Why should a scientist use a plasmid with a GFP gene when inserting a new gene?

A) The GFP gene will make cells glow green in ultraviolet lights, confirming that the gene
insertion worked.

B) The GFP gene will remove other genes, making it easier to see the effects of the new
gene.

C) The GFP gene is not well understood, so inserting it would be a valuable experiment all
on its own.

D) The GFP gene will stop the gene insertion from falling apart, making the results more
reliable.

Answers

Answer 1

Answer:

the answer is a

Explanation:

the GFP gene will make cells glow green in ultraviolet lights, confirming that the gene intersection worked.

Answer 2

A scientist use a plasmid with a GFP gene when inserting a new gene  because it makes the phenotypes that is easily detectable. The GFP gene will make cells glow green in ultraviolet lights, confirming that the gene insertion worked.

What is a GFP plasmid  ?

GFP plasmid is an engineered plasmid to be used in the biotechnology where the use of GFP will be in order to make up the genetic  modified organisms.

The GFP plasmid will make up the multiple number of reporter genes that will help to detect the mutations and the inserted gene . The most notice GFP gene that will be detected is GFP that is green fluorescent protein and the ampicillin resistant gene that will make the gene look different from other types of genes present.

The GFP gene will make cells glow green in ultraviolet lights, confirming that the gene insertion worked. This can be detected by the gel running when the screen is seen.

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Related Questions

What is a null hypothesis?

A. a hypothesis stating that there is no
relationship between the independent and
dependent variable
B. a hypothesis stating that there is a positive
correlation between data sets
C. a hypothesis stating that there is a negative
relationship between the data and the expected
values
D. a hypothesis that is rewritten from the
original after an experiment is ran

( acellus academy )

Answers

Answer:

A

Explanation:

a hypothesis stating that there is no relationship between the independent and dependent variable.

(ANSWER)

When you mix the primary colors of light, you produce the color:
a. yellow
b. green
c. white
d. black

Answers

Red and green light make yellow. And when all three primary colors of light are combined, we see white light.

Answer: White

Explanation: when all 3 of the primary colors are mixed you get a white light

1) You have an electropherogram with a clean, three-locus profile:
STR locus #1 is 10, 14
STR locus #2 is 30 (only)
STR locus #3 is 3, 6
What is the random match probability of this profile? Fill in the blank:
"Approximately one in every
people, drawn randomly from the human
population, would be expected to have this three-locus profile.

Answers

Following the product probability rule and assuming independence among loci, the random match probability of this three-locus profile is 0.000853. See below the procedure for question number 2.

What does the product probability rule state?

The product probability rule allows us to calcuate the probability of occurrence of two or more events at the same time. It is about a joint probability of two or more events that might happen simultaneously, not excluding each other.

This rule is based on the dependence or independence of the events. Two events, A and B, are independent of each other if one of them does not affect the occurrence of the other one.

The rule establishes that the occurrence probability of two independent events -A and B- together is the multiplication product of the probability of occurring A by the probability of occurring B.

P(A∩B) = P(A) x P(B)

In the exposed example,

STR locus #1 has three alleles → 10, 11, 14STR locus #2 has four alleles → 27, 28, 28.2, 30STR locus #3 has three alleles → 3, 6, 7

The frequency of each allele is detailed in the table.

According to the electropherogram, the profiles are

STR locus #1 → 10, 14 → Heter0zyg0usSTR locus #2 → 30 → H0m0zyg0usSTR locus #3 → 3, 6 → Heter0zyg0us

Knowing the allele frequencies, we can get the genotypic frequencies,

   Locus               Allele frequency            Genotypic frequency

STR locus #1

10                                 0.22                    2xpxq = 2 x 0.22 x 0.18 = 0.079

14                                 0.18            

----------------------------------------------------------------------------------------------------  

STR locus #2

30                                 0.22                    p² = 0.22² = 0.048

----------------------------------------------------------------------------------------------------  

STR locus #3

3                                   0.45                    2xpxq = 2 x 0.45 x 0.25 = 0.225

3                                   0.25            

----------------------------------------------------------------------------------------------------

So, the probability, P, of getting these genotypes are,

P ₍₁₀₋₁₄₎ = 0.079P₍₃₀₋₃₀₎ = 0.048P₍₃₋₆₎ = 0.225

The random match probability of this profile is the probability of getting the three genotypes together.

Following the product probability rule and assuming that loci are independent from each other, the profile probability, PP, is

PP = P₍₁₎ x P₍₂₎ x P₍₃₎ x .... P₍ₙ₎

PP = (P₍₁₀₋₁₄₎ ) (P₍₃₀₋₃₀₎) (P₍₃₋₆₎)

PP = 0.079 x 0.048 x 0.225

PP = 0.000853

The random match probability of this three-locus profile is 0.000853.

To fill in the blank, you need to consider the population size -N- and the probability of getting this three-locus profile.  

Since I do not have the total number of individuals in the population, I will say that my population size, N = 500 individuals and I will make the calcs according to this number. You should follow the same procedure but use your N.

