You believe that the cytosolic protein p007 is involved in mediating growth hormone-stimulated proliferation in cells. You wish to establish a cell model using your cultured fibroblasts. Provide clear explanations to the following, each worth 2.5 pts each. a) Describe how you would transfect the growth hormone receptor DNA into your cells to create stable cell lines, and include a description of the benefits of using stable cell lines versus transiently transfected cell line. b) Describe how you would measure specific receptor binding of GH to your stable cell line. c) Describe how you would measure growth hormone-induced proliferation in your stable cell line. Make sure to include controls. d) Describe how you would use RNAi to demonstrate the requirement for p007 in growth-hormone stimulated proliferation. Make sure to include controls.

Answers

Answer 1

Answer:

Following are the answer to this question:

Explanation:

In option a:

There are many ways, which can be defined as follows:  

It keeping cells "qualified," use heat shock and handle ions like Ca and ice incubating.It uses Micro-injection with DNA.  It uses the biolistics or even the process of gene-gun.  

The stable cell charts indicate simple and definitive outcomes when their relationship to specific results. Moreover, relative to temporary cell lines, findings are more reliable and error-free. These lines also easily break and much less.

In option b:

Radio tests including linking tests could be used  

The Ligand Binding Assays is an analysis, that is based on the binding of ligands to antibodies, or other macromolecules. Its existence and distance of formed ligand-receptor complexes are defined using a system of measurement that is usually electrical or fluorescent.

In option c:

The method for measuring the levels of cell growth is accessible and the  Another way is to measure the metabolism within the same cell. So many color pigments were also accessible that can permeate an organism as well as react with some proteins as well as other factors, and that can effectively identify a color final product.  

Throughout relation to the amount of metabolism exercise, cell proliferation could be evaluated by researching one or even more cell markings. The BrdU limited liability assessment is a very well-published illustration.  

Its specific antibody can detect BrdU and high BrdUincorporations levels are linked to high levels of cell proliferation.  Command cell without DNA receptor growth hormone.

In option d:

The cell growth will stop after each use of RNA interference ( RNAi). The p007 formation as well as the effect on growth caused by the intrusion will be a goal. If development is delayed effectively, the theory will be tested successfully to a cell without any of the required DNA. The RNAi is the free cell


Related Questions

Why are so many of the same genes found in almost all living organisms

Answers

Answer:

Molecular homology occurs when different species inherit similar molecules, such as nucleic acids or proteins, from an evolutionary common ancestor. In many cases, especially for essential housekeeping genes involved in protein synthesis or DNA replication, genes are homologous across most, if not all, living organisms. Hope this helps!

Explanation:

Which statement correctly compares the "Analysis" and "Conclusion" sections of a lab report?

Answers

Answer:

Hello. You did not enter the answer options, but I can help you by stating that the correct comparison between "analyze" and "conclusion" is one that shows that "Analysis" is able to compare specific research data, while "conclusion" is able to suggest additional research to arrive at more concrete data.

Explanation:

In a scientific research, the "Analysis" section is where the specific research data and the interactions and information that can be extracted from them are analyzed, in a succinct and explanatory way. This section is very important to present how these data were acquired and how they work within the theme developed in the research.

The section called "conclusion", in turn, presents the section where the research will conclude the established theme, showing an affirmation discovered through the research or suggesting additional research that will help to establish an efficient conclusion.

What is it called when a bacterium uses a virus to transfer its DNA to another bacterium?
O A Transduction
х
B. Conjugation
O C. Transformation
O D. Transcription
X Incorrect
It isn’t A

Answers

it is transduction.

though it happens in prokaryotes

Answer:

Transduction.

Explanation:

A P E X

Which best defines the term matter?
Anything that can be seen
Anything that takes up space and has mass
All the living things on earth
O Anything in the atmosphere

Answers

Answer:

Anything that takes up space and has mass

Explanation:

anything that takes up space and has mass

g Cell autonomous genes affect only those cells in which they are expressed. Which group of early developmental genes are not cell autonomous?

Answers

Answer:

The options are not given..

Here are the options.

