You decide to focus first on Mutation 1 and Mutation 2.
The F' plasmid appears to "rescue" mutant strain 1 by restoring wild-type inducible expression.
The F' plasmid does not change the phenotype of mutant strain 2; there is still no expression of the lac operon.
There are three possible mutations that could lead to lack of expression: a super-repressor (Is), a promoter mutation (P-) or a mutation in the lacZ gene (Z- ). To determine the effect of introducing a wild-type F' plasmid into a bacterial cell with each of these mutations, you draw out the three possible scenarios.
F' l+ cap+
Determine the expression of the lac operon for each possible partial diploid and for the "unknown" mutation partial diploids (Mutation 1 and Mutation 2).
1. F' lac+/ ts
2. F' lac+/ P
3. F' lac+/ Z
4. F' lac+/ Mutation 1
5. F lac+/ Mutation 2
A. Inducible (wild type)
B. No expression (mutant)

Answers

Answer 1

Answer:

Super-repressor (Is) - Uninducible dominant

Binding region is modified by the protein called repressor which prevents the lactose to bind with it. Thus, even the lactose is present the repressor protein is bound to the promoter and can not be detach from it. Transcription is not inducible.

Promoter mutation (P)- - Uninducible recessive

Mutated promoter region inhibits the binding of RNA polymerase that leads to trranscription.

The lacZ gene ( Z-) is mutated - recessive while the normal LacZ gene (Z +) -  dominant .

                   Inducible (Wild type)        No expression (Mutant)

F' lac+/ Is             No                                Yes

F' lac + / P-            Yes                                 No

F' lac + / Z-            Yes                                 No

F' lac +/ Mutation1     Yes                                 No

F' lac+ / Mutation2      No                                 Yes

Mutation 1 and F' plasmid - Inducible wild type

Mutation 2 and F' plasmid - Mutant no expression


Related Questions

In pea plants there is a dominant allele (A) for green pods and a recessive allele (a) for yellow pods. Suppose a heterozygous plant is crossed with a plant that has yellow pods. Complete the sentences about this cross with the correct terms.
1. The phenotype of the heterozygous plant is_________.
2. The genotype of the heterozygous plant is_________.
3. The genotype of the plant with yellow pods is________.
4. The genotypes of gametes produced by a heterozigous plant is______.
5. The genotypes of gametes produced by a yellow plant is_______.
A. Green pods
B. 75% AA and 25% Aa
C. AA
D. 100% A
E. Yellow pods
F. 50%
G. 25%
H. 100% a
I. 50% A and 50% a
J. Aa
K. 0%
L. 75%
M. 75% A and 25% a

Answers

Answer:

1. Green

2. Aa

3. aa

4. A and a

5. a and a

Explanation:

1. The phenotype of the heterozygous plant (Aa) would be green since the allele responsible for green color (A) is dominant over the allele responsible for yellow color (a).

2. The genotype of a heterozygous plant would be Aa. Heterozygous individuals have different alleles for the same gene.

3. The genotype of the plant with a yellow pod would be aa. Since the allele for green color (A) is dominant over the allele for yellow color (a), the yellow color can be expressed only in the absence of the (A) allele. Hence, the genotype fo yellow color has to be aa.

4. The genotypes of gametes produced by a heterozygous plant would be (A) and (a) because heterozygous individuals have two different alleles for a gene.

5. The genotypes of gametes produced by a yellow plant would be (a) and (a) because a yellow plant has the genotype (aa).

Centrioles have pulled the chromosomes all the way to the spindle poles, the process of cleavage furrowing appears to be about to begin. What kind of a cell is this?

Answers

Answer:

An animal cell in the telophase

Explanation:

Telophase is one of the stages of cell division in animal cell .

In the animal cell during telophase, Centrioles have pulled the chromosomes all the way to the spindle poles, the process of cleavage furrowing appears to be about to begin because the nuclear membrane and nucleoli reform, cytokinesis is almost coming to completion and the chromosomes eventually uncoil to chromatin. Usually cytokinesis occurs during telophase.

Which part of the cell functions to recognize other cells? (1 point)
O nucleus
O cytoplasm
O cell wall
O plasma membrane

Answers

Answer:

cell wall maybe it may be wrong also

Plasma membrane of the cell functions to recognize other cells. Thus option D is correct.