We know that the probability of getting this three-locus profile is 0.000853. This is the frequency of individuals carrying this genotype in the population.

To get the number of individuals with this genotype in the population, we need to multiply this frequency by the total number of individuals, N.

Nº of individuals with the three-locus profile = frequency x N

Nº of individuals with the three-locus profile = 0.000853 x 500

Nº of individuals with the three-locus profile = 0.4265 individuals.

Now we know that in a population of 500 individuals, there are 0.4265 individuals with the three-locus profile. Now we need to know the population size in which we can find 1 individual with this genotype.

0.4265 individuals with the three-locus profile-------- 500 individuals

1 individual -------------------------- X = (1 x 500) / 0.4265 = 1,172.33 individuals.

According to these calculations, approximately one in every 1,172.33 individuals, drawn randomly from the population, would be expected to have this three-locus profile.

Note: Remember to use the population size provided to you in this problem. The procedure is the same, but the population size -N- will be different.

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Can you please help

Answers

Answer:

ggg

Explanation:

would would would would would would would would would would would would would

Answers

Would would would would would

Expain why the lettuce irrigated with a mixture of water and fertilizers grows on gravel​

Answers

A fertiliser, often referred to as plant food, is a broad term for either a mixture of chemicals or naturally occurring matter that is used for enhancing the growth of plants.

When a human steps out into the cold air, the body begins to shiver in order to keep its temperature at 98.6 degrees Fahrenheit.

Answers

Answer:

All living things maintain an internal balance.

Please can you give me a brainiest?

What are zymogens? Give an examples​

Answers

What are zymogens? Give an examples

Inactive precursor of enzymes are known as zymogens. They are also known as proenzyme.

For example:-

Pepsinogen is the precursor of pepsin. Pepsinogen is inactive until it is released by chief cells into HCl. The latter partially activates pepsinogen.

The reproductive system in both males and females are controlled and regulated by the interaction of hormones from the hypothalamus and pituitary glands with hormones from the reproductive organs. How do these hormones affect the development of male and female reproductive systems? (Provide an answer of minimum of 25 words) ​

Answers

How do these hormones affect the development of male and female reproductive systems?

These harmones help both male and female to develop sexual characteristics and play an important role in menstrual cycle, fertility and pregnancy in females. In males, these hormones help make sperm in testes...

What are some ways an area can undergo reclamation after being mined?

Answers

Answer:

Refilled/reshaped w/vegetation planted; filled w/water to become a lake; turned into landfill; left open as home for bats or sealed off if underground.

Klein first became fascinated with crows at what event?
1 His wedding
2 An outdoor concert
3 A marathon
4 A cocktail party

Answers

Answer:

the answer is 4.) a cocktail party

Define the term Fuse

Nira here :)

Answer ASAP pls.....​

Answers

(of an electrical appliance) stop working when a fuse melts.

Come on anyone help please I need this to be done by tomorrow. please ​

Answers

Answer:

1400

Explanation:

Not sure though

subtract

urine=kidneys 1500

sweat= skin. 100

The reproductive system in both males and females are controlled and regulated by the interaction of hormones from the hypothalamus and pituitary glands with hormones from the reproductive organs. How do these hormones affect the development of male and female reproductive systems?​

Answers

I have attached the screenshot since it contained some rude words!!~

What is different between your own kidney and a donated kidney?

CAN SOMEONE PIEASE ANSWER THIS

Answers

Answer:

that its not the kidney you were born with

Explanation:

no explanation

Answer:

Your kidney has different DNA than if you got one from a donor, if that's what you mean

Explanation:

Precipitation occurs when _____.​

Answers

Answer: occurs when a portion of the atmosphere becomes saturated with water vapor , so that the water condenses and "precipitates" or falls.

Hope this helps!

Describe eggs laid in water.

Answers

Answer:

Eggs laid in water? Wet eggs? im not sure i understand? like frog eggs?

Explanation:

if its frog eggs then small and slimy and kind of squishy.

In an
ecosystem, energy is transferred from one organism to another. Which of the
following best shows the transfer of energy among organisms in an ecosystem's
food web?
A. omnivore carnivore - herbivore → - producer
B. producer – herbivore - carnivore decomposer
-
C. producer – decompoder herbivore — Omnivore
D. carnivore ——herbivore decomposer —> omnivore
-

Answers

Answer:

producer – herbivore - carnivore decomposer.

Explanation:

hope it helps you<3

Energy from an earthquake travels
A.
fastest through the gases of Earth's atmosphere.
B.
as light waves that warm the rock underground.
C.
as seismic waves that cause matter to vibrate.
D.
at the same speed through solids, liquids, and gases.
Reset

Answers

Answer:

Energy from an earthquake travels: C. as seismic waves that cause matter to vibrate.

Explanation:

The energy of an earthquake travels in the form of waves. These waves are referred to as seismic or earthquake waves. Earthquake waves are classified into two types: body waves and surface waves. Body waves move through the Earth's interior, whereas surface waves travel along the Earth's surface.