A. segment polarity genes

B. gap genes

C. coordinate genes

D. homeotic genes

The correct option is A.

segment polarity genes

Explanation:

Segment polarity genes is a group of early developmental genes that are not cell autonomous because their expression helps to define anterior and posterior polarities within each embryonic parasegment. They are the genes that help to function for the polarity within each parasegment of the embryo

Which of the following is a testable scientific question

Answers

Where are the questions so i can answer them?

Answer: Just took the test: Its "How does the age of city affect the number of trees in the city? "


is the basic unit of structure and function of living things.

Answers

Answer:

cells

Explanation:

is the basic unit of life :)

Jacqueline is a 42-year-old woman who is in her third trimester of pregnancy. Jacqueline has had three other children; their birth weights were 9.0 lbs, 9.5 lbs and 9.3 lbs. Jacqueline is a smoker with high blood pressure, and has gained 75 pounds during the last 4 months. 1. What is your diagnosis? 2. What test can you use to confirm your diagnosis? 3. What is Jacqueline's prognosis? 4. What treatments are available?

Answers

 The health condition identified is diabetes in pregnancy. To confirm my diagnosis, blood glucose test is performed.Jacqueline's prognosis, that is cause of the medical conditions, is gotten from her history of over weight babies and smoking. The available treatment is insulin injections.

Diabetes mellitus is a metabolic condition that occurs when the pancreas fails to produce enough insulin or when the body fails to use the insulin produced for glucose metabolism thereby leading to hyperglycaemia (increased blood glucose levels).

In pregnancy, diabetes is suspected in women who give this type of history:

Previous Delivery of babies weighing 9.0lbs and above.Cigarette smoking. And abrupt weight gain toward term of pregnancy.

Constant monitoring of Jacqueline is required to prevent complications during and after the pregnancy. This is done by performing series of tests and giving insulin injections when the need arises.

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the absence of the sry gene will result in: a) external genitalia of a female; internal genitalia of a male b) A phenotypic female c) and intersex individual d) A phenotypic male e) external genitalia of a male; internal genitalia of a female

Answers

Answer:

The correct answer is option b.

Explanation:

Sex determining Region of the Y chromosome gene or sry gene is a important gene found on the Y chromosome which is responsible for testis development or so the male characteristic. An XX individual with the SRY gene of the Y chromosome, will develop as male.

Hence, Absence of SRY gene will not result in phenotypic male So,option d is not correct.  intersex have male characteristic so option c is not correct.

Absence of SRY gene will lead to development of phenotypic female due to form ovary and Fallopian tube in absence of this gene

Hence, option B is correct

Which of the following best explains the potential health problems associated with endocrine disruptors? A) Endocrine disruptors are the only available pesticides,hence they are used widely. B) Endocrine disruptors mimic hormones that regulate critical biological processes. C) Endocrine disruptors do not biodegrade, remaining toxic years after their release. D) There are no major health problems associated with exposure to endocrine disruptors. E) Endocrine disruptors encompass a wide array of toxic pesticides.

Answers

B) Endocrine disruptors mimic hormones that regulate critical biological processes.

Endocrine disruptors, at times moreover called as hormonally unique trained professionals, endocrine disturbing engineered substances, or endocrine upsetting blends are manufactured substances that can interfere with endocrine structures. These aggravations can cause threatening developments, birth flees, and other developmental issues.

The potential health problems associated with endocrine disruptors is that Endocrine disruptors mimic hormones that regulate critical biological processes.

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Answer:

It's A.) Endocrine disruptors mimic hormones that regulate critical biological processes.

Explanation:

Quizlet gave me the answer

Alex, his mother, his father, and his older sister have blood type B. His younger sister and his brothers have type O. Anna, her mother, her father, and her younger brother have type A. Her sister and her older brother have type O. If Alex and Anna have three children, what is the probability at least two of them will have type O

Answers

Answer:

25%

Explanation:

the probability at least two of them will have type O can be solved as follows

Let the chances be

(Chance of Alex is I^B I ) x (1-none have type O) X (Chance of Anna is I^A i)

=>>(2/3) x (1-.75 3 ) x (2/3)= 0.25

So 0.25x 100 = 25%

The blood type of the offspring is dependent on the probable blood type

their parents.

