in the affinity chromatography lab which type of interaction will be utilized to purify lactate dehydrogenase?

The solute potential at 26 degrees Celsius of the molar concentration of a sugar solution in an open beaker has been determined to be 0.5m is -12.4 bar.

Solute potential can be calculated by using:

Ψ = -iCRT

As we know,

i = 1

C = 0.5 (mol/L).,

R = 0.0831 L·bars/mol·K

T = 299K

Ψ = - 0.5*0.0831*299 = - 12.4 bar

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The other ions are S[tex]O^{2-}_{4} }[/tex] and C[tex]O^{2-} _{3}[/tex].

Precipitation reaction:

A chemical reaction occurs in an aqueous solution where two ionic bonds combine, resulting in the formation of an insoluble salt. These insoluble salts formed in precipitation reactions are called precipitates.

The solubility rules to determine the solubility of the compound are as follows:

1. The common compounds of group 1A are soluble.

2. All the common compounds of ammonium ion and all acetates, chlorides, nitrates, bromides, iodides, and perchlorates are soluble in nature. Only the chlorides, bromides, and iodides of [tex]Ag^{+}[/tex] ,[tex]Pb^{2+}[/tex] ,[tex]Cu^{+}[/tex]  and [tex]Hg^{2+} _{2}[/tex]  are not soluble.

3. All common fluorides, except for group 2A fluorides, are soluble. Moreover, sulfates except CaSO4 ,SrSO4 ,BaSO4 ,Ag2SO4  and PbSO4 are soluble.

4. All common metal hydroxides except CaSO4,Sr(OH)2 ,Ba(OH)2  and hydroxides of group 1A and that of transition metals are insoluble in nature.

5. All carbonates and phosphates, except those formed by group 1A and ammonium ion, are insoluble.

6. All sulfides, except those formed by groups 1A, 2A, and ammonium ion are insoluble.

7. Salts that contain [tex]Cl^{-}[/tex] ,[tex]Br^{-}[/tex]  or[tex]I^{-}[/tex]  are usually soluble except for the halide salts of [tex]Ag^{+}[/tex],[tex]Pb^{2+}[/tex]  and ([tex]Hg^{2+} _{2}[/tex]).

8. The chlorides, bromides, and iodides of all the metals are soluble in water, except for silver, lead, and mercury (II). Mercury (II) iodide is water-insoluble. Lead halides are soluble in hot water.

9. The perchlorates of group 1A and group 2A are soluble in nature.

10. All sulfates of metals are soluble, except for lead, mercury (I), barium, and calcium sulfates.

The reaction of Ca2+ and [tex]SO^{2-} _{4}[/tex] is as follows:

[tex]Ca^{+}[/tex](aq)  +  [tex]CO^{2-} _{3}[/tex](aq) --------> CaCO3(s)

Therefore there are [tex]SO^{2-} _{4}[/tex] and [tex]CO^{2-} _{3}[/tex] that can form a precipitation.

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The function of propylene glycol and methyl oleate in a hand cream is as a thickening agent and as an emulsifier and skin softener used in cosmetics.

Propylene Glycol

Propylene glycol is a petroleum derivative product that has no odor and is colorless. This product is one of the products used as a raw material for making cosmetics. The function of propylene glycol is as a thickener for cosmetics such as facial moisturizers, facial cleansers, and other cosmetics that are liquid.

Methyl Oleate

Methyl Oleate is a long-chain saturated fatty acid found naturally in various animal and plant fats. Methyl oleate is solid and white in color, and has a mild aroma and is sometimes crystalline. The content contained in this product is often used as raw material for cosmetic products. The function of methyl oleate is as an emulsifier and lubricant that can soften the skin, and help keep cosmetic products from separating. One important function of methyl oleate in cosmetic products is to create a moist, slippery and unbreakable texture.

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Initial main byproducts of the reaction between concentrated hydro iodic acid and benzyl isopropyl ether are isopropyl alcohol and benzyliodide.

Ethers - The word Ether has been derived from the Latin word ‘aether’ which means ‘to ignite’. They contain an ether group—an oxygen atom connected to two alkyl or aryl groups.

