You put 10ul (one loopful - the loop is calibrated for this volume) of a 0.03 ug/ul pGLO solution into the DNA tube. Calculate the ug of DNA you put into the tube.

Answers

Answer 1

Answer:

The µg of DNA put into the tube is 0.3 µg.

Explanation:

Here in this question, we are interested in calculating the µg of DNA put into the DNA tube.

This can be calculated using a mathematical formula.

Mathematically;

Total amount of DNA in µg = concentration in µg/ µl X volume in µl

= 10 µg/µl X 0.03µl = 0.3 µg.


Related Questions

Mitotic cell division creates identical copies by replicating a cell's DNA __________ and then dividing ____________.

Answers

Answer:

Mitotic cell division creates identical copies by replicating a cell's DNA once and then dividing once.

What test would be performed by the hospital to determine a diagnosis? List them

Answers

Answer:

1 pet scan

2 x ray

Explanation:

hope it helps you

Animal migration on savannas is often governed by

distance
reproduction
rainfall
fire

Answers

Answer:

The answer would be rainfall.

Explanation:

The reason why this is the answer is because rain is essential for survival in such a hot and dry climate. Droughts are quite frequent in the savanna and the lack of water leads to the death of many organisms.

Answer:

Rainfall is what often governs animal migration on savannas.

Explanation:

Brainlies please

Role of mitosis in plant growth and development

Answers

Answer:

The mitosis cell division helps in vegetative growth in plants. It also helps in healing of wounds

You continue this approach by designing two separate experiments. In the first experiment, you use glucose where "Coccupies position 1. In the second experiments, 14C occupies position 2 and 6 in the glucose molecules. For each experiment, you use 0.2 moles of radiolabeled glucose and you assume that all the pyruvate formed is converted to acetyl-CoA. What following statements are correct? (select 3)
a) When glucose is labeled on carbon #1, 0.2 mole of acetyl-CoA is radiolabeled
b) When glucose is labeled on carbon #1, 0.1 mole of acetyl-CoA is radiolabeled
c) When glucose is labeled on carbons #2 and #6, 0.2 mole of acetyl-CoA is radiolabeled
d) When glucose is labeled on carbons #2 and 6, 0.1 mole of acetyl-CoA is radiolabeled
e) When glucose is labeled on carbons #2 and 6, the carbonyl groups of half of the acetyl-CoA molecules are radiolabeled
f) When glucose is labeled on carbons #2 and 6, the methyl groups of half of the acetyl-CoA molecules are radiolabeled

Answers

Answer:

b) When glucose is labeled on carbon #1, 0.1 mole of acetyl-CoA is radiolabeled

c) When glucose is labeled on carbons #2 and #6, 0.2 mole of acetyl-CoA is radiolabeled

e) When glucose is labeled on carbons #2 and 6, the carbonyl groups of half of the acetyl-CoA molecules are radiolabeled

f) When glucose is labeled on carbons #2 and 6, the methyl groups of half of the acetyl-CoA molecules are radiolabeled

Explanation:

When glucose undergoes glycolysis, it is converted into two molecules of pyruvate with carbon 1 to 3 forming the first molecule of pyruvate and carbon 4 to 6 the second molecule of pyruvate. The C-1 and C-6 of the glucose molecule becomes the methyl groups of each of the two molecules of pyruvate. The C-2 and C-5  of the glucose molecule forms the carbonyl carbon of each of the two pyruvate molecules. Each of the two pyruvate molecules undergoes further oxidation to yield acetyl-CoA with the carbonyl and methyl groups of pyruvate retained in the acetyl-CoA molecules.

Thus when 0.2 moles of glucose are labelled at C-1 and then C-2 and C-6 in each of the two experiments the following results are obtained:

When glucose is labeled on carbon-1, 0.1 mole of acetyl-CoA is radiolabeled  since half of the two pyruvate molecules are obtained from C-1

When glucose is labeled on carbon-2 and carbon-6, 0.2 mole of acetyl-CoA is radiolabeled since the C-2 and C-6 of the glucose molecules forms a part of one of each of the two pyruvate molecules.