The probability that at least 2 of them will have type O is [tex]\underline{\frac{5}{144}}[/tex]

Reasons:

Alex; Both parents are [tex]I^Bi[/tex]

Anna; Both parents are [tex]I^Ai[/tex]

Alex

[tex]\left[\begin{array}{lll}&I^B&i\\I^B&I^BI^B&I^Bi\\i&I^Bi&ii\end{array}\right][/tex]

Anna

[tex]\left[\begin{array}{lll}&I^A&i\\I^A&I^AI^A&I^Ai\\i&I^Ai&ii\end{array}\right][/tex]

Probability that [tex]I^Bi[/tex] from Alex = [tex]\frac{2}{3}[/tex]

Probability that [tex]I^Ai[/tex] from Anna = [tex]\frac{2}{3}[/tex]

Probability that two have type O = [tex]\frac{2}{3}[/tex] × [tex]\frac{2}{3}[/tex] × [tex]\left(\frac{1}{4}\right)^2[/tex] = [tex]\frac{1}{36}[/tex]

Probability that three have type O =  [tex]\frac{2}{3}[/tex] × [tex]\frac{2}{3}[/tex] × [tex]\left(\frac{1}{4}\right)^3[/tex]  =  [tex]\frac{1}{144}[/tex]

Probability that at least two have type O is

[tex]P(At \ least \ 2)=\dfrac{1}{144} + \dfrac{1}{36} = \dfrac{5}{144}[/tex]

The probability that at least 2 of them will have type O is [tex]\underline{\frac{5}{144}}[/tex]

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Please help I have one more attempt. I need help labeling the upper muscles of the arm.

Answers

There are four muscles in the upper arm. These muscles are Brachialis, Biceps brachii, triceps brachii, and coracobrachialis.

What is the function of muscles in the upper arm?

The upper arm has many muscles which are arranged in different compartments. The muscles in the upper arm are the muscles that connect the scapula with the thorax and are responsible for most of the movement of the scapula. The muscles that link the humerus are responsible for the movement of the arm. The muscles in the arm or the forearm move the arm, wrist, and hand.

The humerus is where the muscles that move your arm are located. The humerus is made up of the following muscles: Tricep, Biceps, Brachial, and Bicepsioradial. The 20-plus muscles that cause the majority of wrist, hand, and finger movement are located along the arm. The labeled image of the arm is attached below.

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during what stage of swallowing is there a risk of food entering respiratory pathways

Answers

Gulping... I think this is the stage you are looking for

Hope this helped ♥︎

The CT reveals several issues with the cranial structure, one of which might be in the cribriform plate but the image isn't well defined. What non-invasive test can you do to test if the cribriform plate is severely damaged? Why?

Answers

The non-invasive test that can be used to test if the cribriform plate is severely damaged is called the pledget study.

CRIBRIFORM PLATE is the part of the ethmoid bone that forms the nasal cavity.

The olfactory nerve which is part of the cranial nerves passes through the small opening found in the cribriform plate.

A damage to the cribriform plate leads to cerebrospinal fluid (CSF) leak which usually comes out from the nose and ears.

Therefore to test if the cribriform plate is severely damaged, the pledget study is performed.

Pledget study is a non-invasive method of diagnosing a CSF leak.

To perform this test,

Cotton pads called Pledgets are placed in the nostrils.If the leaking fluid contains CSF it will be detected.

The presence of CSF shows that there is damage to the cribriform plate.

This method is non invasive because it doesn't require the breaking or opening of any part of the body.

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The shape of the path of a planet or asteroid around the sun is an

Answers

Answer: Elliptical (similar to an oval shape)

Explanation: planets and asteroids in orbit around the sun move in a path shaped like an elliptical

Please answer ASAP, the assignments in due really soon :) 30 Points!!! The image below shows the lac operon, which breaks down lactose in bacteria. Which statement correctly describes the expression of this gene?

Answers

Answer:

B. Gene expression is influenced by high lactose levels in the environment.

Explanation:

The lactose operon (lac operon) functions for the metabolism and transport of lactose in several bacteria.

Gene expression is influenced by high lactose levels in the environment as genes are expressed in the presence of high level lactose and absence of glucose. High level lactose carry an activator protein called catabolite activator protein (CAP), which let the RNA polymerase do transcription of the operon.

Hence, the correct option is "B".