At room temperature and under a high-pressure ethers are usually flammable. The general formula of ether is given as R-O-R, R-O-R’, R-O-Ar or Ar-O-Ar where R represents an alkyl group and Ar represents an aryl group.

The initial stage in the hydrophilic acid cleavage of ethers will be a pre-equilibrium protonation of the ether's oxygen.

The resonance-stabilized benzyl cranium ion and isopropyl alcohol will then be produced when the powerful nucleophile iodide ion displaces isopropyl alcohol from the main group.

It is feasible for the hydroid acid and isopropyl alcohol to react further to produce the specified isopropyl iodide.

Thus, Initial main byproducts of the reaction between concentrated hydro iodic acid and benzyl isopropyl ether are isopropyl alcohol and benzyliodide.

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Iron(II) oxide ions are contain that lost 2 electron in the oxidation process and iron(III) oxide contain iron that lost 3 electron

Ferrous oxide is commonly known as iron(II) oxide contain iron that lost 2 electron in the oxidation process and so it is able to bond with other atom that have an extra 2 electron and ferric oxide is commonly known as iron(III) oxide and it contain iron that lost 3 electron and iron(II) and iron(II) oxide is the chemical compound with formula Fe₃O₄ and it occur in nature as the mineral magnetite and it is one of the number of iron oxide and the other being iron(II) oxide FeO which is rare and iron(III) oxide Fe₂O₃ which also occur naturally as the mineral hematite

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In a catalyzed chemical reaction, one function of a catalyst is to increase the number of successful reactant collisions .

catalyzed reaction are the reaction which used to accelerate the rate of the reaction. Catalyst is used to increases the speed of reaction . catalyst minimize the path of the reaction. the main function of the catalyst is to speed up the chemical reaction. the catalyst is not being consumed during the chemical reaction. catalyst is to increase the number of successful reactant collisions .

Thus, In a catalyzed chemical reaction, one function of a catalyst is to increase the number of successful reactant collisions .

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C2HBrClF3 and O2 have partial pressures of 84 torr and 778 torr, respectively.

Given,

C2HBrClF3 mass = 15.0 g

O2 mass is 22.6 g.

C2HBrClF3's molar mass is 197.4 g/mole.

O2's molar mass is 32 g per mole.

Raoult's Rule

Xsolvent = Psolution

P0solvent

P solution is the solution's vapour pressure.

"solvent" is the solvent's mole fraction.

P0solvent = the pure solvent's vapour pressure

Raoult's Law-

Raoult's law states that the partial vapour pressure of a solvent in a solution (or mixture) is equal to or the same as the pure solvent's vapour pressure times the mole fraction of the solvent in the solution.

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Peroxide is sprayed on all samples before any indicator so as to bleach the samples  before staining by the indicators

peroxide is any of a class of chemicals in which 2 oxygen atoms are linked together by a single covalent bond peroxide is useful in bleaching as they are agents bleaching.

The peroxide when applied to the sample it reduces color bodies in pulp by oxidizing the carbonyl group making the samples colorless.

When the indicator is applied to a sample that is bleached the indicator penetrates this highlights important features of the tissue as well as enhances the tissue contrast.

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Iron metal has a sea of electrons feature. The outside electrons form a sea or pool of delocalized electrons that are free to travel randomly throughout the fixed lattice of positive ions and do not "belong" to any particular atom.

On the Periodic Table of the Elements, iron is a metal that belongs to Group 8 and is a hard, brittle substance. The most common metal, it is pure form, corrodes quickly when exposed into humid air and high temperatures. Iron is also considered to make up a significant portion of the Earth's core and is the fourth most prevalent metal by weight in the Earth's crust. According to the Los Alamos National Laboratory, it is abundant in the sun and stars in addition to being widely distributed on Earth. According to Jefferson Lab, iron is essential for the survival of all living things.