What is Plasma membrane?

The plasma membrane which is an outer covering of cell present in both prokaryotic and eukaryotic cell.

It is a thin selectively semi-permeable membrane which enclose the cytoplasm along with other organelle.

Proteins and lipids are the major components of membrane, beside this carbohydrate are also present in cell membrane.  

The most common lipid in membrane is phospholipid, other lipids are glycolipid, sphingolipid.  

The function of cell membrane is to provide protection and maintain the integrity of the internal environment of the cell,

It act as a base of attachment for the cytoskeleton  for cell-cell communication.

It involve in transport of materials like nutrients, toxic substances.

Thus option D is correct.

Learn more about plasma membrane, here:

https://brainly.com/question/14727404

#SPJ2

explain why germinating seeds were used in this investigation cellualr respiration

Answers

Explanation:

As oxygen is consumed to provide energy, germinating seeds release carbon dioxide. ... HYPOTHESES: The experimental hypothesis is that germinating seeds will show a greater rate of respiration than control glass beads. Additionally, that at higher temperatures, the rate of cellular respiration in the seeds will increase.

Formula, name, and Group number of element needed for:     (i) hypothyroidism -     (ii) hypertension     (iii) kidneys     (iv) bones

Answers

Answer:

I) Hypothyroidism involves the insufficient production of thyroid in the thyroid gland which helps in the development of individuals. The element Iodine enhances the production of the thyroid.

I2- Iodine - Group number 17

Hypertension - Too much Salt which contains sodium in foods causes hypertension.

Na- Sodium- Group number 1

Bones require Calcium for its proper development and also helps to strengthen it.

Calcium - Ca- Group number 2

Kidney - Excess sodium in kidneys could result to kidney diseases which is why plenty of water should be taken to help the kidney from this element.

Na- Sodium- Group number 1

which climate does sparrow live hot or cold

Answers

Answer:

Hot ok please give me brilliance

Explanation:

please

The functions of the oral cavity include all of the following except Select one: A. mechanical processing of food. B. absorption of monosaccharides. C. digestion of carbohydrates. D. lubrication. E. sensory analysis of material before swallowing.

Answers

Answer:

C. Digestion of carbohydrates.

Explanation:

The function of the oral cavity that is not performed by the oral cavity is B) absorption of monosaccharides.

What is oral cavity?

The oral cavity, also known as the mouth, plays a crucial role in the digestive process. Its main functions include mechanical processing of food by chewing and grinding, sensory analysis of material before swallowing through taste buds, and lubrication of food through the production of saliva.

The oral cavity also begins the process of digestion by breaking down carbohydrates with the help of enzymes in saliva. Additionally, the oral cavity serves as the entry point for air during breathing and speech. Proper oral health, including brushing, flossing, and regular dental check-ups, is important for maintaining overall health and wellness.

Learn more about oral cavity, here:

https://brainly.com/question/4983902

#SPJ6

what is the key to the recognition of a trait whose expression is determined by the effects of two or more genes

Answers

Answer:

Explanation:

Polygenic inheritance occurs when many loci of a gene contributes to it genetic make up.

No particular allele can be selected for contributing to a particular traits all the allele are equally expressed.

Over expressitivity example is pleiotrophy effect have more than 5 fingers or toes

The offspring express more dominant traits that the normal mendelian principle

This signs indicate polygenic inheritance.

what is a radical a group of ions

Answers

Answer:

A radical is a group of atoms of elements carrying a charge. radicals or ions are formed by gaining or loosing ions. positive radical ions are called radical cations whereas negative radical ions are called radical  anions.

hope this helps you u :)      

If a cell membrane were completely permeable to all substances, could the cell continue to live? Explain your answer in depth.

Answers

If the membrane were fully permeable to all substances, then anything could enter the cell. This would upset the balance between the cell's contents and the outside environment. There is only so much ability to store substances and utilize substances, therefore, the cell would not be able to maintain homeostasis.

What part of the brain is known as the pleasure center?

A. Brain stem

B. Hypothalamus

C. Thalamus

D. Midbrain

SUBMIT

Answers

Answer:

B. Hypothalamus

Explanation:

The hypothalamus contains the pleasure center of the brain, the nucleus accumbens. The hormone dopamine is released from this part of the brain in response to or anticipation of pleasurable activities such as eating good food, monetary rewards as well as when taking psychoactive drugs.

