The correct sequence for western blotting is:

1. Proteins are denatured by sodium dodecyl sulfate (SDS), and by boiling.
2.Transfer proteins to the nitrocellulose membrane.
3. Performing the polyacrylamide gel electrophoresis (PAGE).
4. Incubate the nitrocellulose membrane to primary antibodies and secondary antibodies subsequently.

a. 1,2,3,4
b. 4,1,3,2
c. 1,3,2,4
d. 1,3,2,4

Answers

Answer 1

Answer:

3.page is this that answer

Explanation:

a.1,2,3,4

Answer 2

The correct sequence for western blotting is: C. 1,3,2,4

Blotting can be defined as an analytical technique that is designed for the analysis of biological macromolecules such as:

Lipids.Nucleic acid.Proteins.Carbohydrates.

For western blotting, the correct sequence of analyzing biological macromolecules such as proteins is:

1. The denaturation of protein through the use of sodium dodecyl sulfate (SDS) and by boiling.

2. The performance of polyacrylamide gel electrophoresis (PAGE).

2.The transfer of proteins to the nitrocellulose membrane.

4. Lastly, the incubation of the nitrocellulose membrane to subsequently form primary antibodies and secondary antibodies.

Read more: https://brainly.com/question/14681125


Related Questions

Complete each sentence by putting the labels to the appropriate blanks. Then place each sentence in a logical order beginning with blood entering the right side of the heart.
a. Blood enters the right ventricle from the right atrium through the right atrioventricular valve which is also known as the_________.
b. From the left ventricle, the blood is pumped up to the___________ through the left semilunar valve which is sometimes referred to as the___________.
c. Beginning with the return from the systemic circulation, blood enters the________.
d. The right ventricle contracts next, which forces blood up through the right semilunar valve which is otherwise known as the__________.
e. After circulating through the blood returns to the_______.
f. From the left atrium, blood moves through the left________ which is also referred to as the________ or mitral valve.
1. bicuspid
2. tricuspid valve
3. right atrioventricular valve
4. right atrium
5. aortic valve
6. left atrioventricular valve
7. atrioventricular valve
8. pulmonary valve
9. left atrium
10. aorta
11. pulmonary capillaries
12. systemic capillaries

Answers

Answer:

c. Beginning with the return from the systemic circulation, blood enters the right atrium.

a. Blood enters the right ventricle from the right atrium through the right atrioventricular valve which is also known as the tricuspid valve

d. The right ventricle contracts next, which forces blood up through the right semilunar valve which is otherwise known as the pulmonary valve .

e. After circulating through the pulmonary capillaries, the blood returns to the left atrium.

f. From the left atrium, blood moves through the left atrioventricular valve which is also referred to as the bicuspid or mitral valve.

b. From the left ventricle, the blood is pumped up to aorta the through the left semilunar valve which is sometimes referred to as the aortic valve.

Explanation:

c. Beginning with the return from the systemic circulation, blood enters the right atrium.

a. Blood enters the right ventricle from the right atrium through the right atrioventricular valve which is also known as the tricuspid valve

d. The right ventricle contracts next, which forces blood up through the right semilunar valve which is otherwise known as the pulmonary valve .

e. After circulating through the pulmonary capillaries, the blood returns to the left atrium.

f. From the left atrium, blood moves through the left atrioventricular valve which is also referred to as the bicuspid or mitral valve.

b. From the left ventricle, the blood is pumped up to aorta the through the left semilunar valve which is sometimes referred to as the aortic valve.

S100A8 sex chromosome? yes or no

Answers

Answer:

yes !!

Explanation:

because it's yes !!

Answer:

YES

Explanation:

I know this because i looked it up

The role of the ___________ branch of the autonomic nervous system mediates control of organ processes when the body is essentially ______.

Answers

Answer: parasympathetic; at rest

Explanation: The parasympathetic nervous system branch of the autonomic nervous system is largely responsible for the relaxation of the body especially at rest where it undoes the activities of the sympathetic branch by decreasing respiration, heart rate and then increasing the body's digestion rate. As such, this branch is also responsible for digestion response in a relaxed, resting, or feeding states. The role therefore of the parasympathetic branch of the autonomic nervous system mediates control of organ processes when the body is essentially at rest.