When glucose is labeled on carbons #2 and 6, the carbonyl groups of half of the acetyl-CoA molecules are radiolabeled since one of the two carbonyl groups of the two pyruvate molecules is  formed from C-2 of glucose.

When glucose is labeled on carbon-2 and carbon-6, the methyl groups of half of the acetyl-CoA molecules are radiolabeled since one of the two methyl groups of the two pyruvate molecules is  formed from C-6 of glucose.

An sixty-year-old man has painful fluid-filled vesicles that have begun crusting over in a wide band across the left side of his lumbar area. He has a history of chickenpox. Based on this information, the likely disease is which is caused by:________

a. scabies, Pediculosis humanus
b. fever blisters, HSV-1
c. impetigo, Staphylococcus aureus
d. German measles, Rubella
e. shingles, varicella-zoster virus

Answers

Answer: shingles, varicella-zoster virus

Explanation:

It should be noted that shingles usually occur with old people and it causes a painful rash when the chicken pox virus is reactivated in the body.

Since the man is old and has painful fluid-filled vesicles that have begun crusting over in a wide band across the left side of his lumbar area and also has a history of chickenpox, it's very likely it is shingles.

How does non-random mating cause
evolution?

A. A population that ensures each individual reproduces
causes the population to evolve.
B. When multiple species mate with each other the
population evolves.
C. Individuals choose their mates, meaning that some
individuals pass on their genes and other do not.
D. Individuals that mate with the same individual every
year help evolve their population.

Answers

A. A population that ensures each indivdual reproduces

Is minerals renewable or non renewable?

Answers

Answer:

Minerals are non renewable if it is fossil......

Answer: Minerals are nonrenewable resources.

Explanation:

A nonrenewable resource is a resource that can not be replenished during a human lifespan. Minerals, metal ores, and fossil fuels are examples of nonrenewable resources.

(06.03 LC) Which of the following is an example of how HIV can be transmitted from one person to another? Contact between infected blood and a mucus membrane Contact between infected saliva and an open wound Contact between infected blood and skin Contact between infected saliva and lining of mouth

Answers

Answer:

i believe the answer is contact between a infected blood and a mucus membrane

Explanation:


Identify the organelle where photosynthesis takes place.

B

C

D

E

Answers

Answer:

E

Explanation:

If E is the Chloroplasts, where photosynthesis takes place, then the answer is E.

Answer:

E

Explanation:

Which of the circumstances below most accurately describes conditions that are likely to permit a robust anti-tumor adaptive immune response?

a. A tumor with high expression of proteins that have mutations in sequences encoding HLA-binding peptides.
b. A robust acute inflammatory response to PAMPs expressed specifically by tumor cells.
c. Anti-tumor T lymphocytes that have high expression of CTLA-4.
d. A tumor with high expression of PD-L1.

Answers

Did you ever figure out the answer to this question?

The study of PAMP-DAMP complexes is vital to the advancement of knowledge regarding inflammatory disorders in general and cancer in particular. Therefore, option (B) is correct.

What is PAMP-DAMP complexes?

Increasing evidence links inflammation to cancer, and at the root of inflammation are PAMPs and DAMPs (DAMPs). Microorganisms contain PAMPs, which are detected by pattern recognition receptors on monocytes and DCs (PRRs). PRR activation produces pro-inflammatory cytokines.

A robust immune response requires endogenous chemicals that pose 'risk' to self-tissues and are created by injured or stressed cells; these are DAMPs, which also trigger inflammation. PAMPs and DAMPs each have about 100 receptors. PAMPs and DAMPs interact; a PRR can bind to both. In this system, PAMPs and DAMPs affect each other's activation threshold. Thus, PAMP-DAMP relationships describe inflammation in a predictable 'inflammatory code'

PAMP-DAMP complexes are key to understanding inflammatory disorders and cancer.

Learn more about adaptive immune response, here:

https://brainly.com/question/28073370

#SPJ5

How carbonic acid formed and what is its role in chemical weathering

Answers

Answer:

Carbonic acid is formed in small amounts when its anhydride, carbon dioxide (CO2), dissolves in water. Carbonic acid is a very common in nature where it works to dissolve rock.