The release of dopamine affects other feelings such as happiness, focus, being alert as well as staying motivated. it also affect some voluntary actions as well.

Constantly seeking out activities that stimulate dopamine release may lead to addiction to such activities as in drug addiction.

explain what perlemoen are?​

Answers

Answer:

Also known as "abalone" which is a

Explanation:

Answer:

any of various edible marine gastropod mollusks of the genus Haliotis

Explanation:

hope this helps

Butterflies can produce hundreds of offspring per cross. In a certain variety of butterflies, a maternally-imprintable gene is responsible for wing phenotype. Two possible alleles for this gene are w for normal wings and wc for crinkled wings. Which of the following pieces of information would be helpful in predicting the wing phenotype of a male offspring from a cross?

a. look at the father's phenotype
b. know the mother's genotype
c. look at the mother's phenotype
d. know the father's genotype

Answers

Answer: d. know the father's genotype

Explanation: Butterflies can produce hundreds of offspring per cross. In a certain variety of butterflies, a maternally-imprintable gene is responsible for wing phenotype.

The option that would be helpful in predicting the wing phenotype of a male offspring from such a cross would be to look at the father's phenotype.

Imprinted genes can simply be defined as genes that are expressed in parent-of-origin specific manner and result from a chemical modification of DNAs.  Such genes are differentially expressed depending on which of the two parents they are inherited from. Hence:

maternally-imprintable genes are only expressed when they are inherited from the father. paternally-imprintable genes are only expressed when inherited from the mother.

Phenotype refers to the physical appearance or expression of a gene. Therefore, if a gene is maternally imprintable, looking at the phenotype of the father would be helpful in predicting the phenotype of the male child in case a cross happens.

Applying the same principle to the butterfly, it will, therefore, be helpful to look at the father's phenotype in order to predict the wing phenotype of a male offspring from a cross.

The correct option is A.

More about imprinted genes can be found here: https://brainly.com/question/7207412

Help anyone please????!

Answers

1. Wavelength
2. Compression
3. Rarefractiom
Please mark brainliest

use the numbers 12345 to place the protien creations steps below in the correct order

Answers

Use the numbers 1, 2, 3, 4, and 5 to place the protein creation steps below in the correct order. Ribosome attaches to the mRNA. Information is transcribed in DNA to mRNA. tRNA anticodon carries an amino acid that compliments the mRNA codon. mRNA leaves the nucleus. The chain of amino acids forms a protein.  

if it is helpfull please mark as brainlist

Answer:

please list numbers 12345 and then i will

Explanation:

a body may have zero velocity even though its speed is 10m/s. give reason.​

Answers

Explanation:

because speed is time rate at which an object is moving along a bath, while velocity is the rate and direction of object's movement .

Vị trí các cacbon trong cấu trúc của đường đềôxyribô trong 1 nuclêôtit được thêm dấu phẩy vì:

Answers

Answer:

hi please answer my questions.

The diagram shows a phase of mitosis. A phase of mitosis is shown. The chromosomes lined up at the center. Letter A represents long strings inside of the cell. Which does the letter A represent in the diagram?

Answers

I wish you added the diagram but I can tell that the long strings are the spindle fibres.

Answer:

the long string things are the fiber spindle thing

( sorry i'm not good at reading passages and citing from them... )

Which of the following small GTPases are NOT involved in vesicle budding or docking? A. ARF B. Rab1 C. Ras D. Sar1p

Answers

Answer:

option c is correct that is Ras

Explanation:

Which is NOT a function of magnesium in the body?
znutritio acting as a cofactor for enzyme systems in the Irody
regulating bone and mineral status
protecting teeth from acid-forming bacteria
making up one component in bone mineral​

Answers

Answer: I think it is the third one which states, "Protecting teeth from acid-forming bacteria".

Explanation:

Saprobic microorganisms are important decomposers of plant litter, animal matter, and dead microbes. This is an example of a(n) ______________.

Answers

Answer:

The correct answer would be - heterotrophs (chemoheterotrophs)

Explanation:

Heterotrophs are the living organisms that are directly or indirectly depend on the autotrophs or the organisms that can make their own food for energy and growth.