Summarize how Dr. Temple Grandin changed the face of animal husbandry.
need written response

Answers

Answer:

Dr. Temple Grandin changed the face of animal husbandry by creating a new, humane, and efficient way of processing meat. There are even vet clinics inspired by her to be 100% discomfort-free.

Explanation:

Hope this helps!

Dr. Temple Grandin, an American professor of animal science and a prominent advocate for animal welfare, has had a transformative impact on animal husbandry.

Despite facing challenges with autism, she leveraged her unique perspective and deep empathy for animals to revolutionize the way livestock is handled and treated in the agricultural industry.

Dr. Grandin is renowned for her innovative designs of livestock handling facilities that prioritize the welfare of animals. By understanding the sensory sensitivities of animals, she created more humane systems that reduce stress and fear during handling and transportation. Her designs include curved chutes and non-slip flooring, which encourage a calmer movement of animals, minimizing injuries and distress.

Dr. Temple Grandin's contributions to animal husbandry have been instrumental in promoting compassionate practices, reducing animal suffering, and fostering a greater understanding of the emotional needs of livestock. Her groundbreaking work continues to inspire positive change and serves as a testament to the potential for empathy and innovation to transform industries for the betterment of both animals and humans.

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Which of the following are subatomic particles? Select all that apply.
A. Gas
B. Elements
C. Protons
D. Electrons
E. Mixtures
F. Neutrons

Answers

Answer:

Protons, Electrons, and Neutrons are what atoms are made of. Therefore, they're subatomic (smaller than atom) particles.

What is a reflex?

Behavior that does not involve What is a reflex?

Behavior that does not involve the forebrain, or "higher" centers of an animal's
brain.

Similar nerve cells grouped together in a nervous system.

Answers

as a noun one response to the entire physiological process activating such a movement free any automatic I’m thinking often habitual behavior or response or reflection or image of an object as exhibited by a mirror or the like

What is the mRNA sequence for the template strand of DNA if it has the sequence 3’ TACAAACTAGAA 5’
a. 5’ ATGTTTGATCTT 3’
b. 5’ AUGUUUGAUCUU 3’
c. 5’ TACAAACTAGAA 3’
d. 5’ UACAAACUAGAA 3’

Answers

B

Remember,
Adenine goes with Thymine, but in RNA thymine “turns” into uracil

Select the correct locations on the image.
A high gradient in air temperature and pressure can cause a jet stream, characterized by high-speed winds. Through which marked regions
would jet streams pass?
Polar cell
609
Ferrel cell
30
رررررد
Hadley cell
Hadley cell

Answers

Answer:

all boxes on the black lines

Explanation:

there's 4 boxes you should have selected i just took the same test

How much oil was removed?

Answers

Answer:

40ml

Explanation:

90-50=40

PLEASE HELP I NEED BEFORE 11:59

Answers

Answer:

1) Interphase.

2) Prophase I.

3) Metaphase I.

An organism that contains genes from another organism of a different species is a ? Check all that apply
A. Complimentary Organism
B. GMO
C. Mutant
D. Transgenic Organism
E. Translated Organism

Answers

THE ANSWER IS A HOPE THIS HELPS

What is TRUE about using bullet comparisons to solve crimes?

A. A Chinese detective did this in 1248.
B. Francis Galton did this in 1892.
C. A Scotland Yard investigator did this in the 1830s.
D. Mathieu Orfila said that this would never be accurate.

Answers

Answer:

C. A Scotland Yard investigator did this in the 1830s.

Reason:

In the 1830s, a Scotland Yard detective was among the first to utilize bullet comparisons to capture a culprit.

A Scotland Yard investigator did this in the 1830s. Therefore, option C is correct.

What are bullets?

A bullet is a kinetic projectile that is fired from a gun barrel as part of firearm ammunition. The majority of handgun bullets are formed of a lead-antimony alloy contained in a soft brass or copper-plated soft steel jacket, although they can also be made of copper, lead, steel, polymer, rubber, and even wax.

A softer lead core is enclosed in a tougher jacket made of steel or cupronickel in rifle and machine gun bullets. Bullets that penetrate armor have an inner core made of hardened steel.

Thus, a Scotland-yard investigator did this in the 1830s. This is true about using bullet comparison to solve crimes. Therefore, option C is correct.

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How does the cell cycle play into organ donation?

Answers

Answer:

Steps in the process are as follows:

1. Identification of the Potential Donor by the Hospital. ...

2. Evaluation of Donor Eligibility. ...

3. Authorization for Organ Recovery. ...

4. Medical Maintenance of the Patient. ...

5. Matching Organs to Potential Recipients. ...

6. Offering Organs Regionally, Then Nationally. ...

7. Placing Organs and Coordinating Recovery.

Characteristics for forlorn

Answers

Answer:

desolate or dreary; unhappy or miserable, as in feeling, condition, or appearance.

lonely and sad; forsaken.

expressive of hopelessness; despairing: forlorn glances.

bereft; destitute: forlorn of comfort.

Explanation:

hope this helps

Answer:

Desolate or dreary, lonely and sad, forsaken, hopelessness, despairing, forlorn, and destitute.

Explanation:

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