Explanation:

In which of the following ways is DNA replication different from DNA transcription?

Answers

Answer:

Thymine is used in replication while uracil is used in transcription.

Explanation:

New cells can only be created after transcription has taken place. Replication results in mRNA strands while transcription creates new DNA. Transcription happens outside the nucleus and replication happens inside the nucleus. Thymine is used in replication while uracil is used in transcription.

DNA replication is a process whereby DNA makes a copy of itself while DNA transcription is a process whereby a messenger RNA is synthesized from a DNA strand.

Both processes involve the building-up of nucleotides or purine.pyrimidine bases. Replication involves building up of cytosine, guanines, adenine, and thymine bases while in transcription, the thymine base is replaced by uracil base.

Hence, the correct answer is that Thymine is used in replication while uracil is used in transcription.

Can somebody help me

Answers

Answer:

No they should not, heres why:

Explanation:

It has been proven on countless occasions that people forget details within minutes of an event. When you forget things, and you try to remeber, your brain will fill in the blanks with what it wants to see. In every case, if possible try to use hard evidence, ie: Videos, recordings, fingerprints, blood, and other stuff. If not that try to use multiple witness accounts and compare them.

Circle
1
2
Circle the most reactive metal in each set.
1) Magnesium / Potassium
2) Aluminum / Gold
3) Cobalt / Cesium / Calcium
4) Iron / Titanium / Potassium
5) Francium / Lithium / Beryllium
لی

Answers

Answer:

Explanation:

1) potassium

2)Aluminium

3) Cesium

4) potassium

5) Beryllium

The process of diffusion of solvent particles from the region of less solute Concentration to a region of High solute Concentration through semi-permeable Membrane is known as​

Answers

Answer:

The process of diffusion of solvent particles from the region of less solute Concentration to a region of High solute Concentration through semi-permeable Membrane is known as (osmosis)

A decrease in muscular activity or damage to neurons that attach to skeletal muscle can lead to a reduction in the size of muscle called

Answers

Answer:

The correct answer is atrophy

what is haemopoiesis??​

Answers

Haemopoiesis is from greek meaning “ to make. new blood” •

Explanation:

It refers to the formation of blood cellular. components.

The best fossil evidence to suggest that Neandertals could produce a language like that of modern humans comes from which bone(s) collected at Kebara, Israel?
a. the hyoid
b. the mandible
c. the cervical(neck) vertebrae
d. the ribs

Answers

Answer:

The answer is A.

Explanation:

The best fossil evidence that suggests Neandertals' ability to produce a language like modern humans comes from the hyoid bone.

This bone is located in the neck between the chin and the thyroid, which is also named "tongue bone" or "language bone".

So the correct answer is A.

I hope this answer helps.

The change in frequency of bean types over generations demonstrates how natural selection drives evolution by changing allele frequencies within a population.

a. True
b. False

Answers

A.True hdhdhdheshdhhdhdhd
The answer is true change in frequency of bean type over generation demonstrates

The diagram represents a food pyramid. The concentration of the pesticide DDT in individual
organisms at level D is higher than the concentration in individuals at level A because DDT is
A. produced by organisms at level C ingested by
those in level D
B. passed through levels A, B, and C to organisms
at level D.
C. excreted by organisms at level A as a toxic
waste.
D. synthesized by organisms at level D.

Answers

Answer:

The answer is "Choice b".

Explanation:

In the given question diagram is missing. so first, we define the diagram after that we explain why the above given choice is correct.  

In the attached file the food pyramid can be a divide into the level, in which the D pesticides use the "dichloro-diphenyl-trichloroethane ", which concentration is higher than its entities from the level of A because DDT is transferred with species at level D by levels A, B, and C, that's why the choice "b" is correct.  

Which statement best describes a hypothesis?

Answers

Answer:

A hypothesis is an idea or explanation you can test through study and experimentation.