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Sample of an element. At room temperature, it is a lightweight, brittle solid with a metallic luster. It has fair conductivity that increases with increased temperature the material is is metalloid

An element is the simplest form of matter that cannot be split into simpler substances or built from simpler substances by any ordinary chemical or physical method and element are further classified into metal, nonmetal and metalloid and metalloid are all solid at room temprature and it appear lustrous but is not malleable or ductile and it is much poorer conductor of heat than the metal and the material has properties of both metal and non metal that means the material is probably metalloid

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0.00292 mol of HCL would be needed to react with 0.0356 g of Mg.

Mass of mg = 0.0356 g

Molar mass of mg is 24.31 g/mol

moles of mg= 0.0356/24.31

=0.00146moles

Mg+2HCL→MgCl₂ +H₂

From above equation , 1 mol of mg requires 2 mol of HCL

∴ 0.00146 mol of Mg will require =2×0.00146 mol of HCL

=0.00292 mol of HCL.

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Gay-Law Lussac's states that, for a gas with a fixed volume and number of moles, pressure is directly proportional to temperature.

The pressure exerted by the argon gas  at 60c is 0.46atm

Define pressure?

Pressure is defined as the force applied perpendicular to an object's surface per unit area across which that force is dispersed. Gauge pressure (sometimes written gauge pressure) is the pressure in relation to the surrounding atmosphere.

p is proportional to t.

p1xt1=p2xt2

where,

p1 = 0.85 atm

p2 = ?

t1 = 20c

t2 = 60c

t1=20c= (200 +275=475k)

t2=60c =(600+275=875k)

p2 =0.85x473/873

=0.4605atm

Therefore, the pressure of the gas at 60c will be 0.46atm.

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Answer: molecular equation

Explanation:

The  correct equilibrium expressions that match the given reactions are;

We know that in a chemical reaction, there is a number that shows us the extent to which the reactants have been converted into products. We know that the more the reactants are converted into products, the more this value is large and positive. On the other hand, if a lot of the reactants are not converted into products then the value would be negative and small.

This value that I have talking about is called the equilibrium constant. We can be able to obtain the equilibrium constant from the law of mass action. It is the ratio between the concentrations of the products and the reactants all raised to the power of the stoichiometric coefficients.

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By activating M₂ muscarinic receptor, acetylcholine released at the heart will slow the rate of action potential generation.

Acetylcholine is the neurotransmitter used at the neuromuscular junction—in other words, it is the chemical that motor neurons of the nervous system release in order to activate muscles. This property means that drugs that affect cholinergic systems can have very dangerous effects ranging from paralysis to convulsions.

An action potential occurs when a neuron sends information down an axon, away from the cell body. Neuroscientists use other words, such as a "spike" or an "impulse" for the action potential. The action potential is an explosion of electrical activity that is created by a depolarizing current.

Acetylcholine slows the heart rate by activating the M2 muscarinic receptor (M2R) that, in turn, opens the acetylcholine-activated potassium channel (IK, ACh) to slow the firing of the sinus node.

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The hydronium-ion concentration of the solution containing 0.0085 M Ba(OH)₂ is 0.017M and the hydroxide-ion concentration of the solution containing 0.0085 M Ba(OH)₂ is 5.88 × 10⁻¹³ M.

Given,

Ba(OH)₂ = 5.88 × 10⁻¹³ M

Dissociation of Ba(OH)₂ is given as :

Ba(OH)₂ → Ba²⁺ + OH⁻

Balanced equation:

Ba(OH)₂ → Ba²⁺ + 2OH⁻

As 1 mole of barium hydroxide produces 2 moles of hydroxide ions, so the concentration of hydroxide ion is,

[OH⁻] = 2 × [Ba(OH)₂]

[OH⁻] = 2 × 0.0085 M

[OH⁻] = 0.017 M

The concentration of hydronium ion is as follows:

[H₃O⁺] = Kw / [OH⁻]

∴ Kw = 1.0 × 10⁻¹⁴

[H₃O⁺] = 1.0 × 10⁻¹⁴ / 0.017

⇒ [H₃O⁺] = 5.88 × 10 ⁻¹³

Hence, the hydronium-ion and the hydroxide-ion concentration of the solution containing 0.0085 M Ba(OH)₂ at 25°C are 5.88 × 10 ⁻¹³ and 0.017 M respectively.