Hereditary fructose intolerance is a genetic condition caused by a variant in the ALDOB gene, which is critical for fructose metabolism. Infants with this condition do not initially display symptoms, but as they are transitioned from breast milk or formula to solid foods, in particular fruit-based foods, they begin to display symptoms, which include vomiting, diarrhea, and low blood sugar. Individuals who do not consume fructose do not display these symptoms. Which of the following describes this condition?

a. It is incompletely penetrant, due to environmental factors.
b. It has variable expressivity, due to environmental factors.incorrect
c. It is incompletely penetrant, due to genetic factors.
d. It has variable expressivity, due to genetic factors.

Answers

It has variable expressivity due to the environmental factors.

The variation in expressivity occurs due to the environmental factors of that individual. Expressivity describes the differences observed in the phenotype between two individuals that belongs to same genotype.

In the condition, when the patient is allowed to consume the fructose based food, so it expresses the change in its body due to no tolerance against fructose so we can conclude that the change occurs due to environment not due to genetics.

https://brainly.com/question/24350695.

explain about Biogenetic theory?

Answers

Answer:

biogenetic theory mean the development and evolution processed by Ernst heackel in Germany in the 1860 s . it is one of the several recapitulation which posit that the stage of development for an animal embryo as the same as other animal or adults stage or forms.

Identify the effects of the vagus nerve on the following components of the digestive system Salivary glands Pyloric sphincter (gastric emptying) Smooth muscle in the gallbladder Smooth muscle in the duodenal papilla (sphincter of Oddi)

Answers

Answer:

Vagus nerve is the 10th cranial nerve. It is long start from brain and distributed over head, thorax and abdomen.

Explanation:

1.   In the salivary gland the secretory and vasodilator effect on afferent nerve is mediated mainly by parasympathetic reflex which is given by vagus nerve.

2.   Pyloric sphincter is the part present between the stomach and small intestine which regulate the flow of food particles from stomach to the intestine. Vagus nerve control the activities of pyloric sphincter. Vagus nerve help the pyloric sphincter to relax.

3.  Efferent nerves of vagus nerve help the gall bladder to contract. Vagus nerve is stimulating the gastric tract to contract. So the vagus nerve stimulation promotpromotprthe production of CCK hormone and it will lead to the contraction and secretion of hormones from the gallbladder. Those contractions will help the gallbladder to empty the hormones from gallbladder.

4.  The efferent nerves of vagus nerves act on the smooth muscles of duodenal papila. As above says it lead to the contraction of smooth muscles and also promote the production of CCK.


Please select the correct chorological order for the following events:
A. Treaty of New Echota, Black Hawk War Ends, Black Hawk War Ends
B. Black Hawk War Ends, Treaty of New Echota, Treaty of Fort Laramie
C. Treaty of Fort Laramie, Black Hawk War Ends, Treaty of New Echota
D. Treaty of New Echota, Treaty of Fort Laramie, Black Hawk War Ends
Reset Selection

Answers

Answer:

C treaty of fort laramie, black hawk war end, treaty of new echota

identify the components ( parts) of DNA

Answers

Answer:

Phosphorus group, a sugar(like deoxyribose or ribose), and a nitrogenous base

what is meant by oesophagus

Answers

Answer:

The oesophagus (gullet) is part of the digestive system, which is sometimes called the gastro-intestinal tract (GI tract). The oesophagus is a muscular tube. It connects your mouth to your stomach.

Answer:

the part of the alimentary canal which connects the throat to the stomach. In humans and other vertebrates it is a muscular tube lined with mucous membrane

Put the vocabulary words in order from largest level of organization to smallest. Reorder answers 1. Population Reorder answers 2. Organism Reorder answers 3. Organ Reorder answers 4. Atom Reorder answers 5. Biosphere Reorder answers 6. Species Reorder answers 7. Organ system Reorder answers 8. Community Reorder answers 9. Cell Reorder answers 10. Ecosystem Reorder answers 11. Organelle Reorder answers 12. Tissue Reorder answers 13. Molecule

Answers

Answer:

The level of organization in order from largest to smallest is;

1. Biosphere

2. Ecosystem

3. Community

4. Population

5. Species

6. Organism

7. Organ system

8. Organ

9. Tissue

10. Cell

11. Organelle

12. Molecule

13. Atom

Explanation:

All living things on Earth are arranged in an hierarchical level of organization. The order in descending way is as follows:

1. Biosphere- Biosphere refers to the part of the Earth that constitutes all living organisms in interaction with their environment. It is the total of all ecosystems on Earth.

2. Ecosystem: Ecosystem refers to a group of living organisms i.e plant, animal and microbes interacting with each other and their abiotic environment e.g water, air etc. An ecosystem comprises of several communities.