Answer:

C

Explanation:

^he said it was right^

have an amazing day! :)

Questions
41 Explain why you needed to use a water plant for this experiment​

Answers

I suspect you are referring to a specific experiment, which measures the rate of photosynthesis at differing intensities of light. This experiment could certainly be done with land plants, but using a water plant makes it easier to have a simple way to measure the rate of photosynthesis--the counting of bubbles which come off a specific area of the plant. A water plant such as elodea (the type you commonly see in aquariums) does the process of photosynthesis just as plants that grow in the air do, but you can actually see the bubbles that indicate photosynthesis is occurring--they form on the leaves before they float to the surface, enabling an easy measurement of bubbles per minute.

Plants have chloroplasts, which in addition to giving plants their green color, perform photosynthesis, the process on which life on earth is dependent. They use the energy of the sun to use carbon dioxide and water to produce sugars and oxygen. When you are counting the bubbles on the underwater leaf, you are counting bubbles of oxygen formed during photosynthesis.


A scientist wants to find the percent of teens that like the taste of "brand X"lemonade over "brand Y" lemonade. She conducts a taste test of
500 teenagers. She gives each teen a bottle of lemonade with the labels showing the brand of each lemonade. She asks each teenager to taste
each brand after drinking water in between. She finds that 50% of teens like brand X and 50% like brand Y lemonade. Why are the results
of the survey most likely unreliable?

a

Adults were not interviewed.

b

Not enough teens were interviewed.

с

The investigation was based on taste,

d

The lemonade labels introduced bias.​

Answers

Answer:

c

Explanation:

beacuse the investigation was beacesd on taste  

a report conclusion on waste management​

Answers

just some up everything you wrote into one paragraph

What happens during rusting of an iron nail?

1 sulfur and oxygen combine to form a sulfur dioxide coating

2 iron and oxygen combine to form iron oxide

3 sulfur, iron, and oxygen combine to form iron sulfoxide

4 iron and sulfur combine to form iron sulfide

Answers

Answer: the correct answer is B number 2

Explanation: I just answered this question

Which statement about malaria and sickle-cell anemia is correct? (1 point)
Malaria causes a decreased incidence of sickle-cell anemia,
Sickle-cell anemia causes an increased incidence of malaria.
Sickle-cell anemia is correlated with an increased incidence of malana
Malaria is correlated with a decreased incidence of sickle cell anemia

Answers

Answer:

sickle cell anemia cause an increase risk of malaria

Explanation:

How do malignant transformed cells differ from normal cells? They are:

Answers

Answer:

In contrast to normal cells, cancer cells don't stop growing and dividing, this uncontrolled cell growth results in the formation of a tumor. Cancer cells have more genetic changes compared to normal cells, however not all changes cause cancer, they may be a result of it

If tall is dominant over short, and yellow seed is dominant over green, how would you write the genotype of a pea plant that is heterozygous for tall, and that produces yellow seeds

Answers

Answer:

The answer has been written in paper and the image of the paper has been attached. Feel free to raise any doubt.

Determine whether the statement is true or false, and why. "Learned behavior is present from birth, requires no thought, and is
essential to survival."
A. False, it should read, "Instinctive behavior is present from birth, is developed through observation and experience, and allows animals to adapt to
changes in the environment."
False, it should read, "Instinctive behavior is not inherited, requires no thought, and is developed through experience and observation."
C. True
False, it should read, "Learned behavior is not inherited. It is developed through observation and experience and allows animals to adapt to
changes in the environment."

Answers

Answer: It is, (False, it should read, “Learned behavior is not inherited. It is developed through observation and experience and allows animals to adapt to changes in the environment.”)

Explanation:

The statement is false, it should read, "Learned behavior is not inherited. It is developed through observation and experience and allows animals to adapt to changes in the environment."

What is learned behavior?

Learned behaviors are those behaviors or characteristics that we learn from observing other people, learning and reading, and our experiences. It is the best type of characteristic of humans because learning just by observation is very efficient.

This behavior can not be inherited by another person because it is not in our genes. The behavior can be acquired by nature and the learning ability of a human, not inheritance. The genetic qualities are only inherited.

Thus, the correct option is D, false.

