle, also known as the "biceps," is a big, thick arm muscle with two heads. The supraglenoid tubercle, located above the glenoid cavity of the scapula, is the location of the long head. It is extra synovial while being in the intracapsular area.

At the humeral head, the long biceps tendon curves sharply before continuing on in the bicipital groove (intertubercular sulcus). Ligaments in the capsular region (also known as the biceps pulley) hold this pivotal point in place.

Short head: Arises at the coracoid process of the scapula, where it partially fuses with the coracobrachialis origin tendon.

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the _______ is the central organ in the circulatory system. it has four chambers.

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Answer is the heart

There are four chambers of the heart, the left atrium and right atrium (upper chambers), and the left ventricle and right ventricle (lower chambers).

The complete sentence is "The heart is the central organ in the circulatory system. It has four chambers".

The heart, which pumps blood throughout the body, is an essential part of the circulatory system. It is commonly referred to as the "central pump" or the "central organ" of the circulatory system due to its crucial role in maintaining blood circulation.

The heart, a muscular organ, is located in the chest cavity and leans slightly to the left. Two top chambers, known as atria, and two bottom chambers, known as ventricles, make up the heart.

The two atria of the heart act as its chambers of reception. Both the left and right atriums of the heart receive blood that is returned to the heart by the body. The heart's two ventricles are its pumping chambers. They contract ferociously to force blood out.

Thus, the complete sentence is "The heart is the central organ in the circulatory system. It has four chambers".

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Some proteins in the ______ use the energy of the electrons donated by nadh and fadh2 to pump protons (h ) into the intermembrane compartment of the mitochondrion.

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Some proteins in the electron transport chain use the energy of the electrons donated by nadh and fadh2 to pump protons (h ) into the intermembrane compartment of the mitochondrion.

Electron transport chain means a series of four protein complexes that couple redox reactions which usually creates an electrochemical gradient that leads to the creation of ATP in a complete system named oxidative phosphorylation

What are proteins?

Proteins simply refers to those nitrogenous organic compounds or substances which have large molecules composed of one or more long chains of amino acids

So therefore, some proteins in the electron transport chain use the energy of the electrons donated by nadh and fadh2 to pump protons (h ) into the intermembrane compartment of the mitochondrion

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The inner surface of the eyelids and the exposed surface of the eye itself are covered by:_______

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The inner surface of the eyelids and the exposed surface of the eye itself are covered by Conjunctiva. The conjunctiva is a transparent mucous membrane that lines the inner surface of the eyelids and the anterior surface of the sclera. The sclera, or white of the eye, is strong tissue that wraps around the eyeball. It helps maintain your eyeball's shape and protects it from injury.

The ciliary body is the tissue which covers the inner part of the anterior segment of the eye coating. It is composed of the ciliary muscle and processes. On the surface of the ciliary body is the black ciliary epithelium, which produces the aqueous humors.

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Worms are classified into three phyla: flatworms, roundworms, and annelids. what is the feature that distinguishes them?

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Annelids are the feature that distinguishes them.

Annelids are segmented worms while roundworms are not segmented. further, annelids have a true coelom at the same time as roundworms have a pseudocolor. this means annelids have a well-developed mesoderm whilst roundworms lack a mesoderm. those are the primary capabilities that distinguish annelids from roundworms.

The 3 main malicious programs phyla are flatworms, roundworms, and segmented worms. Roundworms have a digestive tube that has openings. Segmented worms have a closed circulatory system wherein blood is enclosed in blood vessels. These are crucial steps in the evolutionary development of animals.

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An organism is viewed under low power and occupies approximately ¼ of the field diameter. The field diameter of this particular microscope under low power is 2000 µm. Calculate the actual size of the organism.

Answers

The actual size of the organism will be 5 µm.

Microscope magnification

The organism occupies 1/4 of the field diameter.

The field diameter is 2000 µm. So, the organism occupies 1/4 x 2000 = 500 µm.

The total magnification under the low power objective is 100.

100x = 500, x = 500/100 = 5 µm

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Explain why the simplest drawing to model the principle of independent assortment requires four chromosomes, but the simplest drawing to model the principle of segregation requires only two chromosomes

Answers

Independent assortment involves the independent segregation of the alleles of two separate genes on separate chromosomes.