3. Community- Community refers to a group of organisms interacting with each other at a particular time and habitat. A community is made up of two or more populations.

4. Population- Population refers to a group of organisms of the same species living together in the same habitat and capable of interbreeding.

5. Species- A species is a group of organisms usually with the same appearance and capable of producing fertile offsprings by interbreeding.

6. Organism- An organism is an individual living thing i.e. plant, animal, microbe. An organism is made up of several organ systems that work together to make it whole.

7. Organ system- Organ system refers to a group of organs working in an interconnected manner to perform certain functions in an organism.

8. Organ- An organ is a structure in an organism that performs a specific function.

9. Tissue- A tissue is a group of cells working together for the same purpose.

10. Cell- A cell is the basic and fundamental unit of life, which is the building block of all living organisms.

11. Organelles- An organelle is a specialized structure in a cell that performs specific functions. e.g. nucleus, mitochondria etc.

12. Molecule- A molecule is the smallest unit of a chemical compound responsible for the chemical identity of that compound. A molecule is made up of two or more atoms chemically bonded together.

13. Atom- An atom is smallest indivisible unit of mattter that partakes in chemical reactions. Atom is considered the smallest unit of matter.

Answer:

:)

Explanation:

If you kill someone while driving under the influence, you can

Answers

Answer:

you can go to jail. in most cases you will go to jail

Explanation:

Which of the following is the term for tightly packed sheets of cells that cover organs and outer surfaces?
a. Epithelial tissue
b. Connective tissue
c. Muscle tissue
d. Nervous tissue

Answers

Answer:

Epithelial tissue

Explanation:

The correct option would be the epithelial tissue.

Epithelial tissues are made up of tightly arranged cells and primarily functions as protective covers for organs and outer surfaces. Usually, a side of the component cells in epithelial tissue are in contact with organs which they are covering through a non-cellular basement membrane and the other side of the cells are free.

The non-cellular basement membrane that connects the cells in epithelial tissues to organs and surfaces is secreted jointly by the epithelial and connective tissues and usually made up of protein and carbohydrate.

Classify the descriptions as pertaining to nucleosides, nucleotides, or both nucleosides and nucleotides.

a. Do not contain a phosphate group
b. The product when a base bonds to carbon 1 of ribose or deoxyribose
c. Contain a base and a monosaccharide monosaccharide, and a phosphate group

Answers

A. Do not contain a phosphate group, and are the product when a base bonds at C1 of ribose or deoxyribose

B. Contain a base, a monosaccharide, and a phosphate group, are the monomers of nucleic acids, and can be named deoxyguanosine 5'- monophosphate


C. Are found in RNA and DNA, contain a base and a monosaccharide, and may contain either ribose or deoxyribose

The correct description is as follows:

Do not contain a phosphate group - nucleosidesThe product when a base bonds to carbon 1 of ribose or deoxyribose - nucleosideContain a base and a monosaccharide and a phosphate group - nucleotides

What is a nucleoside and nucleotide?

Nucleoside is a a compound consisting of a nitrogenous base (purine or pyrimidine) linked to a sugar e.g. adenosine.

Nucleotide is a compound consisting of a nucleoside linked to a phosphate group i.e. nucleoside + phosphate group = nucleotide

Therefore,

A substance that do not contain a phosphate group - nucleosidesThe product when a base bonds to carbon 1 of ribose or deoxyribose - nucleosideA substance that contain a base and a monosaccharide and a phosphate group - nucleotides

Learn more about nucleotides at: https://brainly.com/question/13185536

Consider a material for a vascular graft application with proteins adsorbed to its surface and a second material with proteins covalently attached to its surface. For which material would you expect the protein layer to be more stable under flow conditions similar to those found in a blood vessel? Why?

Answers

Answer is given below :

Explanation:

Individual-specific proteins are placed on the graft material so that the body does not recognize the graft as a foreign substance and treats it as a part of the body’s natural tissue structure. If the body recognizes the graft as a foreign substance, it produces an immune response against it and leads to a graft rejection reaction by the body. In order for these proteins to attach to grafts, a covalent bond for absorption must be preferred. Absorption is a reversible process and, if used, can lead to de-absorption of bound proteins, including blood, after dissolving by turbulence and pulling to the grafting material by blood flow (velocity up to 40 cm/sec). The remaining infectious tissue is detected foreignly, activating the immune response.  On the other hand, covalent bonding is irreversible in nature and vascular flow does not have a disruptive effect on the bond between the graft and the protein, reducing the stable state of the graft and the possibility of rejection.