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The following offspring were observed from many crossings of the same pea plants. What genotypes were the parents?
465 purple axial flowers
152 purple terminal flowers
140 white axial flowers
53 white terminal flowers
a) PpAa x PpAA
b) PpAa x PpAa
c) PpAa x ppAA
d) PPAA x ppaa
e) PPaa x ppAA

Answers

Answer:

a

Explanation: the genotype is the same

Cross between same pea plants for many generations having genotype PpAa x PpAa because after crossing over parents character having a large number of phenotype in offspring, hence a is the correct option.

What is a genotype?

The term "genotype" ranges to an organism's genetic makeup, in other words, it describes an organism's whole gene. The term can also be used to refer to the alleles, or variant forms of a gene, that an organism carries in a more specific meaning.

When crossing of particular traits having genotype PpAa x PpAa because after crossing over parents' character having a large number of phenotypes in offspring.

The genotype of a person is their own DNA sequence. More specifically, the two alleles a person inherited for a particular gene are referred to using this term.

Therefore genotype parents  PpAa x PpAa offspring have a large number of traits the same as parents and a low number of new traits.

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Describe at least 2 benefits and 2 drawbacks there might be for animal cells (including humans) to make their own food through photosynthesis.

Answers

Answer:

here.

Explanation:

Benefits would be that we'd be autotrophs, meaning that we'd be independent of an external source of organic compounds for energy. All we'd need is the sun and carbon dioxide. It's a simpler and less energy-consuming process than having to undergo digestion, absorption and then assimilation.

Cons would include the fact that it provides a very limited amount of energy only for the day. Which means humans will be inactive at the night time because the energy won't be able to support our activities. Since we don't have a surface area as big as leaves, our deep cells (like those of our organs and bones) will suffer most from the lack of energy since sunlight cannot penetrate our fat, muscles and bones. This can cause organ failure if our cells solely depended on diffusion.

Our high metabolic rate and our need for energy makes photosynthesis very very ineffective.

Knowledge of the driver mutations underlying cancer has led to targeted therapeutics, such as the protein kinase inhibitor imatinib (trade name Gleevec) in cases of chronic myeloid leukemia. Cancer cells often become resistant to a given drug, so researchers continue searching for new drugs that target proteins that contribute to the cancerous phenotype. One recent promising approach uses drugs that lead to ubiquitination and proteasomal degradation of the target protein. Which of the following mutated proteins are good candidates for this approach?
A) oncogenes
B) proteins with loss-of-function mutations
C) proteins with gain-of-function mutations
D) tumor suppressor genes

Answers

Answer:

C) proteins with gain-of-function mutations

Explanation:

Gain-of-function mutations: In biology, the term "gain-of-function mutation" is described as one of the different types of mutation in which the altered or changed "gene product" consists of an entirely new pattern or molecular function associated with gene expression. However, the "gene-of-function mutations" are being always considered as "Semidominant or Dominant".

In the question above, the correct answer is option C.

Sabita is a mother in Nepal. She lives in poverty in the Western part of the country with her two children. Everyone in her family has some degree of malnutrition or nutrient deficiency. Match each of the following deficiency diseases with its corresponding nutrient.

a. Sabita is blind from a diet devoid of fortified milk, animal products, or dark yellow and orange fruits and vegetables.
b. Sabita has an enlarged thyroid gland due to a dependence on unfortified salt.

Nutrient:
1. Iron
2. Vitamin A
3. Vitamin D
4. Iodine

Answers

Answer:

a. Sabita is blind from a diet devoid of fortified milk, animal products, or dark yellow and orange fruits and vegetables. - Vitamin A

Sabita is suffering from Vitamin A deficiency. A lack of Vitamin A usually shows itself with night blindness but can cause complete blindness if not acted upon quickly.

Vitamin A deficiency is quite common in developing or poorer nations such as the area Sabita is located and usually attacks children of women in their reproductive stage. Sources of vitamin A include; fortified milk, animal products and fruits and vegetables.

b.  Sabita has an enlarged thyroid gland due to a dependence on unfortified salt. - Iodine

Iodine deficiency leads to an abnormal enlargement of the thyroid gland leading to a condition known as Goiter which presents itself as a big bulge in a person's neck. Iodine is usually placed in fortified salt along with iron to reduce iodine deficiency so Sabita's dependence on unfortified salt is robbing her of a great source of Iodine.