What is the Principle of independent assortment How is it related to the Principle of segregation?

Understanding the law of segregation is necessary before defining independent assortment. According to the law of segregation, individual gamete cells receive two distinct, independently sorted genes during meiosis. On the other side, the random separation of the maternal and paternal DNA allows for a greater variety of genes.

Independent assortment refers to the independent segregation of two different genes' alleles on two different chromosomes. As a result, to create the simplest model of independent assortment, at least 4 chromosomes must be represented as two homologous pairs. On the other hand, only the two chromosomes of one homologous pair need to be drawn because segregation takes into account the separation of the two copies of a single gene carried on a single type of chromosome.

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How do xenoturbellida relate to hemichordates and nonvertebrate chordates?

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The deuterostome group's gene arrangement in the mitochondrial genome of Xenoturbella bocki is remarkably close to that of the chordates and hemichordates and hence to the ancestor deuterostome gene order. Inversion and breakpoint studies particularly point to a similarity to the hemichordates.

Previous morphological investigations imply that Xenoturbella could be classified as a bivalve mollusc, a basal bilaterian, a turbellarian flatworm, a sister group to the echinoderms(nonvertebrate), or a hemichordate. Recent genetic analyses that focus mostly on nuclear genes appear to favour the Ambulacraria (echinoderms + hemichordates). In contrast, a basal deuterostome location is supported by mitochondrial sequences.

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Which of the below features was not used to identify the early Homo distinction from Australopithecines

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The correct answer is reduction in tooth size.

The anatomy of the foot and pelvis are the feature of Australopithecus afarensis that is used to definitively classify this species as a hominin.

Australopithecines

Australopithecines are an adaptive radiation of early hominins, all of which were to some extent bipedal, had brains that were only slightly larger than those of apes, and had adaptations to a diet that included at least occasionally hard-to-chew items. They have been identified through research on perhaps ten species that lived in central, eastern, and southern Africa between 4.2 million and 1.0 million years ago. Understanding australopithecines is essential to comprehending not only the diversity of early hominins but also the origins of Homo. Our genus Homo probably originated from this radiation, albeit we are unsure from which species.

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What is the combination of an electrical gradient and a concentration gradient called?

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The combination of an electrical gradient and a concentration gradient called electrochemical gradient. It is a gradient of electrochemical potential for an ion that move across a membrane.

The  gradient has two parts -chemical gradient and electrical gradient. When unequal concentration of an ion across a permeable membrane  and the ions moves toward the membrane from the higher concentration to lower concentration through the process of simple diffusion.

If unequal distribution of charges towards the membrane then electric potential generates a force that drive ion diffusion until the equilibrium is attained and both sides are balanced. This electrochemical gradient is important to the operation of batteries and photosynthesis and cellular respiration or other biological processes.

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Digestion refers to the A. progressive dehydration of indigestible residue. B. mechanical breakdown of food. C. release of water, acids, enzymes and buffers by organs. D. mechanical and chemical breakdown of food.

Answers

Answer:

d is the correct answer

Explanation:

Answer:

[tex]\huge\boxed{\sf Option \ D}[/tex]

Explanation:

Digestion is:mechanical breakdown. (movement of muscles for catabolism)chemical breakdown. (Use of enzymes so that the food can be made smaller)

So, Digestion is both mechanical and chemical breakdown of food.

[tex]\rule[225]{225}{2}[/tex]

You are microscopic and stranded inside the right atrium during passive ventricular filling. How many potential escape routes do you have (assume you can swim with and against the current):

Answers

4 potential escape routes you have inside the right atrium during passive ventricular filling.

What Is the Pathway of Blood Through the Heart?

There are four chambers in the human heart.

Mammals and birds have double circulation.

The pathway of blood through the heart is as follows:

The deoxygenated blood from the body is received in the right atrium from the vena cava and enters the right ventricle through a tricuspid valve. It enters the pulmonary artery through the semilunar valve and goes to pulmonary circulation.The oxygenated blood from the lungs is received in the left atrium from the pulmonary veins and enters the left ventricle through the bicuspid valve. It enters the aorta through a semilunar valve and goes to the systemic circulation.