Does climate change tend to be more abrupt or gradual? Explain your response.

Answers

Answer:

Gradual**

Explanation:

This seems to be an opinion based question but i mean if you look back at history in the early ages or pre- industrialization the worls had very little pollution but gradually as factories cars and other things started being invented we as humans started releasing harmful substances into our atmosphere which began slowly craving away at the ozone layer and til date we are doing the same thing and are facing climate change/ global warming. The world did not suddenly face this problem.

I hope this makes sense

Keeping in mind that a crayfish is saltier than the fresh water it occupies, and remembering the idea of osmosis, note that the antennal gland deals directly with the consequences of this issue. What do you suppose the function of the antennal gland is

Answers

In the antennal gland of the freshwater crayfish (Astacus), fluid is filtered from the blood into an end sac and passes through a tubular labyrinth, where ions are reabsorbed, to produce a hypotonic urine that passes via a renal tubule to the bladder.

A purebred tall pea plant is cross-pollinated with a tall, heterozygous pea plant. Use a Punnett square to determine the probability the offspring inherita
recessive short allele. (I point)
75%
25%
0%
50%

Answers

Answer:

The correct answer is - 25%

Explanation:

A cross between true tall pea plant and heterozygous tall pea plant is the cross of tall allele whic is TT and heterozygous which is Tt, so the gametes will be formed would be - T and T by true tall plant and T, and t allele by heterozygous plant.

The Punnett square of this cross is attached with the answer, where 2 heterozygous offspring and two tall offspring produced. In which there is only two recessive short allele formed in this generation out of 8 alleles.

So the probability of short allele would be:

= (2/8) *100

= (1/4) *100

= 25%

which of the following is not a type of light microscope?

A. transmission electron microscope
B. phase contrast microscope
C. bright field microscope
D. stereo microscope ​

Answers

Answer:

A. transmission electron microscope.

Describe Dr. Vellend's unifying theory of ecological communities (the factors that determine community structure). Where do concepts like competition, predation, habitat suitability, disease and parasitism, and disturbance (most of what we covered this semester) fit into his theory

Answers

Answer and Explanation:

Community ecology is a discipline that studies the organism's communities and the manner in which populations of species interact with each other within a particular habitat. Mark Vellend is an eco-evolutionist that developed a theoretical framework of ecological communities from a unified functional perspective. This theory is based on four forces of evolutionary biology: selection, drift, dispersal and speciation. This theory is used to explain how species interactions including parasitism, competition and predation may influence population fluctuations. On the other hand, this theoretical framework can also be used to understand how colonization, disturbance and habitat suitability components alter the dynamics of interacting species.

population of squirrels live together in forest an event occurred that caused the population to diverge into two different species over a long period of time which of the following is most likely the event occurred

Answers

A natural disaster.

Explanation:

A natural disaster would most likely have occurred to have the population of squirrels completely diverge from each other in order for them to become two separate species.

If a tornado, flood, earthquake, or another type of a natural disaster were to happen, it may have caused drastic damage to the ecosystem and environment of the squirrels. This would most likely cause the squirrels to split up, or diverge from each other and go to separate places in search of a new home.

Over time, these squirrels will reproduce with their own group. Each new group of squirrels, as they are both in different environments now, will have different adaptations from each other. This will eventually make it so they will never be able to reproduce with each other again if they were to meet, as they are now completely different species of squirrel.

Answer:

ITS B   A natural disaster occurred that separated squirrels.

Explanation:

which compound is produced during regeneration​

Answers

Answer:

RuBP

Explanation:

Ribulose 1,5-bisphosphate (RuBP) is an organic substance that is involved in photosynthesis. It is a colourless anion, a double phosphate ester of the ketopentose (ketone-containing sugar with five carbon atoms) called ribulose. Salts of RuBP can be isolated, but its crucial biological function happens in solution.

Match each part of Sammi's model to the bodily structure it represents. One bodily structure has already been labeled for you.

Answers

Answer:

corrugated cardboard tube - digestive tract

irregular surfaces on the corrugated tube - villi

pump - heart

Plastic tubing - blood vessels

Explanation:

The corrugated cardboard tube represents the digestive tract.  The give away was the presence of the irregular surfaces within the tubes. This is called the villi. The villi is responsible for the absorption of food after digestion into the body.