The condition in which the body is deprived of minerals, vitamins and other macro and micronutrients are called malnutrition.

The correct matches and their explanation are as follows:

a. The correct answer is:

Option 2. Vitamin A

Sabita is blind from meals deficient in fortified milk, animal products, fruits and vegetables due to Vitamin A deficiency.

Night blindness is a common symptom of a Vitamin A deficiency.

It is most common in developing regions due to the lack of proper meals. The best source of vitamin A is milk, fruits and animal products like eggs.

b. The correct answer is:

Option 4. Iodine

Sabita has a swollen thyroid gland due to habituation on unfortified salt deficient in Iodine.

Iodine deficient diet causes thyroid gland enlargement a disease called Goiter. This type of disease also affects glucose metabolism and weight.

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When a human egg is not fertilized, extra blood and tissue from the uterus are released through the vagina in a process called

Answers

Answer:

Unless the egg is fertilized by a sperm while in the fallopian tube, the egg leaves the body about 2 weeks later through the uterus — this is menstruation. Blood and tissues from the inner lining of the uterus combine to form the menstrual flow, which in most girls lasts from 3 to 5 days.

Please i need help !!!☹️

Answers

Answer: C. 9

Explanation:

Gestation refers to the time between when a baby is conceived till the baby is delivered. This is why the period of pregnancy is known as the Gestation Period.

During that time and after the fertilized egg implants itself in the uterus, the baby develops and grows until it is ready to be delivered.

The structure this occurs in is the uterus (womb) which is labelled 9 in the structure above.

Other Questions
Which of the following is a formal definition of jolt?A A jolt is a sharp and sudden blow, like the feeling you get when you hear the words "pop quiz."B Loena's cat landed on the table with a slight bump, but that was enough of a jolt to send her phone crashing to the floor.Jolt is both a noun and a verb, so context is needed to determine whether the word is being used to describe a suddenshock or the act of causing a shock or blow.Hot coffee sloshed into the cup holder as they jolted and bumped down the potholed road. which of the following is not a component of downstream process?a) Seperationb) Purificationc) Preservationd) Expression A toroidal solenoid has 590 turns, cross-sectional area 6.20 cm^2 , and mean radius 5.00 cm .Part A. Calcualte the coil's self-inductance.Part B. If the current decreases uniformly from 5.00 A to 2.00 A in 3.00 ms, calculate the self-induced emf in the coil.Part C. The current is directed from terminal a of the coil to terminal b. Is the direction of the induced emf froma to b or from b to a? Please Help me solve it! Please help ASAP!!!! Which of the following is a TRUE statement about the studies on obedienceconducted by Stanley Milgram, in 1963?A) He found that Americans resist obeying authority figures, but Germans do not.B) The purpose of his research was to further understand why Nazi soldiers obeyedtheir orders to commit war crimes during the HolocaustC) His studies did not involve the use of deception or confederates.D) His studies were not ethically controversial.( All of them are untrue to me) Find the equation of the line: with a slope of -1/2 through (4,5) similarities between organic and inorganic fertilizer Fomites are Group of answer choices insects that transmit pathogens from an infected host to a non infected host. animal sources for human pathogens. inanimate objects involved in the indirect contact transmission of pathogens. fecal material from infected hosts. silent carriers of infectious diseases. Personal narrative writing about a sad experience you had (trouble) and how did you feel about it.mention how you tried to solve it and how your feelings changed throughout that experience. 10. For the following isotopes that have missing information, fill in the missing informaticcomplete the notation: 36P . Error Analysis Describe and correct the errora student made when ordering numbers fromleast to greatest.HELP ASAP plzzzzzzz help asap!!!!!! T.T the different between microspores and pollen grain T ____________________ de Estados Unidos. a. somos c. es b. son d. eres There were 18,652 geese on a lake. What is this number rounded to the tenthousands place? a 2-n force is applied to a spring, and there is displacement of 0.4 m. how much would the spring be displaced if a 5-n force was applied? Which transformation shows a reflection across the y-axis? A) A B) B C) C D) D 2. Name the sympathetic ganglia in which the preganglionic and postganglionic neurons synapse. how was teamwork used in sonata for harp and bicycle