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please i need help right now!

Answers

The answer is oxygen :)
the correct answer would be B. Oxygen

Which instrument is designed to remove supragingival calculus from anterior teeth?

Answers

Periodontal curettes instrument is designed to remove supragingival calculus from anterior teeth.

What is periodontal curette ?

Periodontal curettes have a rounded back and toe, one face, and one or two cutting edges. They are often the tool of choice for removing supragingival calculi. When doing nonsurgical periodontal therapy as part of a patient's oral hygiene care, universal and Gracey curettes are frequently employed.

What type of instrument is periodontal curette ?

ultrasound instruments The main kind of treatment for eliminating plaque and calculus is ultrasound technology. These power-driven devices oscillate at incredibly fast rates, creating tiny vibrations that help eliminate calculus and subgingival plaque.

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which part of eubacteria is responsible for helping the cell to move

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There are some structures that can be responsible for moviment on bacterias, the most common are flagella, cilium and also pili.

What is an Eubacteria?

Eubacteria are part of one of the groups of Kingdom Monera (the other group is Archaea). These bacteria are prokaryotic organisms and represent the largest number of species within the Monera Kingdom. All bacteria (excluding archaebacteria) and cyanobacteria are part of the Eubacteria Group.

They are unicellular organisms that do not have a defined nucleus or membranous organelles. They can be classified according to their shape, with the most common shapes being spherical, rod and spiral.

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Creta, M. et al. Inhibition of Androgen Signalling Improves the Outcomes of Therapies for Bladder Cancer: Results from a Systematic Review of Preclinical and Clinical Evidence and Meta-Analysis of Clinical Studies. Diagnostics (Basel) 11, doi:10.3390/diagnostics11020351 (2021).

Answers

Bladder cancer (BCa) is an endocrine-related tumor and the activation of androgen signaling pathways may promote bladder tumorigenesis.

Abstract:

We compiled the research on the effects of altering androgen signaling pathways on Bladder cancer therapy outcomes, both preclinical and clinical. A thorough analysis was done in December 2020. The following criteria were met by the papers we included: examining the effects of androgen signaling regulation on the results of Bladder cancer treatments through innovative preclinical and clinical studies. Eight clinical trials and six preclinical studies were found. Preclinical research shows that the activity of radiation, doxorubicin, cisplatin, gemcitabine, and the Bacillus Calmette Guerin may be affected by the modification of androgen receptor-related pathways.

Patients receiving treatment with 5-ARIs or androgen deprivation therapy (ADT) have a considerably lower relative risk of developing Bladder cancer again after transurethral resection of the bladder tumor (TURBT) (Relative risk: 0.50, 95 percent confidence interval: 0.30-0.82; p = 0.006). In patients using 5-ARIs, subgroup analysis showed a relative risk of BCa recurrence of 0.46 (95% CI: 0.22-0.95; p = 0.040). The ratio of T1 Bladder cancer patients in treated/control groups and the relative risk of BCa recurrence were found to be significantly negatively correlated. A promising technique to lower the recurrence rate of Bladder cancer, particularly in patients with early-stage disease, may involve 5-ARI therapy. To corroborate these early findings, additional research is required.

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Biosecurity includes actions and planning to prevent unauthorized?

Answers

Biosecurity includes actions and planning to prevent unauthorized entry into laboratories, removal or misuse of microbiological agents or hazardous agents

What is Biosecurity?

Biosecurity refers to the protection and accountability of highly sensitive and mostly lethal biological agents and toxins in order to prevent them from exposure to the general public.

Biological toxins must be properly labelled and sealed so as to avoid inadvertent exposure. These toxins pose immense and immediate health risks to individuals exposed to them.

Action and planning is therefore required to ensure these biological agents are properly secured and to prevent unauthorized access, removal or exposure to biological hazardous agents.

Maximum security laboratories are often used to test and experiment with these biohazards.

In conclusion, biosecurity ensures that individuals without authorized access do not come in contact wuty biohazards.

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Heidi and gunther live with their biological parents. what type of family would this be called?

Answers

This type of family is called a nuclear family.

What is a family?

A family is defined as the smallest unit of the society that is made up of individuals whom are related by blood, marriage or adoption.