Irregular surfaces on the corrugated tube represent the villi. They are tiny hair-like structures that are in the small intestine, that are responsible for the absorption of food into the bloodstream.

The pump represents the heart. The heart is the only organ in the body that provides the pressure force that pushes the blood throughout the body. In this model, the pump will most likely be performing the same function.

The plastic tubings are meant to contain one form of fluid or the other. Judging from that, they can be said to represent the blood vessels. This is because they are connected to the heart and are meant to transport bodily fluids around the body

Answer:

corrugated cardboard tube - digestive tractirregular surfaces on the corrugated tube - villipump - heartPlastic tubing - blood vessels

Explanation:

Which of the following is an example of reproductive isolation?

Answers

Answer:

the example of reproductive isolation is temporal isolation, ecological isolation, behavioral isolation, mechanical isolation.

The force of a muscle contraction is higher when the muscle is stimulated every 0.5 secs than when stimulated with the same voltage every 0.05 seconds.

a. True
b. False

Answers

Answer:

b. False

Explanation:

Increasing the frequency of muscle stimulation causes wave summation to occur, which causes an overlap of muscle twitch. When this muscle summation happens, the force generated in the muscle increases. This means that stimulating the muscle every 0.5 seconds will generate less muscle contraction force than when stimulated every 0.05 seconds  

Drag the correct tiles to the box. Not all tiles will be used.
Identify the goods or animals that Europeans introduced to the Americas.
turkeys
wheat
corn
horses it is not horses because it said it was wrong

Answers

Answer:

the eupopeans introduced horse to the americans, as the native american living there already had turkeys,corn,and wheat.

Which scientific questions can be disproved by conducting tests? Select all that apply. Do video games negatively impact mental health? Will my published scientific paper be successful? Can monkeys communicate with each other? Are dogs the most responsible type of pet? Are vegetables good for growing strong bones?

Answers

Answer:

Do video games negatively impact mental health?

Are vegetables good for growing strong bones?

Explanation:

A scientific question is one that can be subjected to experimental verification. Experimentation is then used to obtain valid data which can be used to answer the question.

A good scientific question must contain a dependent and an independent variable. The independent variable is manipulated and its effect on the dependent variable is observed.

In the two options chosen;

Effect of video games on mental health can be observed by conducting a research experiment on people who play video games and those who do not play video games.

Effect of vegetables on strong bones can be studied by conducting a research experiment using people who eat vegetables and those who do not eat vegetables.

A researcher wants to measure physical height in as much detail as possible. Which level of measurement does she employ?

Ratio level

Interval level

Ordinal level

Nominal level

Answers

ʏᴏᴜʀ ᴀɴsᴡᴇʀ

Ratio level is the correct option.

ᴇxᴘʟᴀɪɴᴀᴛɪᴏɴ:

Scales of measurement provide information regarding the measurements and the values that have been assigned to the variables within it. Ratio level of data is a quantitative type of data and they have an absolute zero.

function of carbohydrates​

Answers

The main function of carbohydrates is to provide your body with energy. Carbohydrates are one of three macronutrients in our diet the other two being fat and protein. Carbohydrates are digested and broken down into glucose which than is used to produce ATP in your bodies cells through cellular respiration. Then your cells use it to fuel your body so it can use that energy.

Answer:

Alongside fat and protein, carbohydrates are one of the three macronutrients in our diet with their main function being to provide energy to the body. They occur in many different forms, like sugars and dietary fiber, and in many different foods, such as whole grains, fruit and vegetables.

Explanation:

What are the following is considered a receptive a fine touch

Answers

Explanation:

This system is responsible for all the sensations we feel – cold, hot, smooth, rough, pressure, tickle, itch, pain, vibrations, and more. Within the somatosensory system,there are four main types of 1.receptorsme chanoreceptors, 2.thermoreceptors,3. pain receptors, and 4. proprioceptors.