Saprobic microorganism are free living organisms that get their energy from decomposing rotten or dead organic matter such as plant litter, animal matter and dead microbes. It is performed with the help of enzymes or chemicals they release to decompose such matter. they also known as chemoheterotrophs.

Thus, the correct answer would be - heterotrophs (chemoheterotrophs).

b) How will you describe any three (3) major components of the environment to a named
class puyil?​

Answers

Answer:

Hydrosphere, atmosphere and biosphere are the three major components of the environment.

Explanation:

Hydrosphere, atmosphere and biosphere are the three major components of the environment. hydrosphere refers to water bodies such as ocean, sea, ponds and lakes etc that is present in our environment. atmosphere refers to the gaseous layer which is present above the earth surface. in this layer oxygen, nitrogen and carbondioxide etc are present. biosphere refers to all living organisms such as human, animals, plants and microbes etc which are present on earth surface..

Describe how scent and taste work in conjunction. Are the tastes of below mention foods that were tested heightened by the sense of smell, or only some of the foods?

a. Salt
b. Sugar
c. Lemon Juice
d. Coffe grounds

Answers

Answer:

Scent and Taste work in conjunction because when your brain smells food, it pulls up a picture or memory of that food in your head, and you remember what it tasted like and if you enjoyed it.

Only some of the food. Salt does not really have a smell, so it does not do anything, but all the others do and are heightened

Explanation:

Take cotton candy for example. When you smell cotton candy, you smell SUGAR and lots of it. You know that sugar tastes good and as such you now want to eat the cotton candy.

For this example, we will use lemonade. Lemonade is commonly a drink that most love on a hot summer day at the beach. When you smell the lemonade, it reminds your brain of how good it tasted and pulls up the memory at the back of your mind,  making you feel happy and relaxed. It then ends up tasting better

(This is my first answer, it won't be perfect)

Smell and taste go hand in hand because when you smell food, your brain conjures up an image or recollection of that food in your head, allowing you to recall how it tasted and if you liked it.

What is Conjuction?

Since salt doesn't truly have a fragrance, it has no effect; nevertheless, all the others do and their intensity is increased.

You can definitely smell a lot of sugar when you smell cotton candy. You want to eat the cotton candy because you are aware that sugar tastes delicious.

On a hot summer day at the beach, most people enjoy drinking lemonade.

Therefore, Smell and taste go hand in hand because when you smell food, your brain conjures up an image or recollection of that food in your head, allowing you to recall how it tasted and if you liked it.

To learn more about conjuction, refer to the link:

https://brainly.com/question/25713213

#SPJ2

Which scientist was responsible for coining the phrase "Big Bang"? *
O Fred Hoyle
O Albert Einstein
O Sir Isaac Newton
O Edwin Hubble

Answers

Answer:

Fred Hoyle

Explanation:

Sir Fred Hoyle FRS was an English astronomer who formulated the theory of stellar nucleosynthesis. He also held controversial stances on other scientific matters—in particular his rejection of the "Big Bang" theory, a term coined by him on BBC radio, and his promotion of panspermia as the origin of life on Earth

The scientist that was responsible for coining the phrase “Big Bang” was
a) Fred Hoyle

Choose the PCR primer pair that will amplify the breakpoint of a deletion of the segment of DNA between lines 1and 2.
5' TCGATTCCGGAAAGCTTAGTTTCCCGGGACGTATTGCCAACCTAGGTAAGCGCCGAATATCCATGGGCACC 3'
3' AGCTAAGGCCTTTCGAATCAAAGGGCCCTGCATAACGGTTGGATCCATTCGCGGCATTATAGGTACCCGTGG 5'
a. 5- AGCTAAGGCCTTTCGA and 5'-CCACGGGTACCTATAA
b. 5'- TCGATTCCGGAAAGCT and 5'-ACGTCCCGGGAAACTA
c. 5- TCGATTCCGGAAAGCT and 5'-CCACGGGTACCTATAA
d. 5'- TCGATTCCGGAAAGCT and 5'- GGTGCCCATGGATATT
e. 5' - GGTGCCCATGGATATT and 5' ACCTAGGTAAGCGCCG

Answers

Answer:

a. 5- AGCTAAGGCCTTTCGA and 5'-CCACGGGTACCTATAA

Explanation:

The Polymerase Chain Reaction (PCR) primers are short nucleotide sequences (composed of approximately 20  nucleotides in size) flanking a target sequence that is amplified during PCR reaction. These primers bind to the DNA template by means of complementary base pairing in order to make billions of copies of a target DNA region, which is then visualized as a band by electrophoresis. In this case, PCR primers from the item a- (i.e., AGCTAAGGCCTTTCGA and CCACGGGTACCTATAA) will bind to the DNA template of lines 1 and 2 in order to amplify a continuous region:

Schematically:

The Foward primer AGCTAAGGCCTTTCGA binds by complementary base pairing:

5'_(TCGATTCCGGAAAGCT)TAGTTTCCCGGGACGTATTGCCAACCTAGGTAAGCGCCGAATATCCATGGGCACC_3'

The Reverse primer CCACGGGTACCTATAA binds by reverse complementary base pairing:

3'_AGCTAAGGCCTTTCGAATCAAAGGGCCCTGCATAACGGTTGGATCCATTCGCGGCA(TTATAGGTACCCGTGG)_5'

What is the process of
evaporation?

A. The process in which plants release vapor into
the atmosphere

B. Any process that returns water from the
atmosphere to the earth

C. The process in which liquid water turns to
water vapor

Answers

The answer to your question is C

You have been assigned a DNA stretch. What is the complementary strand when you replicate the template

Answers

Answer:

The complementary strands of a DNA are read in the opposite direction to one another

Explanation:

A DNA strand/stretch can be represented with a number of bases. The bases in a DNA strand are Adenine (A), Thymine (T), Cytosine (C) and Guanine (G). The Adenine can bind to Thymine (and vice-versa) while the Guanine can bind to Cytosine (and vice versa). These bonded bases are called "base pairs".

Complementary DNA strands are read in opposite direction to one another. Each end of a DNA strand is either a 5' end or a 3' end. Hence, if one strand runs from the 5' to 3' end, the complementary strand will run from the 3' to 5'. Thus, they are represented "on paper" the way they run.

For example,

If the DNA strand assigned is AGTCTAG running 5' to 3', the complementary strand will be CTAGACT (starting from the 3' end) and NOT TCAGATC (which starts from the 5' end)

ASAP When ______ is hydrolyzed, it forms _______. A. protein, amino acids B. ATP, ADP C. polysaccharide, monosaccharide D. lipid, triglyceride

Answers

Answer:

The answer is ATP, ADP

Explanation:

When protein is hydrolyzed, it forms amino acids. Thus, the correct option is A.

What is Hydrolysis?

Hydrolysis may be defined as a chemical process that utilizes the molecules of water that involve the chemical breakdown of a compound.

Amino acids are the monomers of protein. These amino acids are linked together by peptide bonds. But the process of hydrolysis breaks the peptide bond between the amino acid sequences and released them significantly in free form.

Therefore, when protein is hydrolyzed, it forms amino acids. Thus, the correct option is A.

To learn more about Hydrolysis, refer to the link:

https://brainly.com/question/4352413

#SPJ5

The correct sequence for western blotting is:

1. Proteins are denatured by sodium dodecyl sulfate (SDS), and by boiling.
2.Transfer proteins to the nitrocellulose membrane.
3. Performing the polyacrylamide gel electrophoresis (PAGE).
4. Incubate the nitrocellulose membrane to primary antibodies and secondary antibodies subsequently.

a. 1,2,3,4
b. 4,1,3,2
c. 1,3,2,4
d. 1,3,2,4

Answers

Answer:

3.page is this that answer

Explanation:

a.1,2,3,4

The correct sequence for western blotting is: C. 1,3,2,4

Blotting can be defined as an analytical technique that is designed for the analysis of biological macromolecules such as:

Lipids.Nucleic acid.Proteins.Carbohydrates.

For western blotting, the correct sequence of analyzing biological macromolecules such as proteins is:

1. The denaturation of protein through the use of sodium dodecyl sulfate (SDS) and by boiling.

2. The performance of polyacrylamide gel electrophoresis (PAGE).

2.The transfer of proteins to the nitrocellulose membrane.

4. Lastly, the incubation of the nitrocellulose membrane to subsequently form primary antibodies and secondary antibodies.