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Answer:

a thing that does things

Explanation:

A quantity that can be determined by using logarithms is a substance's pH. The base-10 logarithm can be used to calculate this. We multiply the logarithm of the hydrogen ion concentration by a negative number.

The hydrogen ion concentration of his saliva is 0.0000013 gram moles per liter.

Marco's saliva is said to have a pH of 5.9. We can solve for this number since we have an equation that determines the pH from the hydrogen ion concentration.

We must use the inverse of this logarithm to obtain the value of this variable because the pH is calculated using the logarithm with a base of 10. To do this, raise both sides by a factor of 10.

The pH of saliva typically ranges from 6.2 to 7.6, with 6.7 being the average. The mouth's pH is not below 6.3 while it is at rest. Saliva helps to keep the pH of the mouth cavity close to neutral (6.7–7.3). There are two ways that saliva helps to keep the pH balanced.

Potential Hydrogen, or pH, is an acronym that indicates how much hydrogen is present in liquids and how active the hydrogen ion is.

An acidic pH is A pH of less than 7 results in acidic saliva, which can seriously harm your smile. Your smile will likely experience acid erosion when you have acidic saliva, which can eventually cause enamel loss, tooth decay, and cavities.

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Before the reaction of hydrogen peroxide, if a reagent had changed color immediately after being added to the sample, this would indicate the presence of a contaminant and the test would be invalid.

The above experiment is also knowns as a presumptive blood testing experiment in which if a contaminant is present, the test should be deemed invalid if a vivid blue or purple color appears after adding the TMB reagent but before adding the hydrogen peroxide; this is also frequently referred to as a "false positive" result.

Presumptive tests catalyze the oxidation of a chromogenic substance, which results in a color change, by reacting with the hemoglobin of all blood in humans and animals. If the test is positive, the material will be identified as potentially blood, though not necessarily human blood.

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Silicon dioxide is found in sand, quartz glass, and silica gel packets found in packaging of shoes and electronics. the formula for silicon dioxide is SiO₂.

The formula for silicon dioxide is SiO₂.  silicon dioxide is called as silica. silicon dioxide is commonly found in quartz. it is transparent solid. it is used in adhesives, ceramics. it is also used in agriculture chemicals. white and whitish yellow as sand or powder. it is the main part in optical fiber.

Thus, Silicon dioxide is found in sand, quartz glass, and silica gel packets found in packaging of shoes and electronics. the formula for silicon dioxide is SiO₂.

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a)100464 J is the heat necessary to raise the temperature of 0.800kg of water from 0°C to 30°C .

b)125847.2x10³ J is the heat required to first melt and then raise the temperature of ice from 0°C to 30°C .

a)The concept of heat transfer which is a part of thermal properties of fluids that deals with melting, evaporation and sublimation is a prominent topic of discussion for physicists and chemists across the world. This particular concept is thus applied to solve the given question by the formula

Q=ms(ΔT)

m=mass of water

s=specific heat of water=4186 J/kg or 4.186 cal/g

Specific heat of water is the heat required to raise the temperature of 1kg water by 1°C.

T=change in temperature

=0.800 x 4186 J/kg x (30°C-0°C)

=100464 J

b) For melting and raising temperature;

Q=mLf + ms(Δ)T

Here,Lf is latent heat of fusion which is the heat required to convert solid into liquid i.e. 334 kJ/kg .

=0.800kg x 334 kJ/kg x (30°C-0°C)

=125847.2 x 10³ J

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Maximum amount of ethane formed is 9.47660606 grams .

What is Chemical reaction ?

There are chemical reactions taking place all around us. Nothing could be further from the reality, despite the fact that we occasionally link chemical processes with the sterile surroundings of the test tube and the lab. A staggering, nearly incomprehensible variety of new substances and energy changes actually result from the enormous number of alterations that occur in our environment every second of every day.

Chemical reactions happen whether or not you want them to in nature, where they can be considerably less controlled than in a lab. They can also be more messier. Whether it be a forest fire that is blazing.

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The molecular mass of ethyl acetate is 88.1 amu.