There are different types of family which include the following:

Nuclear Families: This is made up of a father, a mother and their children.

Single-Parent Families;

Step Families.

Grandparent Families:

Childless Families:

Extended Families:

The importance of family include the following:

Family is the primary source of learning for children.

Family provides the individual with moral support when necessary.

A family gives us stability.

A family makes us responsible for our duty.

Family gives us some level of security in the society.

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For a fee, a copy of an inspection report filed by a licensed structural pest control operator may be obtained from the Structural Pest Control Board by:

Answers

For a fee, a copy of an inspection report filed by a licensed structural pest control operator may be obtained from the Structural Pest Control Board by anyone who requests it and pays the fee.

What is an inspection report?An inspection report is a document generated by a company that undertakes inspections for a client in order to explain a structure or object. Inspection reports are critical instruments for ensuring the quality of buildings, facilities, and goods.

Structural Pest Control Board:

Structural pest control is the control of household pests (including but not limited to rodents, vermin, and insects) and wood-destroying pests and organisms, as well as other pests that may infiltrate households or structures, such as railroad cars, ships, docks, trucks, airplanes, or their contents.Anyone who wants and pays the charge may acquire a copy of an inspection report issued by a licensed structural pest control operator from the Structural Pest Control Board.

Therefore, for a fee, a copy of an inspection report filed by a licensed structural pest control operator may be obtained from the Structural Pest Control Board by anyone who requests it and pays the fee.

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________is when the kidneys produce too much hydrogen or retain too much hydrogen which leads to an increase in carbonic acid production. Or the kidneys excrete too much base

Answers

Metabolic acidosis is when the kidneys produce too much hydrogen or retain too much hydrogen which leads to an increase in carbonic acid production. Or the kidneys excrete too much base.

What is metabolic acidosis?The primary function of the kidney is to purge toxins from the blood and convert waste products into urine. Around 160 grams of weight and one to one and a half litres of urine are excreted daily by each kidney.When the kidneys do not properly eliminate acids from the blood into the urine, renal tubular acidosis (RTA) ensues. The result is an excessively high blood acid level, or acidosis. While having some blood acid is natural, having too much acid can cause numerous biological functions to become unbalanced.When there is too much acid in the body's fluids, it is called acidosis.When your kidneys and lungs are unable to maintain a healthy pH level in your body, acidosis results. Acid is created by numerous bodily activities.

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Destruction of the supra-optic nucleus of the hypothalamus would have which result?
A) loss of ADH secretionB) loss of GH secretionC) loss of melatonin secretionD) loss of emotional responseE) loss of loss of regulatory factor secretion

Answers

A )loss of ADH secretion.

Destruction of the supra-optic nucleus of the hypothalamus can cause loss of ADH secretion. Reason behind this is antidiuretic hormone are synthesized in hypothalamic supra optic nerves .Hence, destruction of supra optic nerve in the hypothalamus will alter the production and secretion of ADH . ADH is stored in posterior pituitary gland and is released into the circulation in response to extracellular fluid changes by the osmoreceptors in the brain.

Deficiency of ADH can cause or insensitivity of the kidney to ADH also known as nephrogenic diabetes insipidus. These patients, if untreated, have the predictable result of dehydration, hyperosmolality, hypovolemia, and eventual death in severe cases.

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Use what you know about fossils to choose the correct answers from the drop-down menus to complete each
statement.
If the same organism appears on two different continents, this is evidence of
Fossils show how organisms change, or
v over time.
Fossils provide a record of animals that are
x], or no longer living.

Answers

The answer to the questions include the following:

If the same organism appears on two different continents, this is evidence of plate movement.Fossils show how organisms change, or evolve over time.Fossils provide a record of animals that are extinct or no longer living.

What is a Fossil?

This is defined as the remains of plants and animals which have been preserved in the earth crust. Fossils helps in the study of organisms and how they evolved from others as a result of a change in their living conditions. This happened so as to enable them survive thereby giving rise to more fit offspring.

Fossils provide a record of animals that are extinct or no longer living such as dinosaurs etc. Similar organisms being present on different continents is as a result of plate movement thereby making it the most appropriate choices.

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