Read more: https://brainly.com/question/14681125

Apoptosis differs from necrosis in that necrosis:_______ a. requires the reception of an extracellular signal. b. causes DNA to fragment. c. causes cells to swell and burst, whereas apoptotic cells shrink and condense.d. involves a caspase cascade

Answers

Answer:

c. causes cells to swell and burst, whereas apoptotic cells shrink and condense

Explanation:

These two mechanism are responsible for death of cells in multi cellular organisms.Apoptosis is  naturally occurring process which is well organised and programmed.It is ensures the  automatic death of old and worn out cells, needed for certain cellular developments. This is demonstrated by DNA fragmentation,cell shrinkage,and the break down of the nuclear walls.It can be initiated through two pathways of intrinsic and extrinsic,The latter leads to death from internal factors,while in the former is when external factor causes the death .

However,when cell death is influenced by external factors(toxins)  in the cell environments,which lead to unpredictable or unregulated process leading to cell death ,the process is called Necrosis.it is demonstrated with the swelling and busting of the cell.and characterised with inflammation. It is an accidental death which causes injuries to the cells and tissues.

Other Questions
The equations y=x228 and y=2x2 are graphed below. What are the solutions to the equation x228=2x2?A x=8 and x=2B x=6 and x=10C x=4 and x=4D x=2 and x=6 )There are 120 mangoes in a basket and 168 mangoes in another basket.Find the greatest number of mangoes which are to be taken out at a timefrom each basket so that both of them will be emptied simultaneously. change the following word equations into balanced equationsa) Hydrogen + Chlorine Hydrogen chloride.b)Hydrogen + Nitrogen Ammoniac)Aluminium + sulphuric acid Aluminium sulphate + Hydrogend)Iron (3) + oxygen iron oxidee)Copper + sulphuric acid Copper sulphate + water + sulphur dioxide. 3. The International Monetary Fund (IMF) and the World Bank are two institutions that have outlived their usefulness. Do you agree? Venn diagrams: unions, intersections, and complementsAttached is the photo reference After analyzing its own resources and unique abilities, a company is now trying to determine what group of customers it can satisfy with a good or service. It is in the process of choosing a Life Cycle of a Frog A student received the following grades: An A in Statistics (4 credits), a B in Physics II (5 credits), and a D in Tennis (1 credit).Assuming A = 4 grade points, B = 3 grade points, C = 2 grade points, D = 1 grade point, and F = 0 grade points, the student's grade point average is: Q/A and Fill in the blanks What is seven to the fifth power equal to 200% of 85 is what number What is the yield to maturity of a bond that pays a 6% coupon rate with semiannual coupon payments, has a par value of $1,000, matures in 15 years, and is currently selling for $803 How did the British respond to the Albany Plan of Union? They opposed it because they worried that colonists would support the French. They supported it because they wanted colonial military help. They supported it because they wanted the colonies to be united. They opposed it because they did not want the colonies to be united. if you......starting a new company, how could you raise money? A.are B. was C. had been D. were Which of the following best describes how crossing over occurs during meiosis? During the year, Wright Company sells 500 remote-control airplanes for $110 each. The company has the following inventory purchase transactions for the year. Date Transaction Number of Units Unit Cost Total Cost Jan. 1 Beginning inventory 60 $66 $3,960 May. 5 Purchase 280 69 19,320 Nov. 3 Purchase 230 74 17,020 570 $40,300 Calculate ending inventory and cost of goods sold for the year, assuming the company uses FIFO. 1.The Springfield Meteorological Center wanted to determine the accuracy of their weather forecasts. Theysearched their records for those days when the forecaster had reported a 70% chance of rain. Theycompared these forecasts to records of whether or not it actually rained on those particular days. Theforecast of 70% chance of rain can be considered very accurate if it rained on:A. 95% to 100% of those days.B. 85% to 94% of those days.C. 75% to 84% of those days.D. 65% to 74% of those days.E55% to 64% of those days. If a wheel has a radius of 5cm how much is one rotation of the wheelHow many rotations can the wheel do within a distance of 50km Which type of wetland includes a mixture of salt water and fresh water? Review what you know about products and sums represented by rectangular area models. [5 points] Use algebra tiles to multiply (x-1)(3x+2).