Which of the following would be most likely to increase erosion rates?
A. A coastline with a well defined beach.
B. Waves breaking at the foot of a cliff
C. Waves with a very long fetch (have traveled far).
D. All of these

Answers

Answer:

D

Explanation:

we all need that all of then helps to increase erosion rates

D

This is because all of these have a major effect on the erosion rates and over time you would see a major difference with all the factors in play
Other Questions
A property sold for $198,000 with a listing commission of 8%. The selling office is to receive 40% of the total commission. How much will the listing salesperson receive if she is paid 60% of the amount retained by the listing broker? Rosa es simptica. Lgico Ilgico 2. La madre de Rosa necesita hacer diligencias. Lgico Ilgico 3. Rosa es una chica muy obediente. Lgico Ilgico 4. El padre de Rosa es ms estricto que la madre. Lgico Ilgico 5. Es probable que Rosa coma pizza. Lgico Ilgico The decrease in the water table due to overuse of water. The cost for an upcoming field trip is $30 per student. The cost of the field trip C. in dollars, is a function of the number of students x.Select all the possible outputs for the function defined by C(x)=30a. 20b. 30c. 50d. 90e. 100 Latin Help!!!! translate 1. The good farmer let the children play in his fields. 2. I see the wild animals (neuter) in the zoo. 3. They gave the small boy a new basketball. find the case, gender and number for the sentences. What is it called when an economic player avoids a choice because the opportunity cost is too high?(1 point)positive incentivetrade-offrational decisionnegative incentiveQuestion 2A) What occurs when individuals make decisions in which benefits exceed costs?(1 point)They are removing the opportunity costs of decisions.They are using a rational decision-making model.They are making irrational economic decisions.They are eliminating all trade-offs of the decision.Question 3A) What occurs when people make economic decisions in which the costs exceed the benefits?(1 point)They are losing the benefits of negative externalities.They are taking advantage of opportunity costs.They are using their time and resources efficiently.They are making irrational economic decisions.Question 4A) Which of the following is not one of the steps of the rational decision-making model?(1 point)performing an analysisdefining the problemidentifying alternativesdetermining productivityQuestion 5A) Kareem wants to make the most logical choice about what do after high school. Which advice would help him do that?(1 point)Make a choice based on costs exceeding benefits. Make a choice with no opportunity cost.Make a choice based on a rational decision-making model.Make a choice with no trade-offs. Each packet of the cooking oil weighs 2/5th of a kilogram and one kilogram of the cooking oil costs $6.5. Sara went to the grocery shop to buy some items to stock her kitchen. If she bought 8 packets of the cooking oil, how much money did she spend? A $19.60 B $18.20 C $20.80 D $23.40 One long wire carries a current of 30 A along the entire x axis. A second long wire carries a current of 40 A perpendicular to the xy plane and passes through the point (0, 4, 0) m. What is the magnitude of the resultant magnetic field at the point y Please help!! ASAP!! Read the paragraph, which has an error in adjective-noun agreement. Then, answer the question.La Sra. Estela Smith y el Sr. Alonso son maestros agradables. Los lunes y los mircoles tienen clases llenas de estudiantes ocupados. Los maestros son cuidadosas con sus estudiantes y se divierten diariamente.Select the option that corrects the error in noun-adjective agreement. clases-lleno estudiantes-ocupadas maestro-agradables maestros-cuidadosos What type of polynomial is: sqrt 7x^4 - x^3 Please helpppp anyone fast Which of the following are the correct steps to solve the equation below? 5x + 9 = 39A----Add 9 first and then divide by 5B----Add 9 first and then multiply by 5C-----Subtract 9 first and then divide by 5D----Subtract 9 first and then multiply by 5 Mack is constipated. Safe recommendations include all of the following EXCEPT: a. eat 30 grams of fiber per day b. drink more fluid c. use mineral oil routinely and liberally d. eat more whole grains put the verbs in the present simple tense in the following sentences. a.Rita .........( get up)at 6 o'clock everyday . b.I .........(walk) to school on foot. c.my sister always........(watch) cartoon. d.our school...........( start) at 3 o'clock. e.Hari ......... (play) football with his friends. f.My parents ........( work) hard. How could you narrow your topic about going to see a new movie? Both chlorine and fluorine are represented by a green modeling piece that has 4 holes. Is using the same piece for two different atoms acceptable? Why or why not What is the solution to:[tex] \frac{5}{8} = \frac{m}{12} [/tex]HELP! answer if you can. What does Carlos language reveal about yours his character Determine the number of degrees of freedom for the two-sample t test or CI in each of the following situations. (Round your answers down to the nearest whole number.)a. m = 12, n = 15, s1 = 4.0, s2 = 6.0b. m = 12, n = 21, s1 = 4.0, s2 = 6.0c. m = 12, n = 21, s1 = 3.0, s2 = 6.0d. m = 10, n = 24, s1 = 4.0, s2 = 6.0 I NEED HELP ANSWERING THESE QUESTIONS FIRST ANSWER GET BRAINLIEST!