The structure formula of butanoic acid is CH3CH2CH2COOH, while structural formula of ethyl acetate is CH3COOCH2CH3. Thus, both butanoic acid and ethyl acetate have same number of constituent atoms. Therefore they have same atomic mass. Thus, molecular mass of ethyl acetate is 88.1 amu.

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Stoichiometry is the representation of balanced chemical equation, that involves the amount or quantities of reactants and products involved in the reaction.

In a chemical equation, stoichiometry is the relation between two or more substances. Stoichiometry can be the definite proportions in which elements or compounds react with one another. The rules followed in calculating the stoichiometric relationships are based on the laws of conservation of mass and energy and the law of combining weights or volumes.

For example:

N2 (g) + 3H2(g) → 2NH3(g)

In the above reaction, Nitrogen (N2) and Hydrogen (H2) react to form Ammonia (NH3).

1 molecule + 3 molecules = 2 molecules

       There is another concept known as the stoichiometric coefficient, this gives us the number of molecules that are participating in the reaction.

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Enantioselective are reactions that generate products that are enriched in one enantiomer.

Enantiomers are pairs of molecules that exist in two forms that are mirror images of each other but are not superimposable. The enantiomers are chemically identical in all other respects. Enantiomeric pairs are distinguished by the direction in which they rotate polarized light when dissolved in solution, either dextrorotatory (d or +) or levorotatory (l or -). Hence the term optical isomer.

The enantioselectivity of a chromatography system is defined as the preferential interaction of one enantiomer with a chiral selector. It is usually determined as the ratio of the retention factors of the two enantiomers in a chiral chromatography or electrophoresis system.

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If you compare 1 M Nacl solution, 1M glucose solution and 1M sucrose solution,1M Nacl solution is more hypertonic due to the dissociation of ions in the solution.

Of the two solutions the solution with higher osmotic pressure is called hypertonic. The extra pressure developed on the solution side due to the diffusion of solvent from its high amount through a semipermeable membrane is called the osmotic pressure.

    Osmotic pressure= icRT

     Where, i= van't hoff factor

                  c= concentration

                   R=Gas constant

                   t= temperature

The extent of dissociation or association of a solute in solution is expressed in terms of the van't hoff factor.

In 1M Nacl, as Nacl dissociates into ions in the solution its van't hoff factor becomes 2. In  1 M glucose it doesn't dissociate into ions. So van't hoff factor remains 1. And In 1 M sucrose,as  it does not dissociate into ions van't hoff factor  remains 1. So here Nacl has more osmotic pressure.

So, Nacl is hypertonic because of its dissociation into ions in the solution.

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If the hydrogen atom emits red, blue-green, blue, and violet light, 4 energy levels do it have in the visible region of the spectrum. Therefore, option B is correct.

The term electromagnetic spectrum can be defined as the range of frequencies of electromagnetic radiation and their respective photon energies and wavelengths.

Therefore, the four colors red, blue-green, blue, and violet light emitted by a hydrogen atom, are of four energy levels in the visible region of the electromagnetic spectrum.

Thus, option B is correct.

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Your question is incomplete, most probably your question was

If the hydrogen atom emits red, blue-green, blue, and violet light, how many energy levels does it have in the visible region of the spectrum?

A. 3

B. 4

C. 5

D. 6

Answer:

About 94

Explanation:

(1× 5.67×10²⁵)/6.03×10²³

94.03 moles of Carbon

About 94

In the reaction catalyzed by aconitase, the conversion of citrate to isocitrate is inhibited by fluoroacetate fluoroacetate is used as a pesticide this an effective pesticide because it inhibit the Krebs cycle

Aconitase catalyzes the reversible isomerization of citrate and isocitrate and it is the second enzyme in the citric acid cycle and which is a series of enzymes catalyst chemical reaction that is crucial to aerobic cellular respiration and the production of ATP and citrate is isomerized by dehydration and rehydration to isocitrate and fluoroacetate is the highly toxic towards the human and other mammals through inhibition of the enzyme aconitase in the tricarboxylic acid cycle and that's why fluoroacetate effective pesticide because it inhibit the Krebs cycle

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