Explanation:

HELP PLEASE FAST ASAP PLEASE HELP

Answers

Answer:

D. Their ability to replicate very fast and natural mutations combine to allow them to evolve rapidly

Explanation:

They develop mutations in their DNA that can be resistant to the drugs given.

Cloning to produce embryonic stem cells is called A) regenerative cloning. B) transplantational cloning. C) reproductive cloning. D) therapeutic cloning. E) dedifferentiation.

Answers

Answer:

D

Explanation:

The correct option would be therapeutic cloning.

First and foremost, cloning refers to the process of producing genetically and phenotypically similar organisms or cells from a single organism/cell, be it naturally or artificially. The genotypically and phenotypically similar copies of the original organism are called clones.

Artificial clonings are of different types, namely:

Reproductive cloningGene cloningTherapeutic cloning

Reproductive cloning has to do with producing genetically identical organisms from a particular organism while gene cloning involves producing exact copies of a gene or segments of DNA. Therapeutic cloning, however, involves the production of embryonic stem cells in order to create tissues that would replace similar but damaged or worn-out tissues in living organisms.

Correct option: D

​"Acidic" is an appropriate description for four of the following. Which one is the exception?

Answers

Answer:

magnesium hydroxide

Explanation:

is the inorganic compound with the chemical formulaMg( OH)2.it is a common component of antacid such as milk of magnesia

What is a factor of nutrient collapse?

Answers

Answer:

The nutrient collapse  that atmosphere is change that food we eat and no is paying attention.

Explanation:

Nutrient collapse in the agriculture research it the mos important foods been getting nutrition less, they generally vegetables, fruits minerals and vitamin, protein content has to past.

The researching choosing crops for higher yields and nutrition and wheat to less nutrient,fruits and vegetables that form protein to calcium, iron and vitamin are varieties choosing to grow.

Atmosphere may be change and food we eat,and human needs a oxygen and they plants need a carbon dioxide, and the level the atmosphere is rising.

Carbon dioxide is the atmosphere in turn the contributes to the plant growth,and food production an quality of food is better.

Human nutrient linked through a quality of plants and affected the balance of nutrient in rice, crop and they people rely on, human chain need to measured and understood.

It simply not discussed to the agriculture and health public or nutrient communities.

Have you ever noticed that aside from zoos, you don't see kangaroos, wombats, and koala bears outside of Australia? This is because there is a trench that is a "biogeographical transition zone between Asian and Australian flora and fauna". What is the name of that trench?

Answers

Answer:

Wallace Line

Explanation:

Wallace Line is a boundary line which is a transitional zone between Asia and Australia. This boundary drawn by the British naturalist Alfred Russel Wallace in 1859. This boundary restricts the transferring organisms such as flora and fauna from Australia to Asia. So that's why kangaroos, wombats, and koala bears are only present in Australia not in the continent of Asia.

I need help, please!!

Answers

Answer:

Aerobic respiration ; Respiration using oxygen

Mitochondria  ; Where respiration takes place

Anaerobic respiration ; Respiration in the absence of oxygen

Lactic acid ; Substance that build up in the muscles during anaerobic respiration .

Fermentation ; Process that converts sugar into alcohol

Explanation:

I hope it helps :)

Answer:

aerobic respiration - resperation using oxygen

mitochondria - where resperation takes place

anaerobic respiration - resperation in the absence of oxygen

lactic acid - substance that builds up in muscles during anerobic resperation

fermentation - process that converts sugar into alcohol

as Ethanol fermentation is also known as alcoholic fermentation

( hope I helped you ) :)

The anticodon (Select all that apply):

a. is a triplet of nucleotides in tRNA
b. determines the identity of the amino acid to be added to the peptide chain
c. is complementary to the codon
d. binds to the codon via hydrogen bonds

Answers

Answer:

choice A

Explanation:

An anticodon is a trinucleotide sequence complementary to that of a corresponding codon in a messenger RNA (mRNA) sequence. An anticodon is found at one end of a transfer RNA (tRNA) molecule.

PLEASE HELP FAST PLEASE ASAP HURRY

Answers

Answer:

A number is a